Abstract
High-affinity nitrate transporters (NRT) are key components for nitrogen (N) acquisition and distribution within plants. However, insights on these transporters in wheat are scarce. This study presents a comprehensive analysis of the NRT2 and NRT3 gene families, where the aim is to shed light on their functionality and to evaluate their responses to N availability. A total of 53 NRT2s and 11 NRT3s were identified in the bread wheat genome, and these were grouped into different clades and homoeologous subgroups. The transcriptional dynamics of the identified NRT2 and NRT3 genes, in response to N starvation and nitrate resupply, were examined by RT-qPCR in the roots and shoots of hydroponically grown wheat plants through a time course experiment. Additionally, the spatial expression patterns of these genes were explored within the plant. The NRT2s of clade 1, TaNRT2.1-2.6, showed a root-specific expression and significant upregulation in response to N starvation, thus emphasizing a role in N acquisition. However, most of the clade 2 NRT2s displayed reduced expression under N-starved conditions. Nitrate resupply after N starvation revealed rapid responsiveness in TaNRT2.1-2.6, while clade 2 genes exhibited gradual induction, primarily in the roots. TaNRT2.18 was highly expressed in above-ground tissues and exhibited distinct nitrate-related response patterns for roots and shoots. The TaNRT3 gene expression closely paralleled the profiles of TaNRT2.1-2.6 in response to nitrate induction. These findings enhance the understanding of NRT2 and NRT3 involvement in nitrogen uptake and utilization, and they could have practical implications for improving nitrogen use efficiency. The study also recommends a standardized nomenclature for wheat NRT2 genes, thereby addressing prior naming inconsistencies.
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Claims 12
Claim · model-written summary
The wheat genome contained 53 NRT2 genes.
- Taxon
- bread wheat
Triticum aestivume0 - Gene
- NRT2se0
- Outcome
- NRT2se0
- Value
- 53 NRT2se0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 53 NRT2s and 11 NRT3s were identified in the bread wheat genome”
Claim · model-written summary
The wheat genome contained 11 NRT3 genes.
- Taxon
- bread wheatTriticum aestivume0
- Gene
- NRT3se0
- Outcome
- NRT3s were identifiede0
- Value
- 11 NRT3se0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 53 NRT2s and 11 NRT3s were identified in the bread wheat genome”
Claim · model-written summary
Identified NRT2 and NRT3 genes formed distinct clades and homoeologous subgroups.
- Taxon
- bread wheatTriticum aestivume2
- Gene
- NRT2 and NRT3e1
- Outcome
- grouped into different clades and homoeologous subgroupse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these were grouped into different clades and homoeologous subgroups”
e1“NRT2 and NRT3 gene families”
e2“bread wheat genome”
Claim · model-written summary
Clade 1 TaNRT2.1-2.6 showed root-specific expression.
- Taxon
- wheatTriticum aestivume1
- Gene
- TaNRT2.1-2.6e0
- Outcome
- root-specific expressione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The NRT2s of clade 1, TaNRT2.1-2.6, showed a root-specific expression and significant upregulation in response to N starvation”
e1“wheat plants”
Claim · model-written summary
Nitrogen starvation significantly upregulated TaNRT2.1-2.6.
- Taxon
- wheatTriticum aestivume1
- Treatment
- N starvatione0
- Gene
- TaNRT2.1-2.6e0
- Outcome
- expressione0
- Direction
- upregulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The NRT2s of clade 1, TaNRT2.1-2.6, showed a root-specific expression and significant upregulation in response to N starvation”
e1“wheat plants”
Claim · model-written summary
Most clade 2 NRT2 genes showed reduced expression during nitrogen starvation.
- Taxon
- wheatTriticum aestivume1
- Treatment
- N-starved conditionse0
- Gene
- clade 2 NRT2se0
- Outcome
- expressione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“most of the clade 2 NRT2s displayed reduced expression under N-starved conditions”
e1“wheat plants”
Claim · model-written summary
TaNRT2.1-2.6 responded rapidly to nitrate resupply after starvation.
- Taxon
- wheatTriticum aestivume1
- Treatment
- Nitrate resupply after N starvatione0
- Gene
- TaNRT2.1-2.6e0
- Outcome
- rapid responsivenesse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nitrate resupply after N starvation revealed rapid responsiveness in TaNRT2.1-2.6”
e1“wheat plants”
Claim · model-written summary
Clade 2 genes were gradually induced by nitrate resupply, mainly in roots.
- Taxon
- wheatTriticum aestivume2
- Organ / stage
- rootse0
- Treatment
- Nitrate resupply after N starvatione1
- Gene
- clade 2 genese0
- Outcome
- inductione0
- Direction
- gradual inductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“clade 2 genes exhibited gradual induction, primarily in the roots”
e1“Nitrate resupply after N starvation”
e2“wheat plants”
Claim · model-written summary
TaNRT2.18 was highly expressed in above-ground tissues.
- Taxon
- wheatTriticum aestivume1
- Organ / stage
- above-ground tissuese0
- Gene
- TaNRT2.18e0
- Outcome
- expressede0
- Direction
- highlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaNRT2.18 was highly expressed in above-ground tissues and exhibited distinct nitrate-related response patterns for roots and shoots”
e1“wheat plants”
Claim · model-written summary
TaNRT2.18 had distinct nitrate responses in roots and shoots.
- Taxon
- wheatTriticum aestivume1
- Gene
- TaNRT2.18e0
- Outcome
- distinct nitrate-related response patterns for roots and shootse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaNRT2.18 was highly expressed in above-ground tissues and exhibited distinct nitrate-related response patterns for roots and shoots”
e1“wheat plants”
Claim · model-written summary
TaNRT3 expression closely paralleled TaNRT2.1-2.6 profiles during nitrate induction.
- Taxon
- wheatTriticum aestivume1
- Treatment
- nitrate inductione0
- Gene
- TaNRT3e0
- Outcome
- gene expressione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The TaNRT3 gene expression closely paralleled the profiles of TaNRT2.1-2.6 in response to nitrate induction”
e1“wheat plants”
Claim · model-written summary
The authors recommend standardized nomenclature for wheat NRT2 genes.
- Taxon
- wheatTriticum aestivume0
- Gene
- NRT2e0
- Outcome
- standardized nomenclature for wheat NRT2 genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The study also recommends a standardized nomenclature for wheat NRT2 genes”
The same questions in other papers
nitrogen · molecular: expression · Triticum aestivum (wheat)Positive and negative results
10 papers in the same direction as this paper · 26 in the opposite direction
This paper is about lacking the nutrient, so it counts the other way round here.
- positive
- positive
- positive
33 papers more in this group
See all 37 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.