Abstract
The chilling stress induced by the global climate change harms rice production, especially at seedling and booting stage, which feed half the population of the world. Although there are key quantitative trait locus genes identified in the individual stage, few genes have been reported and functioned at both stages. Utilizing chromosome segment substitution lines (CSSLs) and a combination of map-based cloning and phenotypes of the mutants and overexpression lines, we identified the major gene Chilling-tolerance in Geng/japonica rice 3 (COG3) of q chilling-tolerance at the booting and seedling stage 11 (qCTBS11) conferred chilling tolerance at both seedling and booting stages. COG3 was significantly upregulated in Nipponbare under chilling treatment compared with its expression in 93-11. The loss-of-function mutants cog3 showed a reduced chilling tolerance. On the contrary, overexpression enhanced chilling tolerance. Genome evolution and genetic analysis suggested that COG3 may have undergone strong selection in temperate japonica during domestication. COG3, a putative calmodulin-binding protein, physically interacted with OsFtsH2 at chloroplast. In cog3-1, OsFtsH2-mediated D1 degradation was impaired under chilling treatment compared with wild-type. Our results suggest that COG3 is necessary for maintaining OsFtsH2 protease activity to regulate chilling tolerance at the booting and seedling stage.
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Claims 8
Claim · model-written summary
COG3 at qCTBS11 conferred chilling tolerance at seedling and booting stages.
- Taxon
- rice
Oryza sativae0 - Organ / stage
- seedling and booting stagese0
- Gene
- COG3e0
- Outcome
- chilling tolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified the major gene Chilling-tolerance in Geng/japonica rice 3 (COG3) of q chilling-tolerance at the booting and seedling stage 11 (qCTBS11) conferred chilling tolerance at both seedling and booting stages”
Claim · model-written summary
Chilling significantly upregulated COG3 in Nipponbare relative to 93-11.
- Taxon
- riceOryza sativat0
- Material
- Nipponbaree0
- Treatment
- chilling treatmente0
- Gene
- COG3e0
- Outcome
- COG3e0
- Direction
- upregulatede0
- Comparator
- 93-11e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“COG3 was significantly upregulated in Nipponbare under chilling treatment compared with its expression in 93-11”
t0“rice”
Claim · model-written summary
COG3 loss of function reduced chilling tolerance.
- Taxon
- riceOryza sativat0
- Material
- cog3e0
- Treatment
- loss-of-functione0
- Gene
- cog3e0
- Outcome
- chilling tolerancee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The loss-of-function mutants cog3 showed a reduced chilling tolerance”
t0“rice”
Claim · model-written summary
COG3 overexpression enhanced chilling tolerance.
- Taxon
- riceOryza sativat0
- Treatment
- overexpressione0
- Gene
- COG3t0
- Outcome
- chilling tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On the contrary, overexpression enhanced chilling tolerance”
t0“COG3 confers the chilling tolerance to mediate OsFtsH2‐D1 module in rice”
Claim · model-written summary
COG3 may have undergone strong selection in temperate japonica domestication.
- Taxon
- riceOryza sativat0
- Material
- temperate japonicae0
- Gene
- COG3e0
- Outcome
- strong selectione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“COG3 may have undergone strong selection in temperate japonica during domestication”
t0“rice”
Claim · model-written summary
COG3 physically interacted with OsFtsH2 at chloroplasts.
- Taxon
- riceOryza sativat0
- Organ / stage
- chloroplaste0
- Gene
- COG3e0
- Outcome
- physically interacted with OsFtsH2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“COG3, a putative calmodulin-binding protein, physically interacted with OsFtsH2 at chloroplast”
t0“rice”
Claim · model-written summary
OsFtsH2-mediated D1 degradation was impaired in cog3-1 under chilling.
- Taxon
- riceOryza sativat0
- Material
- cog3-1e0
- Treatment
- chilling treatmente0
- Gene
- OsFtsH2e0
- Outcome
- D1 degradatione0
- Direction
- impairede0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In cog3-1, OsFtsH2-mediated D1 degradation was impaired under chilling treatment compared with wild-type”
t0“rice”
Claim · model-written summary
COG3 is proposed to maintain OsFtsH2 protease activity for chilling tolerance.
- Taxon
- riceOryza sativat0
- Organ / stage
- booting and seedling stagee0
- Gene
- COG3e0
- Outcome
- chilling tolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“COG3 is necessary for maintaining OsFtsH2 protease activity to regulate chilling tolerance at the booting and seedling stage”
t0“rice”
The same questions in other papers
loss of function · tolerance: abiotic · Oryza sativa (rice)Positive and negative results
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- positive
- positive
- positive
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See all 31 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.