Abstract
Iron deficiency is a major nutritional problem causing iron deficiency chlorosis (IDC) and yield reduction in soybean, one of the most important crops. The ATP-binding cassette G subfamily plays a crucial role in substance transportation in plants. In this study, we cloned the GmABCG5 gene from soybean and verified its role in Fe homeostasis. Analysis showed that GmABCG5 belongs to the ABCG subfamily and is subcellularly localized at the cell membrane. From high to low, GmABCG5 expression was found in the stem, root, and leaf of young soybean seedlings, and the order of expression was flower, pod, seed stem, root, and leaf in mature soybean plants. The GUS assay and qRT-PCR results showed that the GmABCG5 expression was significantly induced by iron deficiency in the leaf. We obtained the GmABCG5 overexpressed and inhibitory expressed soybean hairy root complexes. Overexpression of GmABCG5 promoted, and inhibition of GmABCG5 retarded the growth of soybean hairy roots, independent of nutrient iron conditions, confirming the growth-promotion function of GmABCG5 . Iron deficiency has a negative effect on the growth of soybean complexes, which was more obvious in the GmABCG5 inhibition complexes. The chlorophyll content was increased in the GmABCG5 overexpression complexes and decreased in the GmABCG5 inhibition complexes. Iron deficiency treatment widened the gap in the chlorophyll contents. FCR activity was induced by iron deficiency and showed an extraordinary increase in the GmABCG5 overexpression complexes, accompanied by the greatest Fe accumulation. Antioxidant capacity was enhanced when GmABCG5 was overexpressed and reduced when GmABCG5 was inhibited under iron deficiency. These results showed that the response mechanism to iron deficiency is more actively mobilized in GmABCG5 overexpression seedlings. Our results indicated that GmABCG5 could improve the plant’s tolerance to iron deficiency, suggesting that GmABCG5 might have the function of Fe mobilization, redistribution, and/or secretion of Fe substances in plants. The findings provide new insights into the ABCG subfamily genes in the regulation of iron homeostasis in plants.
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Claims 16
Claim · model-written summary
GmABCG5 belongs to the ABCG subfamily and localizes at the cell membrane.
- Taxon
- soybean
Glycine maxt0 - Gene
- GmABCG5e0
- Outcome
- ABCG subfamily and is subcellularly localized at the cell membranee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmABCG5 belongs to the ABCG subfamily and is subcellularly localized at the cell membrane”
t0“soybean”
Claim · model-written summary
Young soybean seedling GmABCG5 expression ranked stem, root, leaf.
- Taxon
- soybeanGlycine maxe0
- Organ / stage
- stem, root, and leafe0
- Gene
- GmABCG5e0
- Outcome
- GmABCG5 expressione0
- Direction
- high to lowe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“From high to low, GmABCG5 expression was found in the stem, root, and leaf of young soybean seedlings”
Claim · model-written summary
Mature soybean GmABCG5 expression ranked flower, pod, seed stem, root, leaf.
- Taxon
- soybeanGlycine maxe0
- Organ / stage
- flower, pod, seed stem, root, and leafe0
- Gene
- GmABCG5t0
- Outcome
- order of expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the order of expression was flower, pod, seed stem, root, and leaf in mature soybean plants”
t0“GmABCG5, an ATP-binding cassette G transporter gene, is involved in the iron deficiency response in soybean”
Claim · model-written summary
Iron deficiency significantly induced GmABCG5 expression in leaves.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- leafe0
- Treatment
- iron deficiencye0
- Gene
- GmABCG5e0
- Outcome
- GmABCG5 expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmABCG5 expression was significantly induced by iron deficiency in the leaf”
t0“soybean”
Claim · model-written summary
GmABCG5 overexpression promoted hairy root growth.
- Taxon
- soybeanGlycine maxe0
- Treatment
- Overexpression of GmABCG5e0
- Gene
- GmABCG5e0
- Outcome
- growth of soybean hairy rootse0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of GmABCG5 promoted, and inhibition of GmABCG5 retarded the growth of soybean hairy roots, independent of nutrient iron conditions”
Claim · model-written summary
GmABCG5 inhibition retarded hairy root growth.
- Taxon
- soybeanGlycine maxe0
- Treatment
- inhibition of GmABCG5e0
- Gene
- GmABCG5e0
- Outcome
- growth of soybean hairy rootse0
- Direction
- retardede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inhibition of GmABCG5 retarded the growth of soybean hairy roots, independent of nutrient iron conditions”
Claim · model-written summary
Iron deficiency reduced soybean complex growth, especially with GmABCG5 inhibition.
- Taxon
- soybeanGlycine maxe0
- Treatment
- Iron deficiencye0
- Outcome
- growth of soybean complexese0
- Direction
- negativee0
- Qualifier
- GmABCG5 inhibition complexese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Iron deficiency has a negative effect on the growth of soybean complexes, which was more obvious in the GmABCG5 inhibition complexes”
Claim · model-written summary
GmABCG5 overexpression increased chlorophyll content.
- Taxon
- soybeanGlycine maxt0
- Treatment
- GmABCG5 overexpression complexese0
- Gene
- GmABCG5e0
- Outcome
- chlorophyll contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The chlorophyll content was increased in the GmABCG5 overexpression complexes and decreased in the GmABCG5 inhibition complexes”
t0“soybean”
Claim · model-written summary
GmABCG5 inhibition decreased chlorophyll content.
- Taxon
- soybeanGlycine maxt0
- Treatment
- GmABCG5 inhibition complexese0
- Gene
- GmABCG5e0
- Outcome
- chlorophyll contente0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The chlorophyll content was increased in the GmABCG5 overexpression complexes and decreased in the GmABCG5 inhibition complexes”
t0“soybean”
Claim · model-written summary
Iron deficiency widened the chlorophyll content gap.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Iron deficiency treatmente0
- Outcome
- gap in the chlorophyll contentse0
- Direction
- widenede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Iron deficiency treatment widened the gap in the chlorophyll contents”
t0“soybean”
Claim · model-written summary
Iron deficiency induced FCR activity.
- Taxon
- soybeanGlycine maxt0
- Treatment
- iron deficiencye0
- Outcome
- FCR activitye0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FCR activity was induced by iron deficiency and showed an extraordinary increase in the GmABCG5 overexpression complexes, accompanied by the greatest Fe accumulation”
t0“soybean”
Claim · model-written summary
GmABCG5 overexpression strongly increased FCR activity.
- Taxon
- soybeanGlycine maxt0
- Treatment
- GmABCG5 overexpression complexese0
- Outcome
- FCR activitye0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FCR activity was induced by iron deficiency and showed an extraordinary increase in the GmABCG5 overexpression complexes, accompanied by the greatest Fe accumulation”
t0“soybean”
Claim · model-written summary
GmABCG5 overexpression complexes accumulated the most Fe.
- Taxon
- soybeanGlycine maxt0
- Material
- GmABCG5 overexpression complexese0
- Outcome
- Fe accumulatione0
- Direction
- greateste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmABCG5 overexpression complexes, accompanied by the greatest Fe accumulation”
t0“soybean”
Claim · model-written summary
GmABCG5 overexpression enhanced antioxidant capacity under iron deficiency.
- Taxon
- soybeanGlycine maxt0
- Treatment
- GmABCG5 was overexpressede0
- Outcome
- Antioxidant capacitye0
- Direction
- enhancede0
- Qualifier
- under iron deficiencye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antioxidant capacity was enhanced when GmABCG5 was overexpressed and reduced when GmABCG5 was inhibited under iron deficiency”
t0“soybean”
Claim · model-written summary
GmABCG5 inhibition reduced antioxidant capacity under iron deficiency.
- Taxon
- soybeanGlycine maxt0
- Treatment
- GmABCG5 was inhibitede0
- Outcome
- Antioxidant capacitye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antioxidant capacity was enhanced when GmABCG5 was overexpressed and reduced when GmABCG5 was inhibited under iron deficiency”
t0“soybean”
Claim · model-written summary
Authors infer that GmABCG5 improves tolerance to iron deficiency.
- Taxon
- soybeanGlycine maxt0
- Gene
- GmABCG5e0
- Outcome
- tolerance to iron deficiencye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmABCG5 could improve the plant’s tolerance to iron deficiency”
t0“soybean”
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