Abstract
Plant Myeloblastosis (MYB) proteins function crucially roles upon variegated abiotic stresses. Nonetheless, their effects and mechanisms in rose (Rosa chinensis) are not fully clarified. In this study, we characterized the effects of rose RcMYB8 under salt and drought tolerances. For induction of the RcMYB8 expression, NaCl and drought stress treatment were adopted. Rose plants overexpressing RcMYB8 displayed enhanced tolerance to salinity and drought stress, while silencing RcMYB8 resulted in decreased tolerance, as evidenced by lowered intra-leaf electrolyte leakage and callose deposition, as well as photosynthetic sustainment under stressed conditions. Here, we further show that RcMYB8 binds similarly to the promoters of RcPR5/1 and RcP5C51 in vivo and in vitro. Inhibiting RcP5CS1 by virus-induced gene silencing led to decreased drought tolerance through the reactive oxygen species (ROS) homeostatic regulation. RcP5CS1-silenced plants showed an increase in ion leakage and reduce of proline content, together with the content of malondialdehyde (MDA) increased, lowered activities of Catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD). Our study highlights the transcriptional modulator role of RcMYB8 in drought and salinity tolerances, which bridges RcPR5/1 and RcP5CS1 by promoting ROS scavenging. Besides, it is probably applicable to the rose plant engineering for enhancing their abiotic stress tolerances.
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Claims 5
Claim · model-written summary
RcMYB8 overexpression enhanced rose tolerance to salinity and drought stress.
- Taxon
- Rosee0
- Treatment
- overexpressing RcMYB8e0
- Gene
- RcMYB8e0
- Outcome
- tolerance to salinity and drought stresse0
- Direction
- enhancede0
Not reportedComparator
Verbatim
e0“Rose plants overexpressing RcMYB8 displayed enhanced tolerance to salinity and drought stress, while silencing RcMYB8 resulted in decreased tolerance”
Claim · model-written summary
RcMYB8 silencing decreased rose tolerance to salinity and drought stress.
Not reportedComparator
Verbatim
e0“Rose plants overexpressing RcMYB8 displayed enhanced tolerance to salinity and drought stress, while silencing RcMYB8 resulted in decreased tolerance”
Claim · model-written summary
RcMYB8 bound the promoters of RcPR5/1 and RcP5C51 in vivo and in vitro.
Not reportedTaxon
Verbatim
e0“RcMYB8 binds similarly to the promoters of RcPR5/1 and RcP5C51 in vivo and in vitro”
Claim · model-written summary
Silencing RcP5CS1 decreased rose drought tolerance through reactive oxygen species homeostasis.
- Treatment
- virus-induced gene silencinge0
- Gene
- RcP5CS1e0
- Outcome
- drought tolerancee0
- Direction
- decreasede0
- Qualifier
- reactive oxygen species (ROS) homeostatic regulatione0
Not reportedTaxonComparator
Verbatim
e0“Inhibiting RcP5CS1 by virus-induced gene silencing led to decreased drought tolerance through the reactive oxygen species (ROS) homeostatic regulation.”
Claim · model-written summary
RcP5CS1 silencing increased ion leakage and malondialdehyde and reduced proline content and antioxidant enzyme activities.
Not reportedTaxonComparator
Verbatim
e0“RcP5CS1-silenced plants showed an increase in ion leakage and reduce of proline content, together with the content of malondialdehyde (MDA) increased, lowered activities of Catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD).”
The same questions in other papers
loss of function · disease · RosaPositive and negative results
5 papers in the same direction as this paper · 2 in the opposite direction
- negative
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.