Abstract
Bud endodormancy represents a pivotal and intricate biological process influenced by both genetic and epigenetic factors, the exact mechanism of which remains elusive. Hydrogen peroxide (H2O2) functions as a signalling molecule in the regulation of dormancy, with peroxidase (POD) playing a crucial role in governing H2O2 levels. Our prior transcriptomic and metabolomic investigations into diverse pear dormancy phases posited that POD predominantly oversees pear bud dormancy. In this study, we utilised qRT-PCR to screen the most significantly expressed gene, Pyrus pyrifolia POD4-like (PpPOD4-like), from seven POD genes. Subsequently, H2O2 test kits, overexpression methods, and subcellular localisation techniques were employed to assess changes in H2O2 content, POD activity, PpPOD4-like expression, and its cellular positioning during pear bud dormancy. Subcellular localisation experiments revealed that PpPOD4-like is situated on the cell membranes. Notably, H2O2 content exhibited a rapid increase during endodormancy and decreased swiftly after ecodormancy. The fluctuation pattern of POD activity aligned with that of H2O2 content. Additionally, PpPOD4-like expression was markedly upregulated, displaying an overall upward trajectory. Our findings indicate that PpPOD4-like modulates H2O2 levels by regulating POD activity, thereby actively participating in the intricate regulation of pear dormancy processes.
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Claims 7
Claim · model-written summary
PpPOD4-like was the most significantly expressed of seven POD genes.
- Taxon
- Pyrus pyrifolia
_Pyrus_pyrifolia_flowers.jpg?width=60)
Pyrus pyrifoliae0 - Gene
- Pyrus pyrifolia POD4-likee0
- Outcome
- most significantly expressed genee0
- Value
- seven POD genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Pyrus_pyrifolia_flowers.jpg?width=500)
Verbatim
e0“screen the most significantly expressed gene, Pyrus pyrifolia POD4-like (PpPOD4-like), from seven POD genes”
Claim · model-written summary
PpPOD4-like localized to cell membranes.
- Taxon
- Pyrus pyrifoliaPyrus pyrifoliat0
- Gene
- PpPOD4-likee0
- Outcome
- situated on the cell membranese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PpPOD4-like is situated on the cell membranes”
t0“Pear (Pyrus pyrifolia)”
Claim · model-written summary
Hydrogen peroxide content increased rapidly during pear bud endodormancy.
- Taxon
- Pyrus pyrifoliaPyrus pyrifoliat0
- Organ / stage
- endodormancye0
- Outcome
- H2O2 contente0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H2O2 content exhibited a rapid increase during endodormancy”
t0“Pear (Pyrus pyrifolia)”
Claim · model-written summary
Hydrogen peroxide content decreased swiftly after pear bud ecodormancy.
- Taxon
- Pyrus pyrifoliaPyrus pyrifoliat0
- Organ / stage
- ecodormancye0
- Outcome
- H2O2 contente0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H2O2 content exhibited a rapid increase during endodormancy and decreased swiftly after ecodormancy”
t0“Pear (Pyrus pyrifolia)”
Claim · model-written summary
POD activity followed the fluctuation pattern of hydrogen peroxide content.
- Taxon
- Pyrus pyrifoliaPyrus pyrifoliat0
- Gene
- PODe0
- Outcome
- POD activitye0
- Comparator
- H2O2 contente0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The fluctuation pattern of POD activity aligned with that of H2O2 content”
t0“Pear (Pyrus pyrifolia)”
Claim · model-written summary
PpPOD4-like expression was markedly upregulated overall.
- Taxon
- Pyrus pyrifoliaPyrus pyrifoliat0
- Gene
- PpPOD4-likee0
- Outcome
- PpPOD4-like expressione0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PpPOD4-like expression was markedly upregulated, displaying an overall upward trajectory”
t0“Pear (Pyrus pyrifolia)”
Claim · model-written summary
The authors infer that PpPOD4-like modulates hydrogen peroxide through POD activity in pear dormancy.
- Taxon
- Pyrus pyrifoliaPyrus pyrifoliat0
- Gene
- PpPOD4-likee0
- Outcome
- H2O2 levelse0
- Qualifier
- by regulating POD activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PpPOD4-like modulates H2O2 levels by regulating POD activity, thereby actively participating in the intricate regulation of pear dormancy processes”
t0“Pear (Pyrus pyrifolia)”