Abstract
The recretohalophyte Limonium bicolor thrives in high-salinity environments because salt glands on the above-ground parts of the plant help to expel excess salt. Here, we characterize a nucleus-localized C3HC4 (RING-HC)-type zinc finger protein of L. bicolor named RING ZINC FINGER PROTEIN 1 (LbRZF1). LbRZF1 was expressed in salt glands and in response to NaCl treatment. LbRZF1 showed no E3 ubiquitin ligase activity. The phenotypes of overexpression and knockout lines for LbRZF1 indicated that LbRZF1 positively regulated salt gland development and salt tolerance in L. bicolor. lbrzf1 mutants had fewer salt glands and secreted less salt than did the wild-type, whereas LbRZF1-overexpressing lines had opposite phenotypes, in keeping with the overall salt tolerance of these plants. A yeast two-hybrid screen revealed that LbRZF1 interacted with LbCATALASE2 (LbCAT2) and the transcription factor LbMYB113, leading to their stabilization. Silencing of LbCAT2 or LbMYB113 decreased salt gland density and salt tolerance. The heterologous expression of LbRZF1 in Arabidopsis thaliana conferred salt tolerance to this non-halophyte. We also identified the transcription factor LbMYB48 as an upstream regulator of LbRZF1 transcription. The study of LbRZF1 in the regulation network of salt gland development also provides a good foundation for transforming crops and improving their salt resistance.
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Claims 13
Claim · model-written summary
LbRZF1 is expressed in salt glands of Limonium bicolor.
- Taxon
- Limonium bicolorLimonium bicolore0
- Organ / stage
- salt glandse1
- Gene
- LbRZF1e1
- Outcome
- expressed in salt glandse1
Not reportedDirection
Verbatim
e0“The recretohalophyte Limonium bicolor”
e1“LbRZF1 was expressed in salt glands and in response to NaCl treatment”
Claim · model-written summary
LbRZF1 expression responds to NaCl treatment.
- Taxon
- Limonium bicolorLimonium bicolore0
- Treatment
- NaCl treatmente1
- Gene
- LbRZF1e1
- Outcome
- expressede1
Not reportedComparatorDirection
Verbatim
e0“The recretohalophyte Limonium bicolor”
e1“LbRZF1 was expressed in salt glands and in response to NaCl treatment”
Claim · model-written summary
LbRZF1 shows no E3 ubiquitin ligase activity.
- Taxon
- Limonium bicolorLimonium bicolore0
- Gene
- LbRZF1e2
- Outcome
- E3 ubiquitin ligase activitye2
Not reportedDirection
Verbatim
e0“The recretohalophyte Limonium bicolor”
e2“LbRZF1 showed no E3 ubiquitin ligase activity”
Claim · model-written summary
Overexpression and knockout lines indicate that LbRZF1 positively regulates salt gland development.
- Taxon
- Limonium bicolorLimonium bicolore0
- Treatment
- overexpression and knockout lines for LbRZF1e3
- Gene
- LbRZF1e3
- Outcome
- salt gland developmente3
- Direction
- positively regulatede3
Not reportedComparator
Verbatim
e0“The recretohalophyte Limonium bicolor”
e3“The phenotypes of overexpression and knockout lines for LbRZF1 indicated that LbRZF1 positively regulated salt gland development and salt tolerance in L. bicolor. lbrzf1 mutants had fewer salt glands and secreted less salt than did the wild-type, whereas LbRZF1-overexpressing lines had opposite phenotypes, in keeping with the overall salt tolerance of these plants”
Claim · model-written summary
Overexpression and knockout lines indicate that LbRZF1 positively regulates salt tolerance.
- Taxon
- Limonium bicolorLimonium bicolore0
- Treatment
- overexpression and knockout lines for LbRZF1e3
- Gene
- LbRZF1e3
- Outcome
- salt tolerancee3
- Direction
- positively regulatede3
Not reportedComparator
Verbatim
e0“The recretohalophyte Limonium bicolor”
e3“The phenotypes of overexpression and knockout lines for LbRZF1 indicated that LbRZF1 positively regulated salt gland development and salt tolerance in L. bicolor. lbrzf1 mutants had fewer salt glands and secreted less salt than did the wild-type, whereas LbRZF1-overexpressing lines had opposite phenotypes, in keeping with the overall salt tolerance of these plants”
Claim · model-written summary
lbrzf1 mutants have fewer salt glands than the wild-type.
Verbatim
e0“The recretohalophyte Limonium bicolor”
e3“The phenotypes of overexpression and knockout lines for LbRZF1 indicated that LbRZF1 positively regulated salt gland development and salt tolerance in L. bicolor. lbrzf1 mutants had fewer salt glands and secreted less salt than did the wild-type, whereas LbRZF1-overexpressing lines had opposite phenotypes, in keeping with the overall salt tolerance of these plants”
Claim · model-written summary
lbrzf1 mutants secrete less salt than the wild-type.
Verbatim
e0“The recretohalophyte Limonium bicolor”
e3“The phenotypes of overexpression and knockout lines for LbRZF1 indicated that LbRZF1 positively regulated salt gland development and salt tolerance in L. bicolor. lbrzf1 mutants had fewer salt glands and secreted less salt than did the wild-type, whereas LbRZF1-overexpressing lines had opposite phenotypes, in keeping with the overall salt tolerance of these plants”
Claim · model-written summary
LbRZF1-overexpressing lines show opposite phenotypes, in keeping with their overall salt tolerance.
- Taxon
- Limonium bicolorLimonium bicolore0
- Treatment
- LbRZF1-overexpressing linese3
- Gene
- LbRZF1e3
- Outcome
- phenotypese3
- Direction
- oppositee3
- Qualifier
- in keeping with the overall salt tolerance of these plantse3
Not reportedComparator
Verbatim
e0“The recretohalophyte Limonium bicolor”
e3“The phenotypes of overexpression and knockout lines for LbRZF1 indicated that LbRZF1 positively regulated salt gland development and salt tolerance in L. bicolor. lbrzf1 mutants had fewer salt glands and secreted less salt than did the wild-type, whereas LbRZF1-overexpressing lines had opposite phenotypes, in keeping with the overall salt tolerance of these plants”
Claim · model-written summary
Silencing of LbCAT2 decreased salt gland density and salt tolerance.
- Taxon
- Limonium bicolorLimonium bicolore0
- Treatment
- Silencing of LbCAT2 or LbMYB113e4
- Gene
- LbCAT2e4
- Outcome
- salt gland density and salt tolerancee4
- Direction
- decreasede4
Not reportedComparator
Verbatim
e0“The recretohalophyte Limonium bicolor”
e4“Silencing of LbCAT2 or LbMYB113 decreased salt gland density and salt tolerance”
Claim · model-written summary
Silencing of LbMYB113 decreased salt gland density and salt tolerance.
- Taxon
- Limonium bicolorLimonium bicolore0
- Treatment
- Silencing of LbCAT2 or LbMYB113e4
- Gene
- LbMYB113e4
- Outcome
- salt gland density and salt tolerancee4
- Direction
- decreasede4
Not reportedComparator
Verbatim
e0“The recretohalophyte Limonium bicolor”
e4“Silencing of LbCAT2 or LbMYB113 decreased salt gland density and salt tolerance”
Claim · model-written summary
LbRZF1 interacts with LbCAT2 and LbMYB113, leading to their stabilization.
- Taxon
- Limonium bicolorLimonium bicolore0
- Gene
- LbRZF1e5
- Outcome
- their stabilizatione5
- Qualifier
- interacted with LbCATALASE2 (LbCAT2)e5
Verbatim
e0“The recretohalophyte Limonium bicolor”
e5“A yeast two-hybrid screen revealed that LbRZF1 interacted with LbCATALASE2 (LbCAT2) and the transcription factor LbMYB113, leading to their stabilization”
Claim · model-written summary
LbMYB48 is an upstream regulator of LbRZF1 transcription.
- Taxon
- Limonium bicolorLimonium bicolore0
- Gene
- LbMYB48e6
- Outcome
- LbRZF1 transcriptione6
- Qualifier
- upstream regulator of LbRZF1 transcriptione6
Verbatim
e0“The recretohalophyte Limonium bicolor”
e6“We also identified the transcription factor LbMYB48 as an upstream regulator of LbRZF1 transcription”
Claim · model-written summary
Heterologous expression of LbRZF1 in Arabidopsis thaliana conferred salt tolerance.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae7 - Treatment
- heterologous expression of LbRZF1e7
- Gene
- LbRZF1e7
- Outcome
- salt tolerancee7
- Direction
- conferrede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“The heterologous expression of LbRZF1 in Arabidopsis thaliana conferred salt tolerance to this non-halophyte”
The same questions in other papers
loss of function · tolerance: abiotic · Limonium bicolorPositive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
overexpression · tolerance: abiotic · Arabidopsis thaliana (thale cress)Positive and negative results
17 papers in the same direction as this paper · 3 in the opposite direction
- negative
- no effect
- negative
17 papers more in this group
See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.