Abstract
The NAC transcription factors (TFs) are among the largest TF families in plants and play essential roles in growth, development, and stress responses. However, few studies have investigated how NAC TFs regulate salt gland development of recretohalophytes. In this study, we identified LbNAC55, a TF whose expression is upregulated by salt, from Limonium bicolor (a typical recretohalophyte which serves as a model for studying development and salt secretion of plant salt glands). Overexpression of LbNAC55 in L. bicolor significantly increases salt gland density and the salt tolerance of the species, whereas silencing LbNAC55 results in reduced salt gland development and diminished salt tolerance. Furthermore, yeast two-hybrid screening revealed that LbFLZ13 interacts with LbNAC55. Silencing LbFLZ13 similarly decreased salt gland formation and salt tolerance. Further studies showed that the reduction in salt gland density induced by the simultaneous silencing of both LbNAC55 and LbFLZ13 was significantly lower than that caused by the silencing of either gene alone. These results demonstrate that the regulatory modules of LbNAC55-LbFLZ13 control the expression of genes involved in salt gland development and secretion. These findings provide a new perspective on salt gland development in halophytes.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
LbNAC55 was identified as a salt-upregulated transcription factor in Limonium bicolor.
- Taxon
- Limonium bicolorLimonium bicolore0
- Treatment
- salte0
- Gene
- LbNAC55e0
- Outcome
- expressione0
- Direction
- upregulatede0
Not reportedComparator
Verbatim
e0“we identified LbNAC55, a TF whose expression is upregulated by salt, from Limonium bicolor”
Claim · model-written summary
LbNAC55 overexpression significantly increased salt gland density and salt tolerance in L. bicolor.
- Taxon
- L. bicolorLimonium bicolore0
- Treatment
- Overexpression of LbNAC55e0
- Gene
- LbNAC55e0
- Outcome
- salt gland density and the salt tolerancee0
- Direction
- increasese0
Not reportedComparator
Verbatim
e0“Overexpression of LbNAC55 in L. bicolor significantly increases salt gland density and the salt tolerance of the species”
Claim · model-written summary
Silencing LbNAC55 reduced salt gland development and salt tolerance.
- Taxon
- Limonium bicolorLimonium bicolort0
- Treatment
- silencing LbNAC55e0
- Outcome
- salt gland developmente0
- Direction
- reducede0
Not reportedComparator
Verbatim
e0“silencing LbNAC55 results in reduced salt gland development and diminished salt tolerance”
t0“Limonium bicolor”
Claim · model-written summary
Silencing LbNAC55 diminished salt tolerance.
- Taxon
- Limonium bicolorLimonium bicolort0
- Treatment
- silencing LbNAC55e0
- Outcome
- salt tolerancee0
- Direction
- diminishede0
Not reportedComparator
Verbatim
e0“silencing LbNAC55 results in reduced salt gland development and diminished salt tolerance”
t0“Limonium bicolor”
Claim · model-written summary
Yeast two-hybrid screening revealed interaction between LbFLZ13 and LbNAC55.
- Taxon
- Limonium bicolorLimonium bicolort0
- Gene
- LbFLZ13e0
- Outcome
- interacts with LbNAC55e0
Not reportedDirection
Verbatim
e0“yeast two-hybrid screening revealed that LbFLZ13 interacts with LbNAC55”
t0“Limonium bicolor”
Claim · model-written summary
Silencing LbFLZ13 decreased salt gland formation and salt tolerance.
- Taxon
- Limonium bicolorLimonium bicolort0
- Treatment
- Silencing LbFLZ13e0
- Outcome
- salt gland formation and salt tolerancee0
- Direction
- decreasede0
Not reportedComparator
Verbatim
e0“Silencing LbFLZ13 similarly decreased salt gland formation and salt tolerance”
t0“Limonium bicolor”
Claim · model-written summary
Simultaneous silencing of LbNAC55 and LbFLZ13 caused a significantly smaller reduction in salt gland density than silencing either alone.
Verbatim
e0“the reduction in salt gland density induced by the simultaneous silencing of both LbNAC55 and LbFLZ13 was significantly lower than that caused by the silencing of either gene alone”
t0“Limonium bicolor”
Claim · model-written summary
LbNAC55-LbFLZ13 regulatory modules control expression of genes involved in salt gland development and secretion.
- Taxon
- Limonium bicolorLimonium bicolort0
- Gene
- LbNAC55-LbFLZ13e0
- Outcome
- expression of genes involved in salt gland development and secretione0
Verbatim
e0“These results demonstrate that the regulatory modules of LbNAC55-LbFLZ13 control the expression of genes involved in salt gland development and secretion”
t0“Limonium bicolor”
The same questions in other papers
loss of function · growth · Limonium bicolorPositive and negative results
1 in the opposite direction
- positive
loss of function · tolerance: abiotic · Limonium bicolorPositive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.