Abstract
Mongolian oak (Quercus mongolica) is a common building material and landscaping tree species in northern China, with significant economic and ecological value. Its seedling growth is inhibited by high light intensity, but the mechanism by which light stress affects the growth and development of its seedlings remains unclear. In this study, we investigated the phenotypes, physiological processes, and molecular responses of 3-year-old Mongolian oak seedlings under different light treatments: full light (Sck), light shading (S1; 40% light), moderate shading (S2; 20% light), and severe shading (S3; 3% light). Compared to Sck, the S1 and S2 treatments resulted in higher leaf area, photosynthetic pigment content, photosynthesis rates, soluble sugar contents, and soluble protein contents in Mongolian oak seedlings. The S1 and S2 treatments also promoted seedling height and diameter growth and resulted in lower degrees of membrane lipid peroxidation, cell membrane permeability, and antioxidant enzyme activity. In contrast, severe shading (S3) significantly inhibited seedling height and diameter growth due to the lower net photosynthetic rate, and exhibiting higher degrees of membrane lipid peroxidation and cell membrane permeability. Shading treatments (S1 and S2) alleviated the negative effects of strong light on the growth and development of Mongolian oak seedlings, with the S2 treatment having the greatest effect. However, severe shading (S3) inhibited growth and development. A total of 3726 differentially expressed genes (DEGs) were detected in leaves under different shading treatments in RNA sequencing analysis. Among these, 1691, 3150, and 824 DEGs were detected in the Sck-S1, Sck-S2, and S1-S2 comparison groups, respectively. The different shading treatments determined common expression regulation pathways, including carotenoid biosynthesis, photosynthetic antenna proteins, and mitogen activated protein kinase (MAPK) signal transduction. Shading induced increases in gene expression levels in light harvesting complexes, which are related to changes in gene expression in the photosynthetic system, leading to changes in photosynthetic physiology. The expression levels of genes related to reactive oxygen species signal perception and activation enzymes were upregulated in Sck. Together, these findings revealed the response mechanisms of Mongolian oak seedlings to different shading levels at the physiological and molecular levels, providing a scientific basis and technical support for the cultivation and large-scale production of Mongolian oak seedlings.
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Claims 14
Claim · model-written summary
Light and moderate shading increased leaf area, photosynthetic pigment content, photosynthesis rates relative to full light.
- Taxon
- Mongolian oak
_in_December_2009.jpg?width=60)
Quercus mongolicae0 - Treatment
- S1 and S2 treatmentse0
- Outcome
- leaf area, photosynthetic pigment content, photosynthesis ratese0
- Direction
- highere0
- Comparator
- Scke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_in_December_2009.jpg?width=500)
Verbatim
e0“Compared to Sck, the S1 and S2 treatments resulted in higher leaf area, photosynthetic pigment content, photosynthesis rates, soluble sugar contents, and soluble protein contents in Mongolian oak seedlings”
Claim · model-written summary
Light and moderate shading increased soluble sugar contents, and soluble protein contents relative to full light.
- Taxon
- Mongolian oakQuercus mongolicae0
- Treatment
- S1 and S2 treatmentse0
- Outcome
- soluble sugar contents, and soluble protein contentse0
- Direction
- highere0
- Comparator
- Scke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to Sck, the S1 and S2 treatments resulted in higher leaf area, photosynthetic pigment content, photosynthesis rates, soluble sugar contents, and soluble protein contents in Mongolian oak seedlings”
Claim · model-written summary
S1 and S2 increased seedling height and diameter growth.
- Taxon
- Mongolian oakQuercus mongolicat0
- Treatment
- S1 and S2 treatmentse0
- Outcome
- seedling height and diameter growthe0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The S1 and S2 treatments also promoted seedling height and diameter growth”
t0“Mongolian oak”
Claim · model-written summary
S1 and S2 reduced membrane lipid peroxidation, permeability and antioxidant enzyme activity.
- Taxon
- Mongolian oakQuercus mongolicat0
- Treatment
- S1 and S2 treatmentse0
- Outcome
- membrane lipid peroxidation, cell membrane permeability, and antioxidant enzyme activitye0
- Direction
- lowere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“S1 and S2 treatments also promoted seedling height and diameter growth and resulted in lower degrees of membrane lipid peroxidation, cell membrane permeability, and antioxidant enzyme activity”
t0“Mongolian oak”
Claim · model-written summary
Severe shading significantly inhibited height and diameter growth.
- Taxon
- Mongolian oakQuercus mongolicat0
- Treatment
- severe shading (S3)e0
- Outcome
- seedling height and diameter growthe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“severe shading (S3) significantly inhibited seedling height and diameter growth”
t0“Mongolian oak”
Claim · model-written summary
Severe shading lowered net photosynthetic rate.
- Taxon
- Mongolian oakQuercus mongolicat0
- Treatment
- severe shading (S3)e0
- Outcome
- net photosynthetic ratee0
- Direction
- lowere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“severe shading (S3) significantly inhibited seedling height and diameter growth due to the lower net photosynthetic rate”
t0“Mongolian oak”
Claim · model-written summary
Severe shading increased membrane lipid peroxidation and permeability.
- Taxon
- Mongolian oakQuercus mongolicat0
- Treatment
- severe shading (S3)e0
- Outcome
- membrane lipid peroxidation and cell membrane permeabilitye0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“severe shading (S3) significantly inhibited seedling height and diameter growth due to the lower net photosynthetic rate, and exhibiting higher degrees of membrane lipid peroxidation and cell membrane permeability”
t0“Mongolian oak”
Claim · model-written summary
Moderate shading had the greatest alleviating effect on strong light stress.
- Taxon
- Mongolian oakQuercus mongolicae0
- Treatment
- S2 treatmente0
- Outcome
- greatest effecte0
- Direction
- greateste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shading treatments (S1 and S2) alleviated the negative effects of strong light on the growth and development of Mongolian oak seedlings, with the S2 treatment having the greatest effect”
Claim · model-written summary
Sck-S1 comparison yielded 1691 differentially expressed genes.
- Taxon
- Mongolian oakQuercus mongolicat0
- Outcome
- DEGse0
- Value
- 1691e0
- Comparator
- Sck-S1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among these, 1691, 3150, and 824 DEGs were detected in the Sck-S1, Sck-S2, and S1-S2 comparison groups, respectively”
t0“Mongolian oak”
Claim · model-written summary
Sck-S2 comparison yielded 3150 differentially expressed genes.
- Taxon
- Mongolian oakQuercus mongolicat0
- Outcome
- DEGse0
- Value
- 3150e0
- Comparator
- Sck-S2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among these, 1691, 3150, and 824 DEGs were detected in the Sck-S1, Sck-S2, and S1-S2 comparison groups, respectively”
t0“Mongolian oak”
Claim · model-written summary
S1-S2 comparison yielded 824 differentially expressed genes.
- Taxon
- Mongolian oakQuercus mongolicat0
- Outcome
- DEGse0
- Value
- 824e0
- Comparator
- S1-S2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among these, 1691, 3150, and 824 DEGs were detected in the Sck-S1, Sck-S2, and S1-S2 comparison groups, respectively”
t0“Mongolian oak”
Claim · model-written summary
Shading regulated carotenoid biosynthesis, antenna protein and MAPK pathways.
- Taxon
- Mongolian oakQuercus mongolicat0
- Treatment
- different shading treatmentse0
- Outcome
- carotenoid biosynthesis, photosynthetic antenna proteins, and mitogen activated protein kinase (MAPK) signal transductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The different shading treatments determined common expression regulation pathways, including carotenoid biosynthesis, photosynthetic antenna proteins, and mitogen activated protein kinase (MAPK) signal transduction”
t0“Mongolian oak”
Claim · model-written summary
Shading increased light harvesting complex gene expression.
- Taxon
- Mongolian oakQuercus mongolicat0
- Treatment
- Shadinge0
- Outcome
- gene expression levels in light harvesting complexese0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shading induced increases in gene expression levels in light harvesting complexes”
t0“Mongolian oak”
Claim · model-written summary
Genes for reactive oxygen species perception and activation enzymes were upregulated in full light.
- Taxon
- Mongolian oakQuercus mongolicat0
- Treatment
- Scke0
- Outcome
- expression levels of genes related to reactive oxygen species signal perception and activation enzymese0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression levels of genes related to reactive oxygen species signal perception and activation enzymes were upregulated in Sck”
t0“Mongolian oak”
The same questions in other papers
shade · physiology: photosynthesis · Quercus mongolica (Mongolian oak)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.