Abstract
Drought stress is a major abiotic stress that poses serious threats to the yield and quality of rapeseed. Understanding the mechanisms underlying the drought tolerance of rapeseed is of great importance for the breeding of drought-resistant rapeseed varieties. Here, we compared the phenotypic characteristics, photosynthetic physiology, specific metabolites and related metabolic pathways between the drought-tolerant genotype (Q2) and drought-sensitive genotype (Q8) of rapeseed under drought stress using gas chromatography and liquid chromatography–mass spectrometry. The results indicated that drought stress decreased the total biomass and photosynthesis of both genotypes, particularly those of Q8, and the reduction was substantially lower in the root surface area, root volume and root diameter of Q2 than in those of Q8 under drought stress. The adverse effect on Q2 could be improved by increasing the specific leaf area, root length, and non-photochemical quenching. Furthermore, four metabolic pathways (galactose metabolism, TCA cycle, aminoacyl-tRNA biosynthesis as well as nicotinate and nicotinamide metabolisms) were greatly activated in Q2 compared with those in Q8 under drought stress. Metabolic profiling revealed 17 differential metabolites between the two genotypes, including carbohydrates, amino acids and organic acids and the increase in tagatose was accompanied by the accumulation of fumaric acid in the roots of Q2 under drought stress. The drought resistance of Q2 could be attributed to the accumulation of more metabolites, such as galactose, tagatose, glycerone and fumaric acid. Our results provide valuable insights into the growth characteristics, physiological changes and metabolic regulation mechanism of rapeseed under drought stress as well as a theoretical basis for the breeding of drought-resistant rapeseed varieties.
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Claims 8
Claim · model-written summary
Drought stress decreased total biomass and photosynthesis of both rapeseed genotypes, particularly those of Q8.
- Taxon
- rapeseedt0
- Treatment
- drought stresse1
- Outcome
- total biomass and photosynthesise1
- Direction
- decreasede1
- Qualifier
- particularly those of Q8e1
Not reportedComparator
Verbatim
e1“drought stress decreased the total biomass and photosynthesis of both genotypes, particularly those of Q8”
t0“Comparative metabolomics analysis of tolerant and sensitive genotypes of rapeseed (Brassica napus L.) seedlings under drought stress”
Claim · model-written summary
Under drought, reductions in root surface area, root volume and root diameter were substantially lower in Q2 than in Q8.
Verbatim
e2“the reduction was substantially lower in the root surface area, root volume and root diameter of Q2 than in those of Q8 under drought stress”
t0“Comparative metabolomics analysis of tolerant and sensitive genotypes of rapeseed (Brassica napus L.) seedlings under drought stress”
Claim · model-written summary
Increasing specific leaf area, root length and non-photochemical quenching could improve the adverse drought effect on Q2.
Verbatim
e3“The adverse effect on Q2 could be improved by increasing the specific leaf area, root length, and non-photochemical quenching”
t0“Comparative metabolomics analysis of tolerant and sensitive genotypes of rapeseed (Brassica napus L.) seedlings under drought stress”
Claim · model-written summary
Galactose metabolism, TCA cycle and aminoacyl-tRNA biosynthesis were more activated in Q2 than in Q8 under drought stress.
Verbatim
e4“four metabolic pathways (galactose metabolism, TCA cycle, aminoacyl-tRNA biosynthesis as well as nicotinate and nicotinamide metabolisms) were greatly activated in Q2 compared with those in Q8 under drought stress”
t0“Comparative metabolomics analysis of tolerant and sensitive genotypes of rapeseed (Brassica napus L.) seedlings under drought stress”
Claim · model-written summary
Nicotinate and nicotinamide metabolisms were more activated in Q2 than in Q8 under drought stress.
Verbatim
e4“four metabolic pathways (galactose metabolism, TCA cycle, aminoacyl-tRNA biosynthesis as well as nicotinate and nicotinamide metabolisms) were greatly activated in Q2 compared with those in Q8 under drought stress”
t0“Comparative metabolomics analysis of tolerant and sensitive genotypes of rapeseed (Brassica napus L.) seedlings under drought stress”
Claim · model-written summary
Metabolic profiling revealed 17 differential metabolites between the two genotypes, including carbohydrates, amino acids and organic acids.
Verbatim
e5“Metabolic profiling revealed 17 differential metabolites between the two genotypes, including carbohydrates, amino acids and organic acids”
t0“Comparative metabolomics analysis of tolerant and sensitive genotypes of rapeseed (Brassica napus L.) seedlings under drought stress”
Claim · model-written summary
In Q2 roots under drought, the increase in tagatose was accompanied by an accumulation of fumaric acid.
Verbatim
e6“the increase in tagatose was accompanied by the accumulation of fumaric acid in the roots of Q2 under drought stress”
t0“Comparative metabolomics analysis of tolerant and sensitive genotypes of rapeseed (Brassica napus L.) seedlings under drought stress”
Claim · model-written summary
The drought resistance of Q2 may be attributed to accumulation of galactose, tagatose, glycerone and fumaric acid.
Verbatim
e7“The drought resistance of Q2 could be attributed to the accumulation of more metabolites, such as galactose, tagatose, glycerone and fumaric acid”
t0“Comparative metabolomics analysis of tolerant and sensitive genotypes of rapeseed (Brassica napus L.) seedlings under drought stress”
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