Abstract
Drought stress is a major limiting factor for canola production in arid and semi-arid regions, particularly during seed germination, seedling and flowering stages. In this study, we evaluated drought responses of doubled haploid (DH) lines derived from interspecific hybrids of B. napus × B. rapa and their parental cultivars under simulated (PEG-6000) and soil-based drought conditions. Drought stress significantly reduced germination, growth, and physiological performance in all genotypes; however, DH lines consistently exhibited superior tolerance. Under PEG-induced osmotic stress, DH lines maintained higher germination rates, root elongation, and relative water content compared with parental genotypes. During seedling and flowering stages drought, DH lines showed lower accumulation of hydrogen peroxide and malondialdehyde, alongside markedly higher antioxidant enzyme activities (CAT and POD) and improved photosynthetic efficiency (Fv/Fm). Gene expression analysis revealed strong induction of drought-responsive genes, including WRKY28, MYB, LTP, WSP, metallothionein, and protein kinase family genes, particularly in DH lines at prolonged stress exposure. Multivariate analyses (PCA and correlation) confirmed a close association between enhanced antioxidant capacity, transcriptional activation, and drought tolerance traits. Overall, our results demonstrate that homozygous doubled haploid lines derived from distant hybridization between B. napus and B. rapa exhibit enhanced drought tolerance at both early and reproductive stages. These genotypes represent valuable genetic resources for breeding drought-tolerance canola cultivars.
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Claims 10
Claim · model-written summary
Drought stress significantly reduced germination, growth and physiological performance in all canola genotypes.
- Taxon
- canola
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Brassica napus var. napuse0 - Treatment
- Drought stresse1
- Outcome
- germination, growth, and physiological performancee1
- Direction
- reducede1
- Qualifier
- in all genotypese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Drought stress is a major limiting factor for canola production in arid and semi-arid regions”
e1“Drought stress significantly reduced germination, growth, and physiological performance in all genotypes; however, DH lines consistently exhibited superior tolerance”
Claim · model-written summary
DH lines showed superior drought tolerance compared with their parental cultivars.
- Taxon
- canolaBrassica napus var. napuse0
- Material
- doubled haploid (DH) linese2
- Treatment
- Drought stresse1
- Outcome
- tolerancee1
- Direction
- superiore1
- Comparator
- their parental cultivarse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Drought stress is a major limiting factor for canola production in arid and semi-arid regions”
e1“Drought stress significantly reduced germination, growth, and physiological performance in all genotypes; however, DH lines consistently exhibited superior tolerance”
e2“doubled haploid (DH) lines derived from interspecific hybrids of B. napus × B. rapa and their parental cultivars”
Claim · model-written summary
Under PEG-induced osmotic stress, DH lines maintained higher germination rates, root elongation and relative water content than parental genotypes.
- Taxon
- canolaBrassica napus var. napuse0
- Material
- DH linese2
- Treatment
- PEG-induced osmotic stresse2
- Outcome
- germination rates, root elongation, and relative water contente2
- Direction
- highere2
- Comparator
- parental genotypese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Drought stress is a major limiting factor for canola production in arid and semi-arid regions”
e2“Under PEG-induced osmotic stress, DH lines maintained higher germination rates, root elongation, and relative water content compared with parental genotypes”
Claim · model-written summary
During seedling and flowering stage drought, DH lines accumulated less hydrogen peroxide and malondialdehyde.
- Taxon
- canolaBrassica napus var. napuse0
- Material
- DH linese3
- Organ / stage
- seedling and flowering stagese3
- Treatment
- droughte3
- Outcome
- accumulation of hydrogen peroxide and malondialdehydee3
- Direction
- lowere3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Drought stress is a major limiting factor for canola production in arid and semi-arid regions”
e3“During seedling and flowering stages drought, DH lines showed lower accumulation of hydrogen peroxide and malondialdehyde, alongside markedly higher antioxidant enzyme activities (CAT and POD) and improved photosynthetic efficiency (Fv/Fm)”
Claim · model-written summary
During seedling and flowering stage drought, DH lines had markedly higher antioxidant enzyme activities (CAT and POD).
- Taxon
- canolaBrassica napus var. napuse0
- Material
- DH linese3
- Organ / stage
- seedling and flowering stagese3
- Treatment
- droughte3
- Outcome
- antioxidant enzyme activities (CAT and POD)e3
- Direction
- markedly highere3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Drought stress is a major limiting factor for canola production in arid and semi-arid regions”
e3“During seedling and flowering stages drought, DH lines showed lower accumulation of hydrogen peroxide and malondialdehyde, alongside markedly higher antioxidant enzyme activities (CAT and POD) and improved photosynthetic efficiency (Fv/Fm)”
Claim · model-written summary
During seedling and flowering stage drought, DH lines had improved photosynthetic efficiency (Fv/Fm).
- Taxon
- canolaBrassica napus var. napuse0
- Material
- DH linese3
- Organ / stage
- seedling and flowering stagese3
- Treatment
- droughte3
- Outcome
- photosynthetic efficiency (Fv/Fm)e3
- Direction
- improvede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Drought stress is a major limiting factor for canola production in arid and semi-arid regions”
e3“During seedling and flowering stages drought, DH lines showed lower accumulation of hydrogen peroxide and malondialdehyde, alongside markedly higher antioxidant enzyme activities (CAT and POD) and improved photosynthetic efficiency (Fv/Fm)”
Claim · model-written summary
Drought-responsive genes including WRKY28, MYB, LTP, WSP, metallothionein and protein kinase family genes were strongly induced, particularly in DH lines under prolonged stress.
- Taxon
- canolaBrassica napus var. napuse0
- Material
- DH linese4
- Treatment
- prolonged stress exposuree4
- Gene
- WRKY28, MYB, LTP, WSP, metallothionein, and protein kinase family genese4
- Outcome
- drought-responsive genese4
- Direction
- inductione4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Drought stress is a major limiting factor for canola production in arid and semi-arid regions”
e4“Gene expression analysis revealed strong induction of drought-responsive genes, including WRKY28, MYB, LTP, WSP, metallothionein, and protein kinase family genes, particularly in DH lines at prolonged stress exposure”
Claim · model-written summary
Enhanced antioxidant capacity and transcriptional activation are closely associated with drought tolerance traits in canola.
- Taxon
- canolaBrassica napus var. napuse0
- Treatment
- droughte5
- Outcome
- drought tolerance traitse5
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Drought stress is a major limiting factor for canola production in arid and semi-arid regions”
e5“Multivariate analyses (PCA and correlation) confirmed a close association between enhanced antioxidant capacity, transcriptional activation, and drought tolerance traits”
Claim · model-written summary
Homozygous DH lines from B. napus x B. rapa show enhanced drought tolerance at early and reproductive stages.
- Taxon
- canolaBrassica napus var. napuse0
- Material
- homozygous doubled haploid linese6
- Organ / stage
- early and reproductive stagese6
- Treatment
- droughte6
- Outcome
- drought tolerancee6
- Direction
- enhancede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Drought stress is a major limiting factor for canola production in arid and semi-arid regions”
e6“homozygous doubled haploid lines derived from distant hybridization between B. napus and B. rapa exhibit enhanced drought tolerance at both early and reproductive stages”
Claim · model-written summary
The DH genotypes are valuable genetic resources for breeding drought-tolerant canola cultivars.
- Taxon
- canolaBrassica napus var. napuse0
- Outcome
- valuable genetic resourcese7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Drought stress is a major limiting factor for canola production in arid and semi-arid regions”
e7“These genotypes represent valuable genetic resources for breeding drought-tolerance canola cultivars”
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