Abstract
The presence of sugar in plant tissue can lead to an increase in the osmotic pressure within cells, a decrease in the freezing point of plants, and protection against ice crystal damage to the tissue. Trehalose is closely related to sucrose , which comprises the largest proportion of sugar and has become a hot topic of research in recent years. Our previous studies have confirmed that a key trehalose synthesis gene , TaTPS11 , from the cold-resistant winter wheat DM1, could enhance the cold resistance of plants by increasing sugar content. However, the underlying mechanism behind this phenomenon remains unclear. In this study, we cloned TaTPS11-6D , edited TaTPS11-6D using CRISPR/Cas9 technology and transformed ‘Fielder’ to obtain T 2 generation plants. We screened out OE3-3 and OE8-7 lines with significantly higher cold resistance than that of ‘Fielder’ and Cri 4–3 edited lines with significantly lower cold resistance than that of ‘Fielder’. Low temperature storage limiting factors were measured for OE3-3, OE8-7 and Cri 4–3 treated at different temperatures.The results showed that TaTPS11-6D significantly increased the content of sugar in plants and the transfer of sugar from source to storage organs under cold conditions. The TaTPS11-6D significantly increased the levels of salicylic, jasmonic, and abscisic acids while also significantly decreasing the level of gibberellic acid . Our research improves the model of low temperature storage capacity limiting factor.
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Claims 7
Claim · model-written summary
OE3-3 had significantly higher cold resistance than Fielder.
- Taxon
- wheat
Triticum aestivumt0 - Material
- OE3-3e0
- Treatment
- OE3-3e0
- Outcome
- cold resistancee0
- Direction
- highere0
- Comparator
- ‘Fielder’e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OE3-3 and OE8-7 lines with significantly higher cold resistance than that of ‘Fielder’”
t0“wheat”
Claim · model-written summary
OE8-7 had significantly higher cold resistance than Fielder.
- Taxon
- wheatTriticum aestivumt0
- Material
- OE8-7e0
- Treatment
- OE8-7e0
- Outcome
- cold resistancee0
- Direction
- highere0
- Comparator
- ‘Fielder’e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OE3-3 and OE8-7 lines with significantly higher cold resistance than that of ‘Fielder’”
t0“wheat”
Claim · model-written summary
Cri 4–3 edited wheat had significantly lower cold resistance than Fielder.
- Taxon
- wheatTriticum aestivumt0
- Material
- Cri 4–3e0
- Treatment
- Cri 4–3 edited linese0
- Outcome
- cold resistancee0
- Direction
- lowere0
- Comparator
- ‘Fielder’e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cri 4–3 edited lines with significantly lower cold resistance than that of ‘Fielder’”
t0“wheat”
Claim · model-written summary
TaTPS11-6D significantly increased sugar content under cold conditions.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- TaTPS11-6De0
- Gene
- TaTPS11-6De0
- Outcome
- content of sugare0
- Direction
- increasede0
- Qualifier
- under cold conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaTPS11-6D significantly increased the content of sugar in plants and the transfer of sugar from source to storage organs under cold conditions”
t0“wheat”
Claim · model-written summary
TaTPS11-6D significantly increased sugar transfer from source to storage organs under cold conditions.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- TaTPS11-6De0
- Gene
- TaTPS11-6De0
- Outcome
- transfer of sugar from source to storage organse0
- Direction
- increasede0
- Qualifier
- under cold conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaTPS11-6D significantly increased the content of sugar in plants and the transfer of sugar from source to storage organs under cold conditions”
t0“wheat”
Claim · model-written summary
TaTPS11-6D significantly increased salicylic, jasmonic and abscisic acid levels.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- TaTPS11-6De0
- Gene
- TaTPS11-6De0
- Outcome
- levels of salicylic, jasmonic, and abscisic acidse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaTPS11-6D significantly increased the levels of salicylic, jasmonic, and abscisic acids”
t0“wheat”
Claim · model-written summary
TaTPS11-6D significantly decreased gibberellic acid level.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- TaTPS11-6De1
- Gene
- TaTPS11-6De1
- Outcome
- level of gibberellic acide1
- Direction
- decreasinge1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“TaTPS11-6D significantly increased the levels of salicylic, jasmonic, and abscisic acids while also significantly decreasing the level of gibberellic acid”
t0“wheat”
The same questions in other papers
loss of function · tolerance: abiotic · Triticum aestivum (wheat)Positive and negative results
4 papers in the same direction as this paper · 8 in the opposite direction
- positive
- positive
- positive
9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.