Abstract
BACKGROUND: As functional proteins, dehydrins are found in many maturing seeds and vegetable tissues under adverse environmental conditions. However, the regulation of dehydrin expression remains unclear. RESULTS: In this study, a novel drought stress-related bHLH transcription factor, TabHLH49, was isolated from a wheat cDNA library treated with the drought and cold stress by using yeast one-hybrid system. TabHLH49 protein possesses a typical conserved bHLH domain and is a Myc-type bHLH transcription factor. TabHLH49 was detected in the nucleus of tobacco epidermal cells, and the amino acid sequences at the C-terminus (amino acids 323-362) is necessary for its transactivation activity. Real-time PCR analyses revealed the tissue-specific expression and drought stress-responsive expression of TabHLH49 in wheat. In addition, the verification in Y1H and electrophoretic mobility shift assays illustrated that TabHLH49 protein can bind and interact with the promoter of the wheat WZY2 dehydrin. Furthermore, the dual-luciferase assays showed that TabHLH49 can positively regulate the expression of WZY2 dehydrin. The transient expression and BSMV-mediated gene silencing of TabHLH49 also showed that TabHLH49 positively regulates the expression of WZY2 dehydrin and improves drought stress resistance in wheat. CONCLUSIONS: These results provide direct evidences that TabHLH49 positively regulates expression level of dehydrin WZY2 gene and improves drought tolerance of wheat.
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Claims 8
Claim · model-written summary
TabHLH49 possesses a typical conserved bHLH domain and is a Myc-type bHLH transcription factor.
- Taxon
- wheat
Triticum aestivumt0 - Gene
- TabHLH49e1
- Outcome
- typical conserved bHLH domaine1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“enhances drought stress tolerance in wheat”
e1“TabHLH49 protein possesses a typical conserved bHLH domain and is a Myc-type bHLH transcription factor”
Claim · model-written summary
TabHLH49 is detected in the nucleus of tobacco epidermal cells.
- Taxon
- wheatTriticum aestivumt0
- Organ / stage
- epidermal cellse2
- Gene
- TabHLH49e2
- Outcome
- detected in the nucleuse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“enhances drought stress tolerance in wheat”
e2“TabHLH49 was detected in the nucleus of tobacco epidermal cells”
Claim · model-written summary
The C-terminus (amino acids 323-362) of TabHLH49 is necessary for its transactivation activity.
- Taxon
- wheatTriticum aestivumt0
- Gene
- C-terminus (amino acids 323-362)e3
- Outcome
- transactivation activitye3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“enhances drought stress tolerance in wheat”
e3“the amino acid sequences at the C-terminus (amino acids 323-362) is necessary for its transactivation activity”
Claim · model-written summary
TabHLH49 shows tissue-specific expression and drought stress-responsive expression in wheat.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- drought stresse4
- Gene
- TabHLH49e4
- Outcome
- tissue-specific expression and drought stress-responsive expression of TabHLH49e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“enhances drought stress tolerance in wheat”
e4“Real-time PCR analyses revealed the tissue-specific expression and drought stress-responsive expression of TabHLH49 in wheat”
Claim · model-written summary
TabHLH49 binds and interacts with the promoter of the wheat WZY2 dehydrin.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TabHLH49e5
- Outcome
- bind and interact with the promoter of the wheat WZY2 dehydrine5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“enhances drought stress tolerance in wheat”
e5“the verification in Y1H and electrophoretic mobility shift assays illustrated that TabHLH49 protein can bind and interact with the promoter of the wheat WZY2 dehydrin”
Claim · model-written summary
TabHLH49 positively regulates the expression of WZY2 dehydrin in dual-luciferase assays.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- TabHLH49e6
- Gene
- WZY2 dehydrine6
- Outcome
- the expression of WZY2 dehydrine6
- Direction
- positively regulatee6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“enhances drought stress tolerance in wheat”
e6“the dual-luciferase assays showed that TabHLH49 can positively regulate the expression of WZY2 dehydrin”
Claim · model-written summary
Transient expression and BSMV-mediated gene silencing of TabHLH49 show that TabHLH49 positively regulates the expression of WZY2 dehydrin in wheat.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- transient expression and BSMV-mediated gene silencing of TabHLH49e7
- Gene
- WZY2 dehydrine7
- Outcome
- the expression of WZY2 dehydrine7
- Direction
- positively regulatese7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“enhances drought stress tolerance in wheat”
e7“The transient expression and BSMV-mediated gene silencing of TabHLH49 also showed that TabHLH49 positively regulates the expression of WZY2 dehydrin”
Claim · model-written summary
Transient expression and BSMV-mediated gene silencing of TabHLH49 improve drought stress resistance in wheat.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- transient expression and BSMV-mediated gene silencing of TabHLH49e7
- Outcome
- drought stress resistancee7
- Direction
- improvese7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“enhances drought stress tolerance in wheat”
e7“The transient expression and BSMV-mediated gene silencing of TabHLH49 also showed that TabHLH49 positively regulates the expression of WZY2 dehydrin and improves drought stress resistance in wheat”
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