Abstract
Improving the drought resistance of rice is of great significance for expanding the planting area and improving the stable yield of rice. In our previous work, we found that ROLLED AND ERECT LEAF1 (REL1) protein promoted enhanced tolerance to drought stress by eliminating reactive oxygen species (ROS) levels and triggering the abscisic acid (ABA) response. However, the mechanism through which REL1 regulates drought tolerance by removing ROS is unclear. In this study, we identified REL1 interacting protein 5 (RIP5) and found that it directly combines with REL1 in the chloroplast. We found that RIP5 was strongly expressed in ZH11 under drought-stress conditions, and that the rip5-ko mutants significantly improved the tolerance of rice plants to drought, whereas overexpression of RIP5 resulted in greater susceptibility to drought. Further investigation suggested that RIP5 negatively regulated drought tolerance in rice by decreasing the content of ascorbic acid (AsA), thereby reducing ROS clearance. RNA sequencing showed that the knockout of RIP5 caused differential gene expression that is chiefly associated with ascorbate and aldarate metabolism. Furthermore, multiple experimental results suggest that REL1 is involved in regulating drought tolerance by inhibiting RIP5. Collectively, our findings reveal the importance of the inhibition of RIP5 by REL1 in affecting the rice’s response to drought stress. This work not only explains the drought tolerance mechanism of rice, but will also help to improve the drought tolerance of rice.
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Claims 7
Claim · model-written summary
RIP5 directly combines with REL1 in the chloroplast.
- Taxon
- Rice
Oryza sativat0 - Organ / stage
- chloroplaste1
- Gene
- RIP5e1
- Outcome
- directly combines with REL1e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Rice”
e1“we identified REL1 interacting protein 5 (RIP5) and found that it directly combines with REL1 in the chloroplast”
Claim · model-written summary
RIP5 was strongly expressed in ZH11 rice under drought-stress conditions.
- Taxon
- riceOryza sativae0
- Material
- ZH11e2
- Treatment
- drought-stress conditionse2
- Gene
- RIP5e2
- Outcome
- strongly expressede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Improving the drought resistance of rice”
e2“RIP5 was strongly expressed in ZH11 under drought-stress conditions”
Claim · model-written summary
The rip5-ko mutants significantly improved drought tolerance of rice plants compared with the tolerance of the unmodified plants.
- Taxon
- riceOryza sativae3
- Material
- rip5-ko mutantse3
- Treatment
- droughte3
- Outcome
- tolerancee3
- Direction
- improvede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Improving the drought resistance of rice”
e3“the rip5-ko mutants significantly improved the tolerance of rice plants to drought”
Claim · model-written summary
Overexpression of RIP5 resulted in greater susceptibility to drought in rice.
- Taxon
- riceOryza sativae0
- Treatment
- overexpression of RIP5e4
- Gene
- RIP5e4
- Outcome
- susceptibility to droughte4
- Direction
- greatere4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Improving the drought resistance of rice”
e4“overexpression of RIP5 resulted in greater susceptibility to drought”
Claim · model-written summary
RIP5 negatively regulates drought tolerance in rice by decreasing ascorbic acid content, thereby reducing ROS clearance.
- Taxon
- riceOryza sativae5
- Gene
- RIP5e5
- Compound
- ascorbic acid (AsA)e5
- Outcome
- drought tolerancee5
- Qualifier
- thereby reducing ROS clearancee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Improving the drought resistance of rice”
e5“Further investigation suggested that RIP5 negatively regulated drought tolerance in rice by decreasing the content of ascorbic acid (AsA), thereby reducing ROS clearance”
Claim · model-written summary
Knockout of RIP5 caused differential gene expression in rice chiefly associated with ascorbate and aldarate metabolism.
- Taxon
- riceOryza sativae0
- Treatment
- knockout of RIP5e6
- Outcome
- differential gene expressione6
- Qualifier
- chiefly associated with ascorbate and aldarate metabolisme6
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Improving the drought resistance of rice”
e6“RNA sequencing showed that the knockout of RIP5 caused differential gene expression that is chiefly associated with ascorbate and aldarate metabolism”
Claim · model-written summary
REL1 regulates drought tolerance in rice by inhibiting RIP5.
- Taxon
- riceOryza sativae0
- Gene
- REL1e7
- Outcome
- drought tolerancee7
- Qualifier
- by inhibiting RIP5e7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Improving the drought resistance of rice”
e7“multiple experimental results suggest that REL1 is involved in regulating drought tolerance by inhibiting RIP5”