Abstract
In the present study, start codon targeted (SCoT) polymorphism and CAAT box-derived polymorphism (CBDP) molecular markers were used to study the genetic diversity of ten strawberry cultivars. In addition, shoot regeneration from in vitro leaf explants of ten commercial cultivars, i.e Aicyberry, Kayonoka, Tochiotome, Penchika, Amauo, Pajado, Selva, Monterey, Camarosa, and Albion, was also investigated. The result showed that a total of 9 SCoT primers and 9 CBDP primers detected genetic polymorphism among ten strawberry cultivars. Comparatively, SCoT markers proved to be more efficient than CBDP markers in terms of percentage polymorphism as the former detected an average of 74.49% and the latter at 71.15%. Meanwhile, the indices of genetic diversity from the aggregated data of two techniques SCoT and CBDP are as follows: expected heterozygosity (He) 0.2480, Shannon index (I) 0.3749, polymorphic band ratio 75.56%. The highest level of genetic similarity among the cultivars was 0.827 (between Aicyberry and Kayonoka varieties), the lowest was 0.586 (between Aicyberry and Selva varieties), and the average was 0.724. In addition, the results of shoot regeneration of strawberry varieties from leaf discs with different sizes were strongly dependent on the genotype and weakly on explant size. Leaf explants (3 mm in size) of Camarosa cultivar cultured on MS medium supplemented with 7 g/l agar, 30 g/l sucrose and 2 mg/l TDZ gave the best shoot regeneration.
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Claims 11
Claim · model-written summary
Nine SCoT and nine CBDP primers detected genetic polymorphism among ten strawberry cultivars.
- Taxon
- strawberry
Fragaria ×ananassae0 - Outcome
- genetic polymorphisme0
- Value
- 9 SCoT primers and 9 CBDP primerse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“a total of 9 SCoT primers and 9 CBDP primers detected genetic polymorphism among ten strawberry cultivars”
Claim · model-written summary
SCoT markers detected average polymorphism of 74.49%, more than CBDP markers.
- Taxon
- strawberry
Fragaria ×ananassat0 - Outcome
- percentage polymorphisme0
- Direction
- more efficiente0
- Value
- 74.49%e0
- Qualifier
- 71.15%e0
- Comparator
- CBDP markerse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“SCoT markers proved to be more efficient than CBDP markers in terms of percentage polymorphism as the former detected an average of 74.49% and the latter at 71.15%”
t0“strawberry”
Claim · model-written summary
Combined marker data gave expected heterozygosity of 0.2480.
- Taxon
- strawberry
Fragaria ×ananassat0 - Outcome
- expected heterozygositye0
- Value
- 0.2480e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“expected heterozygosity (He) 0.2480, Shannon index (I) 0.3749, polymorphic band ratio 75.56%”
t0“strawberry”
Claim · model-written summary
Combined marker data gave a Shannon index of 0.3749.
- Taxon
- strawberry
Fragaria ×ananassat0 - Outcome
- Shannon indexe0
- Value
- 0.3749e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“Shannon index (I) 0.3749, polymorphic band ratio 75.56%”
t0“strawberry”
Claim · model-written summary
Combined marker data gave a polymorphic band ratio of 75.56%.
- Taxon
- strawberry
Fragaria ×ananassat0 - Outcome
- polymorphic band ratioe0
- Value
- 75.56%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“polymorphic band ratio 75.56%”
t0“strawberry”
Claim · model-written summary
Genetic similarity was highest between Aicyberry and Kayonoka at 0.827.
- Taxon
- strawberry
Fragaria ×ananassat0 - Material
- Aicyberry and Kayonoka varietiese0
- Outcome
- genetic similaritye0
- Direction
- higheste0
- Value
- 0.827e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“The highest level of genetic similarity among the cultivars was 0.827 (between Aicyberry and Kayonoka varieties)”
t0“strawberry”
Claim · model-written summary
Genetic similarity was lowest between Aicyberry and Selva at 0.586.
- Taxon
- strawberry
Fragaria ×ananassat0 - Material
- Aicyberry and Selva varietiese0
- Outcome
- loweste0
- Direction
- loweste0
- Value
- 0.586e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“the lowest was 0.586 (between Aicyberry and Selva varieties)”
t0“strawberry”
Claim · model-written summary
Average genetic similarity was 0.724.
- Taxon
- strawberry
Fragaria ×ananassat0 - Outcome
- averagee0
- Value
- 0.724e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“and the average was 0.724”
t0“strawberry”
Claim · model-written summary
Strawberry shoot regeneration depended strongly on genotype.
- Taxon
- strawberry
Fragaria ×ananassae0 - Material
- genotypee0
- Organ / stage
- leaf discse0
- Outcome
- shoot regeneratione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“shoot regeneration of strawberry varieties from leaf discs with different sizes were strongly dependent on the genotype and weakly on explant size”
Claim · model-written summary
Strawberry shoot regeneration depended weakly on explant size.
- Taxon
- strawberry
Fragaria ×ananassae0 - Organ / stage
- leaf discse0
- Outcome
- shoot regeneratione0
- Qualifier
- explant sizee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“shoot regeneration of strawberry varieties from leaf discs with different sizes were strongly dependent on the genotype and weakly on explant size”
Claim · model-written summary
Three millimetre Camarosa leaf explants on supplemented MS medium gave the best shoot regeneration.
- Taxon
- strawberry
Fragaria ×ananassat0 - Material
- Camarosa cultivare0
- Organ / stage
- Leaf explantse0
- Treatment
- MS medium supplemented with 7 g/l agar, 30 g/l sucrose and 2 mg/l TDZe0
- Outcome
- shoot regeneratione0
- Direction
- beste0
- Qualifier
- 3 mm in sizee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“Leaf explants (3 mm in size) of Camarosa cultivar cultured on MS medium supplemented with 7 g/l agar, 30 g/l sucrose and 2 mg/l TDZ gave the best shoot regeneration”
t0“strawberry”
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