Paper · 2024 · Ministry of Science and Technology Vietnam

Genetic diversity analysis using molecular markers and in vitro shoot regeneration of strawberry cultivars in Lam Dong

Ba Nam Nguyen; Vu Mai Quynh Luong; Trinh Huy Tra Le; et al.

11
claims
19
verbatim quotes
1
citations
3
research areas
✓
Open access

Abstract

In the present study, start codon targeted (SCoT) polymorphism and CAAT box-derived polymorphism (CBDP) molecular markers were used to study the genetic diversity of ten strawberry cultivars. In addition, shoot regeneration from in vitro leaf explants of ten commercial cultivars, i.e Aicyberry, Kayonoka, Tochiotome, Penchika, Amauo, Pajado, Selva, Monterey, Camarosa, and Albion, was also investigated. The result showed that a total of 9 SCoT primers and 9 CBDP primers detected genetic polymorphism among ten strawberry cultivars. Comparatively, SCoT markers proved to be more efficient than CBDP markers in terms of percentage polymorphism as the former detected an average of 74.49% and the latter at 71.15%. Meanwhile, the indices of genetic diversity from the aggregated data of two techniques SCoT and CBDP are as follows: expected heterozygosity (He) 0.2480, Shannon index (I) 0.3749, polymorphic band ratio 75.56%. The highest level of genetic similarity among the cultivars was 0.827 (between Aicyberry and Kayonoka varieties), the lowest was 0.586 (between Aicyberry and Selva varieties), and the average was 0.724. In addition, the results of shoot regeneration of strawberry varieties from leaf discs with different sizes were strongly dependent on the genotype and weakly on explant size. Leaf explants (3 mm in size) of Camarosa cultivar cultured on MS medium supplemented with 7 g/l agar, 30 g/l sucrose and 2 mg/l TDZ gave the best shoot regeneration.

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Claims 11

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Nine SCoT and nine CBDP primers detected genetic polymorphism among ten strawberry cultivars.

Taxon
strawberryFragaria ×ananassae0
Outcome
genetic polymorphisme0
Value
9 SCoT primers and 9 CBDP primerse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“a total of 9 SCoT primers and 9 CBDP primers detected genetic polymorphism among ten strawberry cultivars”

✓ located verbatim in the source · abstract, characters 428–533 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

SCoT markers detected average polymorphism of 74.49%, more than CBDP markers.

Taxon
strawberryFragaria ×ananassat0
Outcome
percentage polymorphisme0
Direction
more efficiente0
Value
74.49%e0
Qualifier
71.15%e0
Comparator
CBDP markerse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“SCoT markers proved to be more efficient than CBDP markers in terms of percentage polymorphism as the former detected an average of 74.49% and the latter at 71.15%”

✓ located verbatim in the source · abstract, characters 550–713 · show in text ↑
t0

“strawberry”

✓ located verbatim in the source · title, characters 86–96 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Combined marker data gave expected heterozygosity of 0.2480.

Taxon
strawberryFragaria ×ananassat0
Outcome
expected heterozygositye0
Value
0.2480e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“expected heterozygosity (He) 0.2480, Shannon index (I) 0.3749, polymorphic band ratio 75.56%”

✓ located verbatim in the source · abstract, characters 832–924 · show in text ↑
t0

“strawberry”

✓ located verbatim in the source · title, characters 86–96 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Combined marker data gave a Shannon index of 0.3749.

Taxon
strawberryFragaria ×ananassat0
Outcome
Shannon indexe0
Value
0.3749e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Shannon index (I) 0.3749, polymorphic band ratio 75.56%”

✓ located verbatim in the source · abstract, characters 869–924 · show in text ↑
t0

“strawberry”

✓ located verbatim in the source · title, characters 86–96 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Combined marker data gave a polymorphic band ratio of 75.56%.

Taxon
strawberryFragaria ×ananassat0
Outcome
polymorphic band ratioe0
Value
75.56%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“polymorphic band ratio 75.56%”

✓ located verbatim in the source · abstract, characters 895–924 · show in text ↑
t0

“strawberry”

✓ located verbatim in the source · title, characters 86–96 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Genetic similarity was highest between Aicyberry and Kayonoka at 0.827.

Taxon
strawberryFragaria ×ananassat0
Material
Aicyberry and Kayonoka varietiese0
Outcome
genetic similaritye0
Direction
higheste0
Value
0.827e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The highest level of genetic similarity among the cultivars was 0.827 (between Aicyberry and Kayonoka varieties)”

✓ located verbatim in the source · abstract, characters 926–1038 · show in text ↑
t0

“strawberry”

✓ located verbatim in the source · title, characters 86–96 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Genetic similarity was lowest between Aicyberry and Selva at 0.586.

Taxon
strawberryFragaria ×ananassat0
Material
Aicyberry and Selva varietiese0
Outcome
loweste0
Direction
loweste0
Value
0.586e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the lowest was 0.586 (between Aicyberry and Selva varieties)”

✓ located verbatim in the source · abstract, characters 1040–1100 · show in text ↑
t0

“strawberry”

✓ located verbatim in the source · title, characters 86–96 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Average genetic similarity was 0.724.

Taxon
strawberryFragaria ×ananassat0
Outcome
averagee0
Value
0.724e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“and the average was 0.724”

✓ located verbatim in the source · abstract, characters 1102–1127 · show in text ↑
t0

“strawberry”

✓ located verbatim in the source · title, characters 86–96 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Strawberry shoot regeneration depended strongly on genotype.

Taxon
strawberryFragaria ×ananassae0
Material
genotypee0
Organ / stage
leaf discse0
Outcome
shoot regeneratione0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“shoot regeneration of strawberry varieties from leaf discs with different sizes were strongly dependent on the genotype and weakly on explant size”

✓ located verbatim in the source · abstract, characters 1157–1303 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Strawberry shoot regeneration depended weakly on explant size.

Taxon
strawberryFragaria ×ananassae0
Organ / stage
leaf discse0
Outcome
shoot regeneratione0
Qualifier
explant sizee0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“shoot regeneration of strawberry varieties from leaf discs with different sizes were strongly dependent on the genotype and weakly on explant size”

✓ located verbatim in the source · abstract, characters 1157–1303 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Three millimetre Camarosa leaf explants on supplemented MS medium gave the best shoot regeneration.

Taxon
strawberryFragaria ×ananassat0
Material
Camarosa cultivare0
Organ / stage
Leaf explantse0
Treatment
MS medium supplemented with 7 g/l agar, 30 g/l sucrose and 2 mg/l TDZe0
Outcome
shoot regeneratione0
Direction
beste0
Qualifier
3 mm in sizee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Leaf explants (3 mm in size) of Camarosa cultivar cultured on MS medium supplemented with 7 g/l agar, 30 g/l sucrose and 2 mg/l TDZ gave the best shoot regeneration”

✓ located verbatim in the source · abstract, characters 1305–1469 · show in text ↑
t0

“strawberry”

✓ located verbatim in the source · title, characters 86–96 · show in text ↑

sucrose · growth · Fragaria x ananassa (strawberry)Positive and negative results

12 papers: 10 positive, 2 negative or no effect

9 papers in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. negative
  3. positive

8 papers more in this group

See all 12 papers →

Thidiazuron · growth · Fragaria x ananassa (strawberry)Positive and negative results

21 papers: 20 positive, 1 negative or no effect

19 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive

17 papers more in this group

See all 21 papers →

agar · growth · Fragaria x ananassa (strawberry)Results agree

6 papers: 6 positive, 0 negative or no effect

5 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

2 papers more in this group

See all 6 papers →

in vitro · growth · Fragaria x ananassa (strawberry)Positive and negative results

86 papers: 76 positive, 10 negative or no effect

75 papers in the same direction as this paper · 9 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. no effect
  3. negative

82 papers more in this group

See all 86 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.