Abstract
Background Zinc (Zn) is a vital micronutrient essential for plant growth and development. Transporter proteins of the ZRT/IRT-like protein (ZIP) family play crucial roles in maintaining Zn homeostasis. Although the acquisition, translocation, and intracellular transport of Zn are well understood in plant roots and leaves, the genes that regulate these pathways in fruits remain largely unexplored. In this study, we aimed to investigate the function of SlZIP11 in regulating tomato fruit development. Methods We used Solanum lycopersicum L. ‘Micro-Tom’ SlZIP11 ( Solanum lycopersicum ) is highly expressed in tomato fruit, particularly in mature green (MG) stages. For obtaining results, we employed reverse transcription-quantitative polymerase chain reaction (RT-qPCR), yeast two-hybrid assay, bimolecular fluorescent complementation, subcellular localization assay, virus-induced gene silencing (VIGS), SlZIP11 overexpression, determination of Zn content, sugar extraction and content determination, and statistical analysis. Results RT-qPCR analysis showed elevated SlZIP11 expression in MG tomato fruits. SlZIP11 expression was inhibited and induced by Zn deficiency and toxicity treatments, respectively. Silencing SlZIP11 via the VIGS technology resulted in a significant increase in the Zn content of tomato fruits. In contrast, overexpression of SlZIP11 led to reduced Zn content in MG fruits. Moreover, both silencing and overexpression of SlZIP11 caused alterations in the fructose and glucose contents of tomato fruits. Additionally, SlSWEEET7a interacted with SlZIP11. The heterodimerization between SlSWEET7a and SlZIP11 affected subcellular targeting, thereby increasing the amount of intracellularly localized oligomeric complexes. Overall, this study elucidates the role of SlZIP11 in mediating Zn accumulation and sugar transport during tomato fruit ripening. These findings underscore the significance of SlZIP11 in regulating Zn levels and sugar content, providing insights into its potential implications for plant physiology and agricultural practices.
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Claims 9
Claim · model-written summary
SlZIP11 expression was elevated in mature green tomato fruit.
- Taxon
- tomato
Solanum lycopersicume0 - Organ / stage
- MG tomato fruitse0
- Gene
- SlZIP11e0
- Outcome
- SlZIP11 expressione0
- Direction
- elevatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“elevated SlZIP11 expression in MG tomato fruits”
Claim · model-written summary
Zinc deficiency inhibited SlZIP11 expression.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Zn deficiencye0
- Gene
- SlZIP11e0
- Outcome
- SlZIP11 expressione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlZIP11 expression was inhibited and induced by Zn deficiency and toxicity treatments, respectively”
t0“tomato”
Claim · model-written summary
Zinc toxicity induced SlZIP11 expression.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- toxicity treatmentse0
- Gene
- SlZIP11e0
- Outcome
- SlZIP11 expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlZIP11 expression was inhibited and induced by Zn deficiency and toxicity treatments, respectively”
t0“tomato”
Claim · model-written summary
SlZIP11 silencing significantly increased tomato fruit zinc content.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- Silencing SlZIP11 via the VIGS technologye0
- Gene
- SlZIP11e0
- Outcome
- Zn content of tomato fruitse0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing SlZIP11 via the VIGS technology resulted in a significant increase in the Zn content of tomato fruits”
Claim · model-written summary
SlZIP11 overexpression reduced zinc content in mature green fruit.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- overexpression of SlZIP11e0
- Gene
- SlZIP11e0
- Outcome
- Zn content in MG fruitse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of SlZIP11 led to reduced Zn content in MG fruits”
t0“tomato”
Claim · model-written summary
SlZIP11 silencing altered fructose and glucose content.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- silencinge0
- Gene
- SlZIP11e0
- Outcome
- fructose and glucose contents of tomato fruitse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both silencing and overexpression of SlZIP11 caused alterations in the fructose and glucose contents of tomato fruits”
Claim · model-written summary
SlZIP11 overexpression altered fructose and glucose content.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- overexpression of SlZIP11e0
- Gene
- SlZIP11e0
- Outcome
- fructose and glucose contents of tomato fruitse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both silencing and overexpression of SlZIP11 caused alterations in the fructose and glucose contents of tomato fruits”
Claim · model-written summary
SlSWEET7a interacted with SlZIP11.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- SlZIP11e0
- Outcome
- SlSWEEET7a interacted with SlZIP11e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlSWEEET7a interacted with SlZIP11”
t0“tomato”
Claim · model-written summary
SlSWEET7a and SlZIP11 heterodimerization increased intracellular oligomeric complexes by changing subcellular targeting.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- SlSWEET7a and SlZIP11e0
- Outcome
- intracellularly localized oligomeric complexese0
- Direction
- increasinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The heterodimerization between SlSWEET7a and SlZIP11 affected subcellular targeting, thereby increasing the amount of intracellularly localized oligomeric complexes”
t0“tomato”
The same questions in other papers
loss of function · nutrient: content · Solanum lycopersicum (tomato)Positive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- negative
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.