Abstract
Many plant roots acquire inorganic phosphate (Pi) from soils directly through the root-soil interface via high-affinity Pi transporters and/or through symbiotic associations between the cortical cells and arbuscular mycorrhizal fungi. In tomato, three phosphate transporters (LePT3, LePT4, and LePT5) are up-regulated upon colonization by arbuscular mycorrhizal fungi. In this study, the role of LePT4 in tomato is elucidated by molecular and physiological characterizations of a loss-of-function mutant lept4. In the absence of mycorrhizal infection and under solution-Pi concentrations (Cp) of 0.05 mM and 0.5 mM, the mutant exhibited severe Pi-deficiency symptoms which were associated with significantly lower Pi uptake as compared with that of the wild type. However, at a Cp of 5 mM, lept4 grew better than the wild type. Mycorrhizal infection at a Cp of 0.05 mM resulted in a significant increase in the transcripts of LePT4 in the wild type and a concomitant 2-fold increase in Pi uptake. Although upon mycorrhizal infection, lept4 also exhibited an increased Pi uptake, it was significantly lower than that of the wild type. Under a Cp of 1 mM and in the absence of mycorrhizal infection, LePT4 expression was suppressed in the wild type and a mutation in this gene resulted in a slight reduction in total Pi uptake. These data highlight the pivotal role of LePT4 in mycorrhizal-mediated Pi uptake in tomato, and show that this function may not be fully compensated by other members of the family. Characterization of the mycorrhiza-associated Pi transporter lept4 mutant, along with expression analysis of LePT3, provides evidence for the different routes of mycorrhiza-mediated Pi uptake in plants.
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Claims 9
Claim · model-written summary
LePT4 loss-of-function mutant had significantly lower Pi uptake than wild type without mycorrhiza at 0.05 and 0.5 mM solution Pi
- Taxon
- tomato
Solanum lycopersicumt0 - Material
- loss-of-function mutant lept4e2
- Treatment
- solution-Pi concentrations (Cp) of 0.05 mM and 0.5 mMe1
- Gene
- LePT4e2
- Outcome
- Pi uptakee1
- Direction
- lowere1
- Comparator
- wild typee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In the absence of mycorrhizal infection and under solution-Pi concentrations (Cp) of 0.05 mM and 0.5 mM, the mutant exhibited severe Pi-deficiency symptoms which were associated with significantly lower Pi uptake as compared with that of the wild type”
e2“the role of LePT4 in tomato is elucidated by molecular and physiological characterizations of a loss-of-function mutant lept4”
t0“a phosphate transporter in tomato”
Claim · model-written summary
At 5 mM solution Pi without mycorrhiza, the lept4 mutant grew better than wild type
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- lept4e3
- Treatment
- a Cp of 5 mMe3
- Outcome
- grewe3
- Direction
- bettere3
- Comparator
- wild typee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“However, at a Cp of 5 mM, lept4 grew better than the wild type”
t0“a phosphate transporter in tomato”
Claim · model-written summary
Mycorrhizal infection at 0.05 mM solution Pi significantly increased LePT4 transcripts in wild type tomato
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Mycorrhizal infection at a Cp of 0.05 mMe4
- Interactor
- arbuscular mycorrhizal fungie0symbiont
- Gene
- LePT4e4
- Outcome
- transcripts of LePT4e4
- Direction
- increasee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Mycorrhizal infection at a Cp of 0.05 mM resulted in a significant increase in the transcripts of LePT4 in the wild type and a concomitant 2-fold increase in Pi uptake”
e0“upon colonization by arbuscular mycorrhizal fungi”
t0“a phosphate transporter in tomato”
Claim · model-written summary
Mycorrhizal infection at 0.05 mM solution Pi increased Pi uptake 2-fold in tomato
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Mycorrhizal infection at a Cp of 0.05 mMe4
- Interactor
- arbuscular mycorrhizal fungie0symbiont
- Outcome
- Pi uptakee4
- Direction
- increasee4
- Value
- 2-folde4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Mycorrhizal infection at a Cp of 0.05 mM resulted in a significant increase in the transcripts of LePT4 in the wild type and a concomitant 2-fold increase in Pi uptake”
e0“upon colonization by arbuscular mycorrhizal fungi”
t0“a phosphate transporter in tomato”
Claim · model-written summary
Without mycorrhiza at 1 mM solution Pi, LePT4 expression was suppressed in wild type tomato
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- a Cp of 1 mMe5
- Gene
- LePT4e5
- Outcome
- LePT4 expressione5
- Direction
- suppressede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Under a Cp of 1 mM and in the absence of mycorrhizal infection, LePT4 expression was suppressed in the wild type and a mutation in this gene resulted in a slight reduction in total Pi uptake”
t0“a phosphate transporter in tomato”
Claim · model-written summary
LePT4 mutation slightly reduced total Pi uptake at 1 mM solution Pi without mycorrhiza
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- a mutation in this genee5
- Gene
- LePT4e5
- Outcome
- total Pi uptakee5
- Direction
- reductione5
- Qualifier
- slighte5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Under a Cp of 1 mM and in the absence of mycorrhizal infection, LePT4 expression was suppressed in the wild type and a mutation in this gene resulted in a slight reduction in total Pi uptake”
t0“a phosphate transporter in tomato”
Claim · model-written summary
LePT4 plays a pivotal role in mycorrhiza-mediated Pi uptake in tomato
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- LePT4e6
- Outcome
- mycorrhizal-mediated Pi uptakee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“These data highlight the pivotal role of LePT4 in mycorrhizal-mediated Pi uptake in tomato, and show that this function may not be fully compensated by other members of the family”
t0“a phosphate transporter in tomato”
Claim · model-written summary
The LePT4 function may not be fully compensated by other members of the transporter family
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- functione6
- Qualifier
- may not be fully compensated by other members of the familye6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“These data highlight the pivotal role of LePT4 in mycorrhizal-mediated Pi uptake in tomato, and show that this function may not be fully compensated by other members of the family”
t0“a phosphate transporter in tomato”
Claim · model-written summary
Expression analysis of LePT3 and the lept4 mutant provide evidence for different routes of mycorrhiza-mediated Pi uptake
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- LePT3e7
- Outcome
- routes of mycorrhiza-mediated Pi uptakee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Characterization of the mycorrhiza-associated Pi transporter lept4 mutant, along with expression analysis of LePT3, provides evidence for the different routes of mycorrhiza-mediated Pi uptake in plants”
t0“a phosphate transporter in tomato”
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43 papers in the same direction as this paper · 2 in the opposite direction · 1 reporting no difference from an alternative
- no effect
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See all 47 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.