Abstract
Auxins are a class of phytohormones with roles involved in the establishment and maintenance of the arbuscular mycorrhizal symbiosis (AMS). Auxin response factors (ARFs) and Auxin/Indole-acetic acids (AUX/IAAs), as two transcription factors of the auxin signaling pathway, coregulate the transcription of auxin response genes. However, the interrelation and regulatory mechanism of ARFs and AUX/IAAs in regulating AMS are still unclear. In this study, we found that the content of auxin in tomato roots increased sharply and revealed the importance of the auxin signaling pathway in the early stage of AMS. Notably, SlARF6 was found to play a negative role in AMF colonization. Silencing SlARF6 significantly increased the expression of AM-marker genes, as well as AMF-induced phosphorus uptake. SlIAA23 could interact with SlARF6 in vivo and in vitro, and promoted the AMS and phosphorus uptake. Interestingly, SlARF6 and SlIAA23 played a contrary role in strigolactone (SL) synthesis and accumulation in AMF-colonized roots of tomato plants. SlARF6 could directly bind to the AuxRE motif of the SlCCD8 promoter and inhibited its transcription, however, this effect was attenuated by SlIAA23 through interaction with SlARF6. Our results suggest that SlIAA23-SlARF6 coregulated tomato-AMS via an SL-dependent pathway, thus affecting phosphorus uptake in tomato plants.
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Claims 12
Claim · model-written summary
Auxin content rose sharply in tomato roots early in mycorrhizal symbiosis.
- Taxon
- tomato
Solanum lycopersicume0 - Organ / stage
- rootse0
- Outcome
- content of auxine0
- Direction
- increased sharplye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the content of auxin in tomato roots increased sharply”
Claim · model-written summary
SlARF6 negatively regulated AMF colonization.
- Taxon
- tomatoSolanum lycopersicumt0
- Interactor
- AMFe0symbiont
- Gene
- SlARF6e0
- Outcome
- AMF colonizatione0
- Qualifier
- negative rolee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlARF6 was found to play a negative role in AMF colonization.”
t0“tomato”
Claim · model-written summary
Silencing SlARF6 significantly increased AM marker gene expression.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Silencing SlARF6e0
- Gene
- SlARF6e0
- Outcome
- expression of AM-marker genese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing SlARF6 significantly increased the expression of AM-marker genes”
t0“tomato”
Claim · model-written summary
Silencing SlARF6 significantly increased AMF-induced phosphorus uptake.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Silencing SlARF6e0
- Interactor
- AMFe0symbiont
- Gene
- SlARF6e0
- Outcome
- AMF-induced phosphorus uptakee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing SlARF6 significantly increased the expression of AM-marker genes, as well as AMF-induced phosphorus uptake.”
t0“tomato”
Claim · model-written summary
SlIAA23 interacted with SlARF6 in vivo and in vitro.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- SlIAA23e0
- Outcome
- interact with SlARF6e0
- Qualifier
- in vivo and in vitroe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlIAA23 could interact with SlARF6 in vivo and in vitro”
t0“tomato”
Claim · model-written summary
SlIAA23 promoted mycorrhizal symbiosis.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlIAA23 could interact with SlARF6 in vivo and in vitro, and promoted the AMS and phosphorus uptake.”
t0“tomato”
Claim · model-written summary
SlIAA23 promoted phosphorus uptake.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- SlIAA23e0
- Outcome
- phosphorus uptakee0
- Direction
- promotede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlIAA23 could interact with SlARF6 in vivo and in vitro, and promoted the AMS and phosphorus uptake.”
t0“tomato”
Claim · model-written summary
SlARF6 and SlIAA23 had opposing roles in strigolactone synthesis and accumulation in AMF colonized roots.
- Taxon
- tomatoSolanum lycopersicume0
- Organ / stage
- rootse0
- Interactor
- AMFe0symbiont
- Gene
- SlARF6 and SlIAA23e0
- Outcome
- strigolactone (SL) synthesis and accumulatione0
- Direction
- contrarye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlARF6 and SlIAA23 played a contrary role in strigolactone (SL) synthesis and accumulation in AMF-colonized roots of tomato plants.”
Claim · model-written summary
SlARF6 bound the SlCCD8 promoter AuxRE motif.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- SlARF6e0
- Outcome
- bind to the AuxRE motif of the SlCCD8 promotere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlARF6 could directly bind to the AuxRE motif of the SlCCD8 promoter”
t0“tomato”
Claim · model-written summary
SlARF6 inhibited SlCCD8 transcription.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- SlARF6e0
- Outcome
- transcriptione0
- Direction
- inhibitede0
- Qualifier
- SlCCD8e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlARF6 could directly bind to the AuxRE motif of the SlCCD8 promoter and inhibited its transcription”
t0“tomato”
Claim · model-written summary
SlIAA23 attenuated the transcriptional inhibition through interaction with SlARF6.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- SlIAA23e0
- Outcome
- this effecte0
- Direction
- attenuatede0
- Qualifier
- interaction with SlARF6e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this effect was attenuated by SlIAA23 through interaction with SlARF6.”
t0“tomato”
Claim · model-written summary
The authors suggest SlIAA23-SlARF6 regulates tomato symbiosis via a strigolactone dependent pathway, affecting phosphorus uptake.
- Taxon
- tomatoSolanum lycopersicume0
- Gene
- SlIAA23-SlARF6e0
- Outcome
- phosphorus uptakee0
- Qualifier
- SL-dependent pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlIAA23-SlARF6 coregulated tomato-AMS via an SL-dependent pathway, thus affecting phosphorus uptake in tomato plants.”
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See all 80 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.