Abstract
In vitro studies of indica rice variety MR232 via somatic embryogenesis system was established by using mature seeds. By manipulating various plant growth regulators, the optimum medium was obtained by using MS medium containing 5 mg/L NAA (a-naphthaleneacetic acid) and 1 mg/L 2, 4-D (2,4-dichlorophenoxyacetic acid) without browning effect. This treatment showed higher percentage of callus induction and frequency of embryogenesis at the range of 91-97%, which was further, confirmed with the histology studies. The highest whitish somatic embryos frequency (87%) was initiated by incubating embryogenic calli on media containing 10 mg/L ABA and 9 mg/L gelrite agar for 4 weeks. However, the numbers of regenerated plant on medium containing NAA and that was previously pre-treated with 10 mg/L ABA, 9 mg/L gelrite agar and incubation at 8 weeks was the best treatment for shoots induction with 10 plantlets per 3 gm of somatic embryos.
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Claims 4
Claim · model-written summary
The MS medium with 5 mg/L NAA and 1 mg/L 2,4-D gave a higher percentage of callus induction and embryogenesis frequency, 91-97%, confirmed by histology, in indica rice MR232.
- Taxon
- indica rice
Oryza sativa Indica Groupe2 - Material
- MR232e2
- Organ / stage
- mature seedse2
- Treatment
- MS medium containing 5 mg/L NAA (a-naphthaleneacetic acid) and 1 mg/L 2, 4-D (2,4-dichlorophenoxyacetic acid)e0
- Outcome
- percentage of callus induction and frequency of embryogenesise1
- Direction
- highere1
- Value
- 91-97%e1
- Qualifier
- confirmed with the histology studiese1
- Setting
- In vitroe2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium containing 5 mg/L NAA (a-naphthaleneacetic acid) and 1 mg/L 2, 4-D (2,4-dichlorophenoxyacetic acid) without browning effect”
e1“This treatment showed higher percentage of callus induction and frequency of embryogenesis at the range of 91-97%, which was further, confirmed with the histology studies”
e2“In vitro studies of indica rice variety MR232 via somatic embryogenesis system was established by using mature seeds”
Claim · model-written summary
The MS medium with NAA and 2,4-D gave no browning effect in indica rice MR232.
- Taxon
- indica riceOryza sativa Indica Groupe2
- Material
- MR232e2
- Treatment
- MS medium containing 5 mg/L NAA (a-naphthaleneacetic acid) and 1 mg/L 2, 4-D (2,4-dichlorophenoxyacetic acid)e0
- Outcome
- browning effecte0
- Qualifier
- withoute0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium containing 5 mg/L NAA (a-naphthaleneacetic acid) and 1 mg/L 2, 4-D (2,4-dichlorophenoxyacetic acid) without browning effect”
e2“In vitro studies of indica rice variety MR232 via somatic embryogenesis system was established by using mature seeds”
Claim · model-written summary
Incubating embryogenic calli on media with 10 mg/L ABA and 9 mg/L gelrite agar for 4 weeks gave the highest whitish somatic embryo frequency, 87%, in indica rice.
- Taxon
- indica riceOryza sativa Indica Groupe2
- Organ / stage
- embryogenic callie3
- Treatment
- incubating embryogenic calli on media containing 10 mg/L ABA and 9 mg/L gelrite agar for 4 weekse3
- Outcome
- whitish somatic embryos frequencye3
- Direction
- higheste3
- Value
- 87%e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“In vitro studies of indica rice variety MR232 via somatic embryogenesis system was established by using mature seeds”
e3“The highest whitish somatic embryos frequency (87%) was initiated by incubating embryogenic calli on media containing 10 mg/L ABA and 9 mg/L gelrite agar for 4 weeks”
Claim · model-written summary
Shoot induction was best on NAA medium pre-treated with 10 mg/L ABA, 9 mg/L gelrite agar and 8 weeks of incubation, giving 10 plantlets per 3 gm of somatic embryos in indica rice.
- Taxon
- indica riceOryza sativa Indica Groupe2
- Treatment
- medium containing NAA and that was previously pre-treated with 10 mg/L ABA, 9 mg/L gelrite agar and incubation at 8 weekse4
- Outcome
- numbers of regenerated plante4
- Direction
- beste4
- Value
- 10 plantlets per 3 gm of somatic embryose4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“In vitro studies of indica rice variety MR232 via somatic embryogenesis system was established by using mature seeds”
e4“the numbers of regenerated plant on medium containing NAA and that was previously pre-treated with 10 mg/L ABA, 9 mg/L gelrite agar and incubation at 8 weeks was the best treatment for shoots induction with 10 plantlets per 3 gm of somatic embryos”
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