Abstract
Cold stress declines the quality and yield of tea, yet the molecular basis underlying cold tolerance of tea plants (Camellia sinensis) remains largely unknown. Here, we identified a circadian rhythm component LUX ARRHYTHMO (LUX) that potentially regulates cold tolerance of tea plants through a genome-wide association study and transcriptomic analysis. The expression of CsLUX phased with sunrise and sunset and was strongly induced by cold stress. Genetic assays indicated that CsLUX is a positive regulator of freezing tolerance in tea plants. CsLUX was directly activated by CsCBF1 and repressed the expression level of CsLOX2, which regulates the cold tolerance of tea plants through dynamically modulating jasmonic acid content. Furthermore, we showed that the CsLUX-CsJAZ1 complex attenuated the physical interaction of CsJAZ1 with CsICE1, liberating CsICE1 with transcriptional activities to withstand cold stress. Notably, a single-nucleotide variation of C-to-A in the coding region of CsLUX was functionally validated as the potential elite haplotype for cold response, which provided valuable molecular markers for future cold resistance breeding in tea plants.
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Claims 11
Claim · model-written summary
LUX ARRHYTHMO (LUX) potentially regulates cold tolerance in tea plants, identified through a genome-wide association study and transcriptomic analysis.
Verbatim
e0“we identified a circadian rhythm component LUX ARRHYTHMO (LUX) that potentially regulates cold tolerance of tea plants through a genome-wide association study and transcriptomic analysis”
Claim · model-written summary
The expression of CsLUX is phased with sunrise and sunset in tea plants.
Verbatim
e1“The expression of CsLUX phased with sunrise and sunset and was strongly induced by cold stress”
e2“cold tolerance of tea plants (Camellia sinensis)”
Claim · model-written summary
Cold stress strongly induces CsLUX expression in tea plants.
Not reportedComparator
Verbatim
e1“The expression of CsLUX phased with sunrise and sunset and was strongly induced by cold stress”
e2“cold tolerance of tea plants (Camellia sinensis)”
Claim · model-written summary
CsLUX is a positive regulator of freezing tolerance in tea plants, shown by genetic assays.
Verbatim
e3“Genetic assays indicated that CsLUX is a positive regulator of freezing tolerance in tea plants”
e2“cold tolerance of tea plants (Camellia sinensis)”
Claim · model-written summary
CsCBF1 directly activates CsLUX in tea plants.
Verbatim
e4“CsLUX was directly activated by CsCBF1 and repressed the expression level of CsLOX2, which regulates the cold tolerance of tea plants through dynamically modulating jasmonic acid content”
Claim · model-written summary
CsLUX represses the expression level of CsLOX2 in tea plants.
Verbatim
e4“CsLUX was directly activated by CsCBF1 and repressed the expression level of CsLOX2, which regulates the cold tolerance of tea plants through dynamically modulating jasmonic acid content”
Claim · model-written summary
CsLOX2 regulates cold tolerance of tea plants by modulating jasmonic acid content.
Verbatim
e4“CsLUX was directly activated by CsCBF1 and repressed the expression level of CsLOX2, which regulates the cold tolerance of tea plants through dynamically modulating jasmonic acid content”
Claim · model-written summary
CsLUX-CsJAZ1 complex attenuates the physical interaction of CsJAZ1 with CsICE1 in tea plants.
Verbatim
e5“the CsLUX-CsJAZ1 complex attenuated the physical interaction of CsJAZ1 with CsICE1, liberating CsICE1 with transcriptional activities to withstand cold stress”
e2“cold tolerance of tea plants (Camellia sinensis)”
Claim · model-written summary
Liberated CsICE1 retains transcriptional activity that helps tea plants withstand cold stress.
Verbatim
e5“the CsLUX-CsJAZ1 complex attenuated the physical interaction of CsJAZ1 with CsICE1, liberating CsICE1 with transcriptional activities to withstand cold stress”
e2“cold tolerance of tea plants (Camellia sinensis)”
Claim · model-written summary
A C-to-A single-nucleotide variation in the coding region of CsLUX is a potential elite haplotype for cold response in tea plants.
Verbatim
e6“a single-nucleotide variation of C-to-A in the coding region of CsLUX was functionally validated as the potential elite haplotype for cold response”
e2“cold tolerance of tea plants (Camellia sinensis)”
Claim · model-written summary
The CsLUX variant provides valuable molecular markers for future cold resistance breeding in tea plants.
Verbatim
e7“which provided valuable molecular markers for future cold resistance breeding in tea plants”
e2“cold tolerance of tea plants (Camellia sinensis)”
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