Abstract
Low-temperature stress is an increasing problem for the cultivation of tea (Camellia sinensis), with adverse effects on plant growth and development and subsequent negative impacts on the tea industry. Methyl jasmonate (MeJA), as a plant inducer, can improve the cold-stress tolerance in tea plants. R2R3-MYB transcription factors (TFs) are considered potentially important regulators in the resistance to cold stress in plants. However, the molecular mechanisms, by which MYB TFs via the jasmonic acid pathway respond to cold stress in the tea plant, remain unknown. In this study, physiological and biochemical assays showed that exogenous MeJA application could effectively promote ROS scavenging in the tea plant under cold stress, maintaining the stability of the cell membrane. Sixteen R2R3-MYB TFs genes were identified from the tea plant genome database. Quantitative RT-PCR analysis showed that three CsMYB genes were strongly induced under a combination of MeJA and cold-stress treatment. Subcellular localization assays suggest CsMYB45, CsMYB46, and CsMYB105 localized in the nucleus. Exogenous MeJA treatment enhanced the overexpression of CsMYB45, CsMYB46, and CsMYB105 in E. coli and improved the growth and survival rates of recombinant cells compared to an empty vector under cold stress. Yeast two-hybrid and bimolecular fluorescence complementation experiments confirmed that CsMYB46 and CsMYB105 interacted with CsJAZ3, CsJAZ10, and CsJAZ11 in the nucleus. Taken together, these results highlight that CsMYB45, CsMYB46, and CsMYB105 are not only key components in the cold-stress signal response pathway but also may serve as points of confluence for cold stress and JA signaling pathways. Furthermore, our findings provide new insight into how MYB TFs influence cold tolerance via the jasmonic acid pathway in tea and provide candidate genes for future functional studies and breeding.
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Claims 8
Claim · model-written summary
Methyl jasmonate promoted ROS scavenging and cell membrane stability in cold-stressed tea.
- Taxon
- tea plante0
- Treatment
- exogenous MeJA applicatione0
- Outcome
- ROS scavenginge0
- Direction
- promotee0
- Qualifier
- maintaining the stability of the cell membranee0
Not reportedComparator
Verbatim
e0“exogenous MeJA application could effectively promote ROS scavenging in the tea plant under cold stress, maintaining the stability of the cell membrane”
Claim · model-written summary
Sixteen R2R3-MYB transcription factor genes were identified in tea.
Verbatim
e0“Sixteen R2R3-MYB TFs genes were identified from the tea plant genome database”
Claim · model-written summary
Three CsMYB genes were strongly induced by methyl jasmonate plus cold stress.
- Taxon
- tea plante1
- Treatment
- combination of MeJA and cold-stress treatmente0
- Gene
- CsMYB genese0
- Outcome
- CsMYB genese0
- Direction
- strongly inducede0
Not reportedComparator
Verbatim
e0“three CsMYB genes were strongly induced under a combination of MeJA and cold-stress treatment”
e1“tea plant genome database”
Claim · model-written summary
CsMYB45, CsMYB46 and CsMYB105 localized in the nucleus.
Not reportedDirection
Verbatim
e0“CsMYB45, CsMYB46, and CsMYB105 localized in the nucleus”
e1“tea plant genome database”
Claim · model-written summary
Methyl jasmonate increased recombinant E. coli growth and survival under cold stress relative to empty vector.
- Taxon
- E. coli
.jpg?width=60)
Escherichia colie0 - Treatment
- Exogenous MeJA treatmente0
- Gene
- CsMYB45, CsMYB46, and CsMYB105e0
- Outcome
- growth and survival rates of recombinant cellse0
- Direction
- improvede0
- Comparator
- empty vectore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Exogenous MeJA treatment enhanced the overexpression of CsMYB45, CsMYB46, and CsMYB105 in E. coli and improved the growth and survival rates of recombinant cells compared to an empty vector under cold stress”
Claim · model-written summary
Methyl jasmonate enhanced CsMYB overexpression in E. coli.
- Taxon
- E. coliEscherichia colie0
- Treatment
- Exogenous MeJA treatmente0
- Outcome
- overexpression of CsMYB45, CsMYB46, and CsMYB105e0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous MeJA treatment enhanced the overexpression of CsMYB45, CsMYB46, and CsMYB105 in E. coli”
Claim · model-written summary
CsMYB46 and CsMYB105 interacted with CsJAZ3, CsJAZ10 and CsJAZ11 in the nucleus.
- Taxon
- tea plante1
- Gene
- CsMYB46 and CsMYB105e0
- Outcome
- interacted with CsJAZ3, CsJAZ10, and CsJAZ11 in the nucleuse0
Not reportedDirection
Verbatim
e0“CsMYB46 and CsMYB105 interacted with CsJAZ3, CsJAZ10, and CsJAZ11 in the nucleus”
e1“tea plant genome database”
Claim · model-written summary
CsMYB45, CsMYB46 and CsMYB105 may link cold and jasmonic acid signalling in tea.
Verbatim
e0“CsMYB45, CsMYB46, and CsMYB105 are not only key components in the cold-stress signal response pathway but also may serve as points of confluence for cold stress and JA signaling pathways”
e1“tea plant genome database”
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