Abstract
Alcohol dehydrogenase (ADH) is a vital enzyme in the biosynthesis pathway of six-carbon volatiles in plants. However, little is known about its functions in tea plants. Here, we identified two ADH genes (CsADH1 and CsADH2). An in vitro protein expression assay showed that both CsADH1 and CsADH2 proteins can catalyze the reduction of (Z)-3-hexenal into (Z)-3-hexenol. Subcellular localization revealed that both CsADH1 and CsADH2 proteins were predominantly localized in the nucleus and cytosol. CsADH1 had high transcripts in young stems in autumn, while CsADH2 showed extremely high expression levels in stems and roots. The expression of CsADH2 was mainly downregulated under ABA treatment, while CsADH1 and CsADH2 transcripts were significantly lower under MeJA treatment at 12 and 24 h. Under cold treatment, CsADH1 transcripts first decreased and then increased, while CsADH2 demonstrated an almost opposite expression pattern. Notably, CsADH2 was significantly upregulated under simulated Ectropis obliqua invasion. Gene suppression by antisense oligonucleotides (AsODNs) demonstrated that AsODN_ADH2 treatment significantly reduced CsADH2 transcripts and the abundance of (Z)-3-hexenol products. The results indicate that the two CsADH genes may play an important role in response to (a)biotic stresses and in the process of (Z)-3-hexenol biosynthesis.
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Claims 14
Claim · model-written summary
CsADH1 catalyzed reduction of (Z)-3-hexenal to (Z)-3-hexenol in vitro.
Verbatim
e0“both CsADH1 and CsADH2 proteins can catalyze the reduction of (Z)-3-hexenal into (Z)-3-hexenol”
t0“Tea Plants (Camellia sinensis)”
Claim · model-written summary
CsADH2 catalyzed reduction of (Z)-3-hexenal to (Z)-3-hexenol in vitro.
Verbatim
e0“both CsADH1 and CsADH2 proteins can catalyze the reduction of (Z)-3-hexenal into (Z)-3-hexenol”
t0“Tea Plants (Camellia sinensis)”
Claim · model-written summary
CsADH1 localized predominantly in the nucleus and cytosol.
Verbatim
e0“both CsADH1 and CsADH2 proteins were predominantly localized in the nucleus and cytosol”
t0“Tea Plants (Camellia sinensis)”
Claim · model-written summary
CsADH2 localized predominantly in the nucleus and cytosol.
Verbatim
e0“both CsADH1 and CsADH2 proteins were predominantly localized in the nucleus and cytosol”
t0“Tea Plants (Camellia sinensis)”
Claim · model-written summary
CsADH1 had high transcript levels in young stems in autumn.
Verbatim
e0“CsADH1 had high transcripts in young stems in autumn”
t0“Tea Plants (Camellia sinensis)”
Claim · model-written summary
CsADH2 had extremely high expression in stems and roots.
Verbatim
e0“CsADH2 showed extremely high expression levels in stems and roots”
t0“Tea Plants (Camellia sinensis)”
Claim · model-written summary
ABA treatment mainly downregulated CsADH2 expression.
- Taxon
- Tea Plantst0
- Treatment
- ABA treatmente0
- Gene
- CsADH2e0
- Outcome
- expression of CsADH2e0
- Direction
- downregulatede0
Not reportedComparator
Verbatim
e0“The expression of CsADH2 was mainly downregulated under ABA treatment”
t0“Tea Plants (Camellia sinensis)”
Claim · model-written summary
MeJA treatment significantly lowered CsADH1 transcripts at 12 and 24 h.
- Taxon
- Tea Plantst0
- Treatment
- MeJA treatmente0
- Gene
- CsADH1e0
- Outcome
- CsADH1 and CsADH2 transcriptse0
- Direction
- lowere0
- Qualifier
- 12 and 24 he0
Not reportedComparator
Verbatim
e0“CsADH1 and CsADH2 transcripts were significantly lower under MeJA treatment at 12 and 24 h”
t0“Tea Plants (Camellia sinensis)”
Claim · model-written summary
MeJA treatment significantly lowered CsADH2 transcripts at 12 and 24 h.
- Taxon
- Tea Plantst0
- Treatment
- MeJA treatmente0
- Gene
- CsADH2e0
- Outcome
- CsADH1 and CsADH2 transcriptse0
- Direction
- lowere0
- Qualifier
- 12 and 24 he0
Not reportedComparator
Verbatim
e0“CsADH1 and CsADH2 transcripts were significantly lower under MeJA treatment at 12 and 24 h”
t0“Tea Plants (Camellia sinensis)”
Claim · model-written summary
Under cold treatment CsADH1 transcripts first decreased and then increased.
- Taxon
- Tea Plantst0
- Treatment
- cold treatmente0
- Gene
- CsADH1e0
- Outcome
- CsADH1 transcriptse0
- Direction
- first decreased and then increasede0
Not reportedComparator
Verbatim
e0“Under cold treatment, CsADH1 transcripts first decreased and then increased”
t0“Tea Plants (Camellia sinensis)”
Claim · model-written summary
CsADH2 showed an almost opposite expression pattern under cold treatment.
- Taxon
- Tea Plantst0
- Treatment
- cold treatmente0
- Gene
- CsADH2e0
- Outcome
- CsADH2 demonstrated an almost opposite expression patterne0
Not reportedComparatorDirection
Verbatim
e0“Under cold treatment, CsADH1 transcripts first decreased and then increased, while CsADH2 demonstrated an almost opposite expression pattern”
t0“Tea Plants (Camellia sinensis)”
Claim · model-written summary
Simulated Ectropis obliqua invasion significantly upregulated CsADH2.
- Taxon
- Tea Plantst0
- Treatment
- simulated Ectropis obliqua invasione0
- Interactor
- Ectropis obliqua
Ectropis obliquae0pest - Gene
- CsADH2e0
- Outcome
- CsADH2e0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CsADH2 was significantly upregulated under simulated Ectropis obliqua invasion”
t0“Tea Plants (Camellia sinensis)”
Claim · model-written summary
AsODN_ADH2 significantly reduced CsADH2 transcripts.
- Taxon
- Tea Plantst0
- Treatment
- AsODN_ADH2 treatmente0
- Gene
- CsADH2e0
- Outcome
- CsADH2 transcriptse0
- Direction
- reducede0
Not reportedComparator
Verbatim
e0“AsODN_ADH2 treatment significantly reduced CsADH2 transcripts and the abundance of (Z)-3-hexenol products”
t0“Tea Plants (Camellia sinensis)”
Claim · model-written summary
AsODN_ADH2 significantly reduced abundance of (Z)-3-hexenol products.
- Taxon
- Tea Plantst0
- Treatment
- AsODN_ADH2 treatmente0
- Outcome
- abundance of (Z)-3-hexenol productse0
- Direction
- reducede0
Not reportedComparator
Verbatim
e0“AsODN_ADH2 treatment significantly reduced CsADH2 transcripts and the abundance of (Z)-3-hexenol products”
t0“Tea Plants (Camellia sinensis)”
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