Abstract
C-repeat (CRT)/dehydration responsive element (DRE)-binding factor CBFs, a small family of genes encoding transcriptional activators, play important roles in plant cold tolerance. In this study, a comprehensive genome-wide analysis was carried out to identify and characterize the functional dynamics of CsCBFs in tea plant (Camellia sinensis). A total of 6 CBF genes were obtained from the tea plant genome and named CBF1-6. All of the CsCBFs had an AP2/ERF DNA-binding domain and nuclear localization signal (NLS) sequence. CsCBF-eGFP fusion and DAPI staining analysis confirmed the nuclear localization of the CsCBFs. Transactivation assays showed that the CsCBFs, except CsCBF1, had transcriptional activity. CsCBF expression was differentially induced by cold, heat, PEG, salinity, ABA, GA, MeJA and SA stresses. In particular, the CsCBF genes were significantly induced by cold treatments. To further characterize the functions of CsCBF genes, we overexpressed the CsCBF3 gene in Arabidopsis thaliana plants. The resulting transgenic plants showed increased cold tolerance compared with the wild-type Arabidopsis plant. The enhanced cold tolerance of the transgenic plants was potentially achieved through an ABA-independent pathway. This study will help to increase our understanding of CsCBF genes and their contributions to stress tolerance in tea plants.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
Six CBF genes were identified in the tea genome.
Verbatim
e0“A total of 6 CBF genes were obtained from the tea plant genome and named CBF1-6.”
Claim · model-written summary
All CsCBFs have an AP2/ERF DNA-binding domain and NLS sequence.
Verbatim
e0“All of the CsCBFs had an AP2/ERF DNA-binding domain and nuclear localization signal (NLS) sequence.”
t0“tea plant (Camellia sinensis)”
Claim · model-written summary
CsCBFs localized to nuclei.
Verbatim
e0“CsCBF-eGFP fusion and DAPI staining analysis confirmed the nuclear localization of the CsCBFs.”
t0“tea plant (Camellia sinensis)”
Claim · model-written summary
CsCBFs except CsCBF1 showed transcriptional activity.
Verbatim
e0“Transactivation assays showed that the CsCBFs, except CsCBF1, had transcriptional activity.”
t0“tea plant (Camellia sinensis)”
Claim · model-written summary
CsCBF expression responded differently to multiple abiotic stresses and hormones.
- Taxon
- tea plantt0
- Treatment
- cold, heat, PEG, salinity, ABA, GA, MeJA and SA stressese0
- Gene
- CsCBFe0
- Outcome
- CsCBF expressione0
- Direction
- differentially inducede0
Not reportedComparator
Verbatim
e0“CsCBF expression was differentially induced by cold, heat, PEG, salinity, ABA, GA, MeJA and SA stresses.”
t0“tea plant (Camellia sinensis)”
Claim · model-written summary
Cold significantly induced CsCBF genes.
Not reportedComparator
Verbatim
e0“the CsCBF genes were significantly induced by cold treatments”
t0“tea plant (Camellia sinensis)”
Claim · model-written summary
CsCBF3 overexpression increased cold tolerance in Arabidopsis compared with wild type.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- overexpressed the CsCBF3 genee0
- Gene
- CsCBF3e0
- Outcome
- cold tolerancee0
- Direction
- increasede0
- Comparator
- wild-type Arabidopsis plante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we overexpressed the CsCBF3 gene in Arabidopsis thaliana plants. The resulting transgenic plants showed increased cold tolerance compared with the wild-type Arabidopsis plant.”
Claim · model-written summary
Enhanced cold tolerance may act through an ABA-independent pathway.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- enhanced cold tolerancee0
- Qualifier
- ABA-independent pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis plant. The enhanced cold tolerance of the transgenic plants was potentially achieved through an ABA-independent pathway.”
The same questions in other papers
(-)-methyl jasmonate · molecular: expression · Camellia sinensis (black tea)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
1 paper more in this group
See all 5 papers →(+)-abscisic acid · molecular: expression · Camellia sinensis (black tea)Positive and negative results
3 papers in the same direction as this paper · 3 in the opposite direction
- negative
- no effect
- negative
3 papers more in this group
See all 7 papers →salicylic acid · molecular: expression · Camellia sinensis (black tea)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
drought · molecular: expression · Camellia sinensis (black tea)Results agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →cold · molecular: expression · Camellia sinensis (black tea)Results agree
9 papers in the same direction as this paper
- positive
- positive
- positive
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.