Abstract
In vitro androgenesis is a unique model for producing homozygous doubled haploid plants. The use of haploid biotechnology accelerates to obtain of doubled haploid plants, which is very important in rice breeding. The purpose of this work is to improve the production of doubled haploids in rice anther culture in vitro and selection of doubled haploid plants with valuable traits. The study the influence of nutrient media on the production of calli and plant regeneration processes in anther culture of 35 rice genotypes was revealed a significant influence of nutrient media on callus production. It was shown that the addition to culture medium phytohormones ratio with high level of cytokinin (5.0 mg/L BAP) and a low level of auxin (0.5 mg/L NAA), supplemented with amino acid composition promotes high production of green regenerated plants (68.75%) compared to albino plants (31.25%). As a result, doubled haploid lines of the glutinous variety Violetta were selected, which characterized by a low amylose content variation (from 1.86 to 2.80%). These doubled haploids are superior to the original variety in some yield traits and represent valuable breeding material.
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Claims 4
Claim · model-written summary
A medium with high cytokinin (5.0 mg/L BAP), low auxin (0.5 mg/L NAA) and amino acids promoted high production of green regenerated plants (68.75%) compared with albino plants (31.25%).
- Taxon
- rice
Oryza sativat0 - Treatment
- high level of cytokinin (5.0 mg/L BAP) and a low level of auxin (0.5 mg/L NAA), supplemented with amino acid compositione1
- Outcome
- production of green regenerated plantse1
- Direction
- promotes high productione1
- Value
- 68.75%e1
- Qualifier
- 31.25%e1
- Comparator
- albino plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Creation of rice doubled haploids”
e1“the addition to culture medium phytohormones ratio with high level of cytokinin (5.0 mg/L BAP) and a low level of auxin (0.5 mg/L NAA), supplemented with amino acid composition promotes high production of green regenerated plants (68.75%) compared to albino plants (31.25%)”
Claim · model-written summary
Nutrient media had a significant influence on callus production in anther culture of 35 rice genotypes.
- Taxon
- riceOryza sativat0
- Material
- 35 rice genotypese2
- Treatment
- nutrient mediae2
- Outcome
- callus productione2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Creation of rice doubled haploids”
e2“in anther culture of 35 rice genotypes was revealed a significant influence of nutrient media on callus production”
Claim · model-written summary
Doubled haploid lines of the glutinous variety Violetta had amylose content variation from 1.86 to 2.80%.
- Taxon
- riceOryza sativat0
- Material
- glutinous variety Violettae3
- Outcome
- amylose content variatione3
- Value
- 1.86 to 2.80%e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Creation of rice doubled haploids”
e3“doubled haploid lines of the glutinous variety Violetta were selected, which characterized by a low amylose content variation (from 1.86 to 2.80%)”
Claim · model-written summary
The doubled haploids are superior to the original variety in some yield traits.
- Taxon
- riceOryza sativat0
- Material
- doubled haploidse4
- Outcome
- yield traitse4
- Direction
- superiore4
- Comparator
- the original varietye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Creation of rice doubled haploids”
e4“These doubled haploids are superior to the original variety in some yield traits”
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