Abstract
Tiller number and grain size are important agronomic traits that determine grain yield in rice. Here, we demonstrate that DEFECTIVE TILLER GROWTH 1 (DTG1), a member of the casein kinase 1 protein family, exerts a co-regulatory effect on tiller number and grain size. We identified a single amino acid substitution in DTG1 (I357K) that caused a decrease in tiller number and an increase in grain size in NIL- dtg1 . Genetic analyses revealed that DTG1 plays a pivotal role in regulation of tillering and grain size. The DTG1 I357K allelic variant exhibited robust functionality in suppressing tillering. We show that DTG1 is preferentially expressed in tiller buds and young panicles, and negatively regulates grain size by restricting cell proliferation in spikelet hulls. We further confirm that DTG1 functioned in grain size regulation by directly interacting with Grain Width 2 (GW2), a critical grain size regulator in rice. The CRISPR/Cas9-mediated elimination of DTG1 significantly enhanced tiller number and grain size, thereby increasing rice grain yield under field conditions, thus highlighting potential value of DTG1 in rice breeding.
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Claims 7
Claim · model-written summary
The DTG1 I357K substitution decreased tiller number in NIL-dtg1 rice.
- Taxon
- rice
Oryza sativat1 - Material
- NIL- dtg1e0
- Treatment
- single amino acid substitution in DTG1 (I357K)e0
- Gene
- DTG1e0
- Outcome
- tiller numbere0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“grain size in rice”
e0“We identified a single amino acid substitution in DTG1 (I357K) that caused a decrease in tiller number and an increase in grain size in NIL- dtg1”
Claim · model-written summary
The DTG1 I357K substitution increased grain size in NIL-dtg1 rice.
- Taxon
- riceOryza sativat1
- Material
- NIL- dtg1e0
- Treatment
- single amino acid substitution in DTG1 (I357K)e0
- Gene
- DTG1e0
- Outcome
- grain sizee0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“grain size in rice”
e0“We identified a single amino acid substitution in DTG1 (I357K) that caused a decrease in tiller number and an increase in grain size in NIL- dtg1”
Claim · model-written summary
DTG1 plays a pivotal role in regulating tillering and grain size in rice.
- Taxon
- riceOryza sativat1
- Gene
- DTG1e4
- Outcome
- regulation of tillering and grain sizee4
- Qualifier
- pivotal rolee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“grain size in rice”
e4“Genetic analyses revealed that DTG1 plays a pivotal role in regulation of tillering and grain size”
Claim · model-written summary
DTG1 is preferentially expressed in tiller buds and young panicles of rice.
- Taxon
- riceOryza sativat1
- Organ / stage
- tiller buds and young paniclese1
- Gene
- DTG1e1
- Outcome
- DTG1 is preferentially expressede1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“grain size in rice”
e1“DTG1 is preferentially expressed in tiller buds and young panicles”
Claim · model-written summary
DTG1 regulates grain size by directly interacting with Grain Width 2 (GW2) in rice.
- Taxon
- riceOryza sativat1
- Gene
- Grain Width 2 (GW2)e3
- Outcome
- grain size regulatione3
- Qualifier
- directly interactinge3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“grain size in rice”
e3“DTG1 functioned in grain size regulation by directly interacting with Grain Width 2 (GW2)”
Claim · model-written summary
CRISPR/Cas9-mediated elimination of DTG1 significantly enhanced tiller number and grain size in rice under field conditions.
- Taxon
- riceOryza sativat1
- Treatment
- CRISPR/Cas9-mediated elimination of DTG1e5
- Gene
- DTG1e5
- Outcome
- tiller number and grain sizee5
- Direction
- enhancede5
- Setting
- field conditionse5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“grain size in rice”
e5“The CRISPR/Cas9-mediated elimination of DTG1 significantly enhanced tiller number and grain size, thereby increasing rice grain yield under field conditions”
Claim · model-written summary
CRISPR/Cas9-mediated elimination of DTG1 increased rice grain yield under field conditions.
- Taxon
- riceOryza sativat1
- Treatment
- CRISPR/Cas9-mediated elimination of DTG1e5
- Outcome
- rice grain yielde5
- Direction
- increasinge5
- Setting
- field conditionse5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“grain size in rice”
e5“The CRISPR/Cas9-mediated elimination of DTG1 significantly enhanced tiller number and grain size, thereby increasing rice grain yield under field conditions”
The same questions in other papers
amino acid · yield · Oryza sativa (rice)Results agree
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586 papers in the same direction as this paper · 115 in the opposite direction · 27 reporting no difference from an alternative
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See all 729 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.