Abstract
The current simple and crude facilities make melon production more susceptible to cold stress during off-season cultivation in China. The ABA signalling pathway is an important target for breeding cold-tolerant melon. Cold-tolerant No. 330 and cold-sensitive No. 410 oriental melon genotypes were used to analyse the relationship between ABA and cold tolerance. 12 CmPYLs, ABA receptors, were identified from the melon genome database according to sequence alignment and phylogenetic analysis. Gene function of CmPYL6 in cold tolerance was analysed using VIGS in No. 330 and overexpression in Arabidopsis WT. A total of 12 CmPYL members contain the representative domain and conserved sites. Under cold treatment, No.330 seedlings had lower electrolyte leakage and MDA content, higher ABA content and CmPYL6 expression than seedlings of No. 410. Exogenous application of ABA upregulated expression of CmPYL6 and enhanced cold tolerance of both genotypes, while inhibiting ABA accumulation reduced expression of CmPYL6 and cold tolerance of both genotypes. CmPYL6-silenced No. 330 seedlings had reduced cold tolerance, increased electrolyte leakage and MDA content as well as limited proline and soluble sugar content, while CmPYL6 overexpressed transgenic Arabidopsis plants had enhanced cold tolerance, with limited electrolyte leakage and MDA content, as well as increased proline and soluble sugar content. The CmPYL6 gene is probably an important ABA receptor in regulating cold tolerance of oriental melon. Our study provides a direction for improving breeding of cold tolerance of oriental melon.
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Claims 14
Claim · model-written summary
No.330 seedlings had lower electrolyte leakage and MDA content than No. 410 seedlings under cold treatment.
- Taxon
- oriental melon
.jpg?width=60)
Cucumis melot0 - Material
- No.330e1
- Organ / stage
- seedlingse1
- Treatment
- cold treatmente2
- Outcome
- electrolyte leakage and MDA contente1
- Direction
- lowere1
- Comparator
- seedlings of No. 410e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“oriental melon”
e1“No.330 seedlings had lower electrolyte leakage and MDA content, higher ABA content and CmPYL6 expression than seedlings of No. 410”
e2“Under cold treatment”
Claim · model-written summary
No.330 seedlings had higher ABA content and CmPYL6 expression than No. 410 seedlings under cold treatment.
- Taxon
- oriental melonCucumis melot0
- Material
- No.330e1
- Treatment
- cold treatmente2
- Gene
- CmPYL6e1
- Outcome
- ABA content and CmPYL6 expressione1
- Direction
- highere1
- Comparator
- seedlings of No. 410e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“oriental melon”
e1“No.330 seedlings had lower electrolyte leakage and MDA content, higher ABA content and CmPYL6 expression than seedlings of No. 410”
e2“Under cold treatment”
Claim · model-written summary
Exogenous application of ABA upregulated CmPYL6 expression in oriental melon.
- Taxon
- oriental melonCucumis melot0
- Treatment
- Exogenous application of ABAe3
- Gene
- CmPYL6e3
- Outcome
- expression of CmPYL6e3
- Direction
- upregulatede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“oriental melon”
e3“Exogenous application of ABA upregulated expression of CmPYL6 and enhanced cold tolerance of both genotypes”
Claim · model-written summary
Exogenous application of ABA enhanced cold tolerance of both genotypes.
- Taxon
- oriental melonCucumis melot0
- Treatment
- Exogenous application of ABAe3
- Outcome
- cold tolerancee3
- Direction
- enhancede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“oriental melon”
e3“Exogenous application of ABA upregulated expression of CmPYL6 and enhanced cold tolerance of both genotypes”
Claim · model-written summary
Inhibiting ABA accumulation reduced CmPYL6 expression in oriental melon.
- Taxon
- oriental melonCucumis melot0
- Treatment
- inhibiting ABA accumulatione4
- Gene
- CmPYL6e4
- Outcome
- expression of CmPYL6e4
- Direction
- reducede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“oriental melon”
e4“inhibiting ABA accumulation reduced expression of CmPYL6 and cold tolerance of both genotypes”
Claim · model-written summary
Inhibiting ABA accumulation reduced cold tolerance of both genotypes.
- Taxon
- oriental melonCucumis melot0
- Treatment
- inhibiting ABA accumulatione4
- Outcome
- cold tolerancee4
- Direction
- reducede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“oriental melon”
e4“inhibiting ABA accumulation reduced expression of CmPYL6 and cold tolerance of both genotypes”
Claim · model-written summary
CmPYL6-silenced No. 330 seedlings had reduced cold tolerance.
- Taxon
- oriental melonCucumis melot0
- Material
- No. 330e5
- Treatment
- CmPYL6-silencede5
- Gene
- CmPYL6e5
- Outcome
- cold tolerancee5
- Direction
- reducede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“oriental melon”
e5“CmPYL6-silenced No. 330 seedlings had reduced cold tolerance, increased electrolyte leakage and MDA content”
Claim · model-written summary
CmPYL6-silenced No. 330 seedlings had increased electrolyte leakage and MDA content.
- Taxon
- oriental melonCucumis melot0
- Treatment
- CmPYL6-silencede5
- Outcome
- electrolyte leakage and MDA contente5
- Direction
- increasede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“oriental melon”
e5“CmPYL6-silenced No. 330 seedlings had reduced cold tolerance, increased electrolyte leakage and MDA content”
Claim · model-written summary
CmPYL6-silenced No. 330 seedlings had limited proline and soluble sugar content.
- Taxon
- oriental melonCucumis melot0
- Treatment
- CmPYL6-silencede5
- Outcome
- proline and soluble sugar contente6
- Direction
- limitede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“oriental melon”
e5“CmPYL6-silenced No. 330 seedlings had reduced cold tolerance, increased electrolyte leakage and MDA content”
e6“limited proline and soluble sugar content”
Claim · model-written summary
CmPYL6 overexpression in Arabidopsis enhanced cold tolerance relative to wild type.
- Taxon
- Arabidopsis
Arabidopsise7 - Treatment
- CmPYL6 overexpressede7
- Gene
- CmPYL6e7
- Outcome
- cold tolerancee7
- Direction
- enhancede7
- Comparator
- Arabidopsis WTe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“CmPYL6 overexpressed transgenic Arabidopsis plants had enhanced cold tolerance, with limited electrolyte leakage and MDA content, as well as increased proline and soluble sugar content”
e2“overexpression in Arabidopsis WT”
Claim · model-written summary
CmPYL6 overexpressed Arabidopsis had limited electrolyte leakage and MDA content.
- Taxon
- ArabidopsisArabidopsise7
- Treatment
- CmPYL6 overexpressede7
- Outcome
- electrolyte leakage and MDA contente7
- Direction
- limitede7
- Comparator
- Arabidopsis WTe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“CmPYL6 overexpressed transgenic Arabidopsis plants had enhanced cold tolerance, with limited electrolyte leakage and MDA content, as well as increased proline and soluble sugar content”
e2“overexpression in Arabidopsis WT”
Claim · model-written summary
CmPYL6 overexpressed Arabidopsis had increased proline and soluble sugar content.
- Taxon
- ArabidopsisArabidopsise7
- Treatment
- CmPYL6 overexpressede7
- Outcome
- proline and soluble sugar contente7
- Direction
- increasede7
- Comparator
- Arabidopsis WTe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“CmPYL6 overexpressed transgenic Arabidopsis plants had enhanced cold tolerance, with limited electrolyte leakage and MDA content, as well as increased proline and soluble sugar content”
e2“overexpression in Arabidopsis WT”
Claim · model-written summary
Twelve CmPYL members were identified in the melon genome database.
- Taxon
- melonCucumis meloe0
- Outcome
- CmPYLse0
- Value
- 12e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“12 CmPYLs, ABA receptors, were identified from the melon genome database”
Claim · model-written summary
CmPYL6 is probably an important ABA receptor in regulating cold tolerance of oriental melon.
- Taxon
- oriental melonCucumis meloe0
- Gene
- CmPYL6e0
- Outcome
- cold tolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The CmPYL6 gene is probably an important ABA receptor in regulating cold tolerance of oriental melon”
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See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.