Abstract
Plant spotted leaf ( spl ) mutants are useful to reveal the regulatory mechanisms of immune responses. Thus, in crop plants, their agronomic traits, especially the grain quality are usually ignored. Here, we characterized a rice spl mutant named spl-A ( spotted leaf mutant from A814 ) that shows autoimmunity, broad-spectrum disease resistance and growth deterioration including decreased rice quality. A single nucleotide mutation of C1144T, which leads to change of the 382 nd proline to serine, in the gene encoding the ATPases associated with diverse cellular activities (AAA)-type ATPase LRD6-6 is responsible for the phenotype of the spl-A mutant. Mechanistically, this mutation impairs LRD6-6 ATPase activity and disrupts its interaction with endosomal sorting complex required for transport (ESCRT)-III subunits OsSNF7.1/7.2/7.3. And thus, leading to compromise of multivesicular bodies (MVBs)-mediated vesicle trafficking and accumulation of ubiquitinated proteins in both leaves and seeds of spl-A . Therefore, the immune response of spl-A is activated, and the growth and grain quality are deteriorated. Our study identifies a new amino acid residue that important for LRD6-6 and provides new insight into our understanding of how MVBs-mediated vesicle trafficking regulates plant immunity and growth, including grain quality in rice.
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Claims 8
Claim · model-written summary
The spl-A rice mutant shows autoimmunity, broad-spectrum disease resistance and growth deterioration.
- Taxon
- rice
Oryza sativae1 - Material
- spl-Ae1
- Outcome
- autoimmunity, broad-spectrum disease resistance and growth deterioratione2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a rice spl mutant named spl-A ( spotted leaf mutant from A814 )”
e2“shows autoimmunity, broad-spectrum disease resistance and growth deterioration”
Claim · model-written summary
The spl-A rice mutant shows decreased rice quality.
- Taxon
- riceOryza sativae1
- Material
- spl-Ae1
- Outcome
- rice qualitye3
- Direction
- decreasede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a rice spl mutant named spl-A ( spotted leaf mutant from A814 )”
e3“growth deterioration including decreased rice quality”
Claim · model-written summary
The C1144T mutation in LRD6-6 changes the 382nd proline to serine.
- Taxon
- riceOryza sativae1
- Gene
- LRD6-6e4
- Outcome
- change of the 382 nd proline to serinee4
- Qualifier
- C1144Te4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a rice spl mutant named spl-A ( spotted leaf mutant from A814 )”
e4“A single nucleotide mutation of C1144T, which leads to change of the 382 nd proline to serine, in the gene encoding the ATPases associated with diverse cellular activities (AAA)-type ATPase LRD6-6”
Claim · model-written summary
The C1144T mutation in LRD6-6 is responsible for the phenotype of the spl-A mutant.
- Taxon
- riceOryza sativae1
- Gene
- LRD6-6e4
- Outcome
- phenotype of the spl-A mutante5
- Qualifier
- C1144Te4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a rice spl mutant named spl-A ( spotted leaf mutant from A814 )”
e4“A single nucleotide mutation of C1144T, which leads to change of the 382 nd proline to serine, in the gene encoding the ATPases associated with diverse cellular activities (AAA)-type ATPase LRD6-6”
e5“is responsible for the phenotype of the spl-A mutant”
Claim · model-written summary
The mutation impairs LRD6-6 ATPase activity.
- Taxon
- riceOryza sativae1
- Treatment
- this mutatione6
- Gene
- LRD6-6e6
- Outcome
- ATPase activitye6
- Direction
- impairse6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a rice spl mutant named spl-A ( spotted leaf mutant from A814 )”
e6“this mutation impairs LRD6-6 ATPase activity”
Claim · model-written summary
The mutation disrupts the interaction of LRD6-6 with ESCRT-III subunits OsSNF7.1/7.2/7.3.
- Taxon
- riceOryza sativae1
- Treatment
- this mutatione6
- Gene
- OsSNF7.1/7.2/7.3e7
- Outcome
- interactione7
- Direction
- disruptse7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a rice spl mutant named spl-A ( spotted leaf mutant from A814 )”
e6“this mutation impairs LRD6-6 ATPase activity”
e7“disrupts its interaction with endosomal sorting complex required for transport (ESCRT)-III subunits OsSNF7.1/7.2/7.3”
Claim · model-written summary
The mutation compromises MVB-mediated vesicle trafficking in rice.
- Taxon
- riceOryza sativae1
- Treatment
- this mutatione6
- Outcome
- multivesicular bodies (MVBs)-mediated vesicle traffickinge3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a rice spl mutant named spl-A ( spotted leaf mutant from A814 )”
e6“this mutation impairs LRD6-6 ATPase activity”
e3“leading to compromise of multivesicular bodies (MVBs)-mediated vesicle trafficking”
Claim · model-written summary
Ubiquitinated proteins accumulate in both leaves and seeds of spl-A.
- Taxon
- riceOryza sativae1
- Material
- spl-Ae7
- Organ / stage
- both leaves and seedse7
- Outcome
- accumulation of ubiquitinated proteinse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a rice spl mutant named spl-A ( spotted leaf mutant from A814 )”
e7“accumulation of ubiquitinated proteins in both leaves and seeds of spl-A”
The same questions in other papers
loss of function · physiology: enzyme activity · Oryza sativa (rice)Positive and negative results
5 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
5 papers more in this group
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