Abstract
Leaf senescence is a developmental program regulated by both endogenous and environmental cues. Abiotic stresses such as nutrient deprivation can induce premature leaf senescence, which profoundly impacts plant growth and crop yield. However, the molecular mechanisms underlying stress-induced senescence are not fully understood. In this work, employing a carbon deprivation (C-deprivation)-induced senescence assay in Arabidopsis seedlings, we identified PLEIOTROPIC REGULATORY LOCUS 1 (PRL1), a component of the NineTeen Complex, as a negative regulator of C-deprivation-induced senescence. Furthermore, we demonstrated that PRL1 directly interacts with the RPA2A subunit of the single-stranded DNA-binding Replication Protein A (RPA) complex. Consistently, the loss of RPA2A leads to premature senescence, while increased expression of RPA2A inhibits senescence. Moreover, overexpression of RPA2A reverses the accelerated senescence in prl1 mutants, and the interaction with PRL1 stabilizes RPA2A under C-deprivation. In summary, our findings reveal the involvement of the PRL1-RPA2A functional module in C-deprivation-induced plant senescence.
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Claims 6
Claim · model-written summary
PRL1 negatively regulates carbon-deprivation-induced senescence.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- PLEIOTROPIC REGULATORY LOCUS 1 (PRL1)e0
- Outcome
- C-deprivation-induced senescencee0
- Direction
- negative regulatore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified PLEIOTROPIC REGULATORY LOCUS 1 (PRL1), a component of the NineTeen Complex, as a negative regulator of C-deprivation-induced senescence”
t0“Arabidopsis”
Claim · model-written summary
PRL1 directly interacts with RPA2A.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- PRL1e0
- Outcome
- directly interacts with the RPA2A subunite0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PRL1 directly interacts with the RPA2A subunit of the single-stranded DNA-binding Replication Protein A (RPA) complex”
t0“Arabidopsis”
Claim · model-written summary
Loss of RPA2A causes premature senescence.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- loss of RPA2Ae0
- Gene
- RPA2Ae0
- Outcome
- premature senescencee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the loss of RPA2A leads to premature senescence”
t0“Arabidopsis”
Claim · model-written summary
Increased RPA2A expression inhibits senescence.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- increased expression of RPA2Ae0
- Gene
- RPA2Ae0
- Outcome
- senescencee0
- Direction
- inhibitse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased expression of RPA2A inhibits senescence”
t0“Arabidopsis”
Claim · model-written summary
RPA2A overexpression reverses accelerated senescence in prl1 mutants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- prl1 mutantse0
- Treatment
- overexpression of RPA2Ae0
- Gene
- RPA2Ae0
- Outcome
- accelerated senescencee0
- Direction
- reversese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of RPA2A reverses the accelerated senescence in prl1 mutants”
t0“Arabidopsis”
Claim · model-written summary
PRL1 interaction stabilizes RPA2A under carbon deprivation.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- PRL1e0
- Outcome
- stabilizes RPA2Ae0
- Qualifier
- under C-deprivatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the interaction with PRL1 stabilizes RPA2A under C-deprivation”
t0“Arabidopsis”
The same questions in other papers
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- negative
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