Abstract
Abstract Here, we elucidate the interaction between IAA and melatonin (MT) in response to chilling in cucumber. The results showed that chilling stress induced the increase of endogenous MT and IAA, and the application of MT promoted the synthesis of IAA, while IAA could not affect endogenous MT content under chilling stress. Moreover, MT and IAA application both remarkably increased the chilling tolerance of cucumber seedlings in terms of lower contents of MDA and ROS, higher mRNA abundance of cold response genes, net photosynthetic rate ( P n ), maximum regeneration rate of ribulose‐1,5‐diphosphate (J max ), Rubisco maximum carboxylation efficiency (V cmax ), the activities and gene expression of RCA and Rubisco, as well as the content of active P700 (I/I 0 ) and photosynthetic electron transport, compared with the plants in H 2 O treatment. Further analysis revealed that the inhibition of IAA transportation significantly reduced the chilling tolerance induced by MT, whereas the inhibition of endogenous MT did not affect the chilling tolerance induced by IAA. Meanwhile, we found that overexpression of the MT biosynthesis gene CsASMT increased the chilling tolerance, which was blocked by inhibition of endogenous IAA, and the silence of IAA biosynthesis gene CsYUCCA10 decreased the chilling tolerance of cucumber, which could not be alleviated by MT. These data implied IAA acted as a downstream signal to participate in the MT‐induced chilling tolerance of cucumber seedlings. The study has implications for the production of greenhouse cucumber in winter seasons.
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Claims 18
Claim · model-written summary
Chilling stress increased endogenous MT in cucumber.
- Taxon
- cucumber
Cucumis sativust0 - Treatment
- chilling stresse0
- Outcome
- endogenous MTe0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“chilling stress induced the increase of endogenous MT and IAA”
t0“cucumber”
Claim · model-written summary
Chilling stress increased endogenous IAA in cucumber.
- Taxon
- cucumberCucumis sativust0
- Treatment
- chilling stresse0
- Outcome
- IAAe0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“chilling stress induced the increase of endogenous MT and IAA”
t0“cucumber”
Claim · model-written summary
MT application promoted IAA synthesis in cucumber.
- Taxon
- cucumberCucumis sativust0
- Treatment
- application of MTe0
- Outcome
- synthesis of IAAe0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the application of MT promoted the synthesis of IAA”
t0“cucumber”
Claim · model-written summary
IAA did not affect endogenous MT content under chilling stress.
- Taxon
- cucumberCucumis sativust0
- Treatment
- IAAe0
- Outcome
- endogenous MT contente0
- Direction
- could not affecte0
- Qualifier
- under chilling stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IAA could not affect endogenous MT content under chilling stress”
t0“cucumber”
Claim · model-written summary
MT application increased chilling tolerance of cucumber seedlings versus H 2 O treatment.
- Taxon
- cucumber seedlingsCucumis sativuse0
- Treatment
- MTe0
- Outcome
- chilling tolerancee0
- Direction
- increasede0
- Comparator
- H 2 O treatmente1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MT and IAA application both remarkably increased the chilling tolerance of cucumber seedlings”
e1“compared with the plants in H 2 O treatment”
Claim · model-written summary
IAA application increased chilling tolerance of cucumber seedlings versus H 2 O treatment.
- Taxon
- cucumber seedlingsCucumis sativuse0
- Treatment
- IAAe0
- Outcome
- chilling tolerancee0
- Direction
- increasede0
- Comparator
- H 2 O treatmente1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MT and IAA application both remarkably increased the chilling tolerance of cucumber seedlings”
e1“compared with the plants in H 2 O treatment”
Claim · model-written summary
MT and IAA application lowered MDA and ROS in cucumber seedlings.
- Taxon
- cucumber seedlingsCucumis sativuse0
- Treatment
- MT and IAA applicatione0
- Outcome
- contents of MDA and ROSe0
- Direction
- lowere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MT and IAA application both remarkably increased the chilling tolerance of cucumber seedlings in terms of lower contents of MDA and ROS”
Claim · model-written summary
MT and IAA application increased cold response gene mRNA abundance in cucumber seedlings.
- Taxon
- cucumber seedlingsCucumis sativuse1
- Outcome
- mRNA abundance of cold response genese0
- Direction
- highere0
Not reportedTreatment
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher mRNA abundance of cold response genes”
e1“chilling tolerance of cucumber seedlings in terms of lower contents of MDA and ROS”
Claim · model-written summary
MT and IAA application increased photosynthetic capacity measures in cucumber seedlings.
- Taxon
- cucumber seedlingsCucumis sativuse1
- Outcome
- net photosynthetic rate ( P n ), maximum regeneration rate of ribulose‐1,5‐diphosphate (J max ), Rubisco maximum carboxylation efficiency (V cmax )e0
- Direction
- highere2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“net photosynthetic rate ( P n ), maximum regeneration rate of ribulose‐1,5‐diphosphate (J max ), Rubisco maximum carboxylation efficiency (V cmax )”
e1“chilling tolerance of cucumber seedlings in terms of lower contents of MDA and ROS”
e2“higher mRNA abundance”
Claim · model-written summary
MT and IAA application increased RCA and Rubisco activity and expression.
- Taxon
- cucumber seedlingsCucumis sativuse2
- Outcome
- activities and gene expression of RCA and Rubiscoe0
- Direction
- highere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the activities and gene expression of RCA and Rubisco”
e1“higher mRNA abundance”
e2“chilling tolerance of cucumber seedlings in terms of lower contents of MDA and ROS”
Claim · model-written summary
MT and IAA application increased active P700 content and photosynthetic electron transport.
- Taxon
- cucumber seedlingsCucumis sativuse2
- Outcome
- content of active P700 (I/I 0 ) and photosynthetic electron transporte0
- Direction
- highere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the content of active P700 (I/I 0 ) and photosynthetic electron transport”
e1“higher mRNA abundance”
e2“chilling tolerance of cucumber seedlings in terms of lower contents of MDA and ROS”
Claim · model-written summary
Inhibiting IAA transportation reduced MT-induced chilling tolerance.
- Taxon
- cucumberCucumis sativust0
- Treatment
- inhibition of IAA transportatione0
- Outcome
- chilling tolerance induced by MTe0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inhibition of IAA transportation significantly reduced the chilling tolerance induced by MT”
t0“cucumber”
Claim · model-written summary
Inhibiting endogenous MT did not affect IAA-induced chilling tolerance.
- Taxon
- cucumberCucumis sativust0
- Treatment
- inhibition of endogenous MTe0
- Outcome
- chilling tolerance induced by IAAe0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inhibition of endogenous MT did not affect the chilling tolerance induced by IAA”
t0“cucumber”
Claim · model-written summary
Overexpression of CsASMT increased cucumber chilling tolerance.
- Taxon
- cucumberCucumis sativust0
- Treatment
- overexpression of the MT biosynthesis gene CsASMTe0
- Gene
- CsASMTe0
- Outcome
- chilling tolerancee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of the MT biosynthesis gene CsASMT increased the chilling tolerance”
t0“cucumber”
Claim · model-written summary
Inhibition of endogenous IAA blocked the tolerance increase from CsASMT overexpression.
- Taxon
- cucumberCucumis sativust0
- Treatment
- inhibition of endogenous IAAe0
- Gene
- CsASMTe0
- Outcome
- chilling tolerancee0
- Direction
- blockede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of the MT biosynthesis gene CsASMT increased the chilling tolerance, which was blocked by inhibition of endogenous IAA”
t0“cucumber”
Claim · model-written summary
Silencing CsYUCCA10 decreased cucumber chilling tolerance.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- silence of IAA biosynthesis gene CsYUCCA10e0
- Gene
- CsYUCCA10e0
- Outcome
- chilling tolerancee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silence of IAA biosynthesis gene CsYUCCA10 decreased the chilling tolerance of cucumber”
Claim · model-written summary
MT did not alleviate the chilling tolerance decrease following CsYUCCA10 silencing.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- MTe0
- Gene
- CsYUCCA10e0
- Outcome
- chilling tolerancee0
- Direction
- could not be alleviatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silence of IAA biosynthesis gene CsYUCCA10 decreased the chilling tolerance of cucumber, which could not be alleviated by MT”
Claim · model-written summary
IAA was inferred to act downstream of MT in cucumber chilling tolerance.
- Taxon
- cucumber seedlingsCucumis sativuse0
- Compound
- IAAe0
- Outcome
- MT‐induced chilling tolerancee0
- Qualifier
- downstream signale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IAA acted as a downstream signal to participate in the MT‐induced chilling tolerance of cucumber seedlings”
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