Abstract
Colchicine is an alkaloid that features a carbotricyclic structure, consisting of a 5,6,7,9-tetrahydrobenzo[a]heptalene core with four methoxy groups at positions 1, 2, 3, and 10, an oxo group at position 9, and an acetamido group at position 7. It is derived from plants of the Colchicum genus. Colchicine acts as a microtubule-destabilizing agent and is a plant metabolite. This study investigates the effects of colchicine on the mitotic cycle in root apical meristems of Allium cepa var aggregatum (onion). Onion bulbs were treated with different concentrations of colchicine (0.5%, 1.0%, and 1.5%) to analyze its impact on mitosis inhibition and chromosomal aberrations. The colchicine-treated root meristem cells showed a range of mitotic abnormalities, including laggards, stickiness, vagrant chromosomes, binucleated cells, and nuclear lesions. The study also observed a dose-dependent increase in aberrations, with higher colchicine concentrations leading to more pronounced effects. Chromosome preparations were analyzed under a light microscope, revealing disruptions in metaphase alignment and anaphase movement. Mitotic Index and frequency of abberant cells was also calculated. The results highlight colchicine’s ability to inhibit normal cell division and form dumbbell shaped nucleus, which was observed for the first time, providing insight into its potential applications in cytogenetic studies.
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Claims 5
Claim · model-written summary
Colchicine-treated root meristem cells showed mitotic abnormalities including laggards, stickiness, vagrant chromosomes, binucleated cells and nuclear lesions.
- Taxon
- Allium cepa var aggregatum
Allium cepae1 - Organ / stage
- root meristem cellse0
- Treatment
- colchicinee0
- Outcome
- mitotic abnormalitiese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The colchicine-treated root meristem cells showed a range of mitotic abnormalities, including laggards, stickiness, vagrant chromosomes, binucleated cells, and nuclear lesions”
e1“in root apical meristems of Allium cepa var aggregatum (onion)”
Claim · model-written summary
Higher colchicine concentrations produced a dose-dependent increase in aberrations.
- Taxon
- Allium cepa var aggregatumAllium cepae1
- Treatment
- higher colchicine concentrationse0
- Outcome
- aberrationse0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The study also observed a dose-dependent increase in aberrations, with higher colchicine concentrations leading to more pronounced effects”
e1“in root apical meristems of Allium cepa var aggregatum (onion)”
Claim · model-written summary
Colchicine disrupted metaphase alignment and anaphase movement in chromosome preparations.
- Taxon
- Allium cepa var aggregatumAllium cepae1
- Organ / stage
- root apical meristemse1
- Outcome
- metaphase alignment and anaphase movemente0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chromosome preparations were analyzed under a light microscope, revealing disruptions in metaphase alignment and anaphase movement”
e1“in root apical meristems of Allium cepa var aggregatum (onion)”
Claim · model-written summary
Colchicine inhibits normal cell division in onion root meristems.
- Taxon
- Allium cepa var aggregatumAllium cepae1
- Treatment
- colchicinee2
- Outcome
- normal cell divisione0
- Direction
- inhibite0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“to inhibit normal cell division”
e1“in root apical meristems of Allium cepa var aggregatum (onion)”
e2“The results highlight colchicine”
Claim · model-written summary
Colchicine formed dumbbell-shaped nuclei, observed for the first time.
- Taxon
- Allium cepa var aggregatumAllium cepae1
- Outcome
- dumbbell shaped nucleuse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“form dumbbell shaped nucleus, which was observed for the first time”
e1“in root apical meristems of Allium cepa var aggregatum (onion)”
The same questions in other papers
colchicine · cytology · Allium cepa (onion)Results differ
3 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- negative
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See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.