Abstract
Abstract The pathogen Colletotrichum gloeosporioides causes anthracnose, a serious threat to tea trees around the world, particularly in warm and humid regions. RNA-Seq data have previously indicated NAC transcription factors are involved in anthracnose resistance, but underlying mechanisms remain unclear. The BiFC, Split-LUC, and Co-IP assays validated the interaction between CsbHLH62 and CsNAC17 identified through yeast two-hybrid (Y2H) screening. CsNAC17 or CsbHLH62 overexpression enhanced anthracnose resistance, as well as enhanced levels of H2O2, hypersensitivity, and cell death in Nicotiana benthamiana. The NBS-LRR gene CsRPM1 is regulated by CsNAC17 by binding directly to its promoter (i.e. CACG, CATGTG), while CsbHLH62 facilitates CsNAC17’s binding and increases transcriptional activity of CsRPM1. Additionally, transient silencing of CsNAC17 and CsbHLH62 in tea plant leaves using the virus-induced gene silencing (VIGS) system resulted in decreased resistance to anthracnose. Conversely, transient overexpression of CsNAC17 and CsbHLH62 in tea leaves significantly enhanced the resistance against anthracnose. Based on these results, it appears that CsbHLH62 facilitates the activity of CsNAC17 on CsRPM1, contributing to increased anthracnose resistance.
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Claims 7
Claim · model-written summary
CsbHLH62 and CsNAC17 interact, as validated by BiFC, Split-LUC and Co-IP assays.
- Taxon
- Camellia sinensis
Camellia sinensist0 - Gene
- CsbHLH62 and CsNAC17e1
- Outcome
- interaction between CsbHLH62 and CsNAC17e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“CsNAC17 enhances resistance to Colletotrichum gloeosporioides by interacting with CsbHLH62 in Camellia sinensis”
e1“The BiFC, Split-LUC, and Co-IP assays validated the interaction between CsbHLH62 and CsNAC17”
Claim · model-written summary
Overexpression of CsNAC17 or CsbHLH62 enhanced anthracnose resistance.
- Taxon
- Camellia sinensisCamellia sinensist0
- Treatment
- CsNAC17 or CsbHLH62 overexpressione2
- Interactor
- Colletotrichum gloeosporioides
Colletotrichum gloeosporioidese0pathogen - Gene
- CsNAC17 or CsbHLH62e2
- Outcome
- anthracnose resistancee2
- Direction
- enhancede2
- Qualifier
- in Nicotiana benthamianae2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“CsNAC17 enhances resistance to Colletotrichum gloeosporioides by interacting with CsbHLH62 in Camellia sinensis”
e0“The pathogen Colletotrichum gloeosporioides causes anthracnose, a serious threat to tea trees around the world”
e2“CsNAC17 or CsbHLH62 overexpression enhanced anthracnose resistance, as well as enhanced levels of H2O2, hypersensitivity, and cell death in Nicotiana benthamiana”
Claim · model-written summary
Overexpression of CsNAC17 or CsbHLH62 enhanced levels of H2O2, hypersensitivity and cell death in Nicotiana benthamiana.
- Taxon
- Camellia sinensisCamellia sinensist0
- Treatment
- CsNAC17 or CsbHLH62 overexpressione2
- Outcome
- levels of H2O2, hypersensitivity, and cell deathe2
- Direction
- enhancede2
- Qualifier
- in Nicotiana benthamianae2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“CsNAC17 enhances resistance to Colletotrichum gloeosporioides by interacting with CsbHLH62 in Camellia sinensis”
e2“CsNAC17 or CsbHLH62 overexpression enhanced anthracnose resistance, as well as enhanced levels of H2O2, hypersensitivity, and cell death in Nicotiana benthamiana”
Claim · model-written summary
CsNAC17 regulates CsRPM1 by binding directly to its promoter.
- Taxon
- Camellia sinensisCamellia sinensist0
- Gene
- CsRPM1e3
- Outcome
- CsRPM1 is regulatede3
- Qualifier
- by binding directly to its promotere3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“CsNAC17 enhances resistance to Colletotrichum gloeosporioides by interacting with CsbHLH62 in Camellia sinensis”
e3“The NBS-LRR gene CsRPM1 is regulated by CsNAC17 by binding directly to its promoter”
Claim · model-written summary
CsbHLH62 facilitates CsNAC17 binding and increases the transcriptional activity of CsRPM1.
- Taxon
- Camellia sinensisCamellia sinensist0
- Gene
- CsbHLH62e4
- Outcome
- transcriptional activity of CsRPM1e4
- Direction
- increasese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“CsNAC17 enhances resistance to Colletotrichum gloeosporioides by interacting with CsbHLH62 in Camellia sinensis”
e4“CsbHLH62 facilitates CsNAC17’s binding and increases transcriptional activity of CsRPM1”
Claim · model-written summary
Transient silencing of CsNAC17 and CsbHLH62 in tea leaves decreased anthracnose resistance.
- Taxon
- Camellia sinensisCamellia sinensist0
- Treatment
- transient silencing of CsNAC17 and CsbHLH62e5
- Interactor
- Colletotrichum gloeosporioidesColletotrichum gloeosporioidese0pathogen
- Gene
- CsNAC17 and CsbHLH62e5
- Outcome
- resistance to anthracnosee5
- Direction
- decreasede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“CsNAC17 enhances resistance to Colletotrichum gloeosporioides by interacting with CsbHLH62 in Camellia sinensis”
e0“The pathogen Colletotrichum gloeosporioides causes anthracnose, a serious threat to tea trees around the world”
e5“transient silencing of CsNAC17 and CsbHLH62 in tea plant leaves using the virus-induced gene silencing (VIGS) system resulted in decreased resistance to anthracnose”
Claim · model-written summary
Transient overexpression of CsNAC17 and CsbHLH62 in tea leaves significantly enhanced anthracnose resistance.
- Taxon
- Camellia sinensisCamellia sinensist0
- Treatment
- transient overexpression of CsNAC17 and CsbHLH62e6
- Interactor
- Colletotrichum gloeosporioidesColletotrichum gloeosporioidese0pathogen
- Gene
- CsNAC17 and CsbHLH62e6
- Outcome
- resistance against anthracnosee6
- Direction
- enhancede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“CsNAC17 enhances resistance to Colletotrichum gloeosporioides by interacting with CsbHLH62 in Camellia sinensis”
e0“The pathogen Colletotrichum gloeosporioides causes anthracnose, a serious threat to tea trees around the world”
e6“transient overexpression of CsNAC17 and CsbHLH62 in tea leaves significantly enhanced the resistance against anthracnose”
The same questions in other papers
loss of function · Colletotrichum gloeosporioides · Camellia sinensis (black tea)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.