Abstract
Anthracnose caused by Colletotrichum gloeosporioides is a major threat to tea cultivation; however, the molecular mechanism underlying different resistance among tea cultivars remains unclear. We identified distinct expression patterns of CsMYB82 between anthracnose-resistant and susceptible varieties after infection with anthracnose from previous RNA-seq data. We further investigated the role of CsMYB82 within a lignin-associated regulatory network during anthracnose responses. We found that CsMYB1 negatively regulates CsMYB82 expression by Y1H screen. Additionally, we identified the interaction between CsMYB82 and CsbHLH48 both in vitro and in vivo. DNA-affinity purification sequencing (DAP-seq) revealed that CsMYB82 directly binds to the promoter of CsCAD4, and this binding activity is enhanced in the presence of CsbHLH48. Functional analyses indicated that overexpression of CsMYB82 or CsCAD4 in tobacco and tea leaves was associated with increased susceptibility to anthracnose, whereas transient silencing of CsMYB82 or CsCAD4 via virus-induced gene silencing (VIGS) in tea leaves resulted in reduced disease symptoms accompanied by elevated lignin accumulation. The functional analysis of CsMYB1 showed the opposite phenotype. Collectively, these results suggest that CsMYB82 participates in a transcriptional regulatory module involving CsMYB1, CsbHLH48, and CsCAD4, which modulates lignin biosynthesis and influences anthracnose responses in tea plants. This study provides mechanistic insights into the transcriptional regulation of lignin-associated defence responses and contributes to a better understanding of anthracnose resistance in tea.
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Claims 15
Claim · model-written summary
Resistant and susceptible tea varieties differed in CsMYB82 expression after infection.
- Taxon
- Camellia sinensis
Camellia sinensist0 - Gene
- CsMYB82e0
- Outcome
- distinct expression patternse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We identified distinct expression patterns of CsMYB82 between anthracnose-resistant and susceptible varieties after infection with anthracnose from previous RNA-seq data.”
t0“Camellia sinensis”
Claim · model-written summary
CsMYB1 negatively regulates CsMYB82 expression.
- Taxon
- Camellia sinensisCamellia sinensist0
- Gene
- CsMYB1e0
- Outcome
- CsMYB82 expressione0
- Direction
- negatively regulatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We found that CsMYB1 negatively regulates CsMYB82 expression by Y1H screen.”
t0“Camellia sinensis”
Claim · model-written summary
CsMYB82 interacts with CsbHLH48 in vitro and in vivo.
- Taxon
- Camellia sinensisCamellia sinensist0
- Gene
- CsMYB82e0
- Outcome
- interaction between CsMYB82 and CsbHLH48e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Additionally, we identified the interaction between CsMYB82 and CsbHLH48 both in vitro and in vivo.”
t0“Camellia sinensis”
Claim · model-written summary
CsMYB82 directly binds the CsCAD4 promoter.
- Taxon
- Camellia sinensisCamellia sinensist0
- Gene
- CsMYB82e0
- Outcome
- directly binds to the promoter of CsCAD4e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DNA-affinity purification sequencing (DAP-seq) revealed that CsMYB82 directly binds to the promoter of CsCAD4”
t0“Camellia sinensis”
Claim · model-written summary
CsbHLH48 enhances that binding activity.
- Taxon
- Camellia sinensisCamellia sinensist0
- Gene
- CsbHLH48e0
- Outcome
- binding activitye0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“and this binding activity is enhanced in the presence of CsbHLH48.”
t0“Camellia sinensis”
Claim · model-written summary
CsMYB82 overexpression increased anthracnose susceptibility in tobacco.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- overexpression of CsMYB82e0
- Interactor
- Colletotrichum gloeosporioides
Colletotrichum gloeosporioidest0pathogen - Outcome
- susceptibility to anthracnosee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Functional analyses indicated that overexpression of CsMYB82 or CsCAD4 in tobacco and tea leaves was associated with increased susceptibility to anthracnose”
t0“Colletotrichum gloeosporioides”
Claim · model-written summary
CsCAD4 overexpression increased anthracnose susceptibility in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- CsCAD4e0
- Interactor
- Colletotrichum gloeosporioidesColletotrichum gloeosporioidest0pathogen
- Outcome
- susceptibility to anthracnosee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Functional analyses indicated that overexpression of CsMYB82 or CsCAD4 in tobacco and tea leaves was associated with increased susceptibility to anthracnose”
t0“Colletotrichum gloeosporioides”
Claim · model-written summary
CsMYB82 overexpression increased anthracnose susceptibility in tea leaves.
- Taxon
- teae0
- Organ / stage
- leavese0
- Treatment
- overexpression of CsMYB82e0
- Interactor
- Colletotrichum gloeosporioidesColletotrichum gloeosporioidest0pathogen
- Outcome
- susceptibility to anthracnosee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Functional analyses indicated that overexpression of CsMYB82 or CsCAD4 in tobacco and tea leaves was associated with increased susceptibility to anthracnose”
t0“Colletotrichum gloeosporioides”
Claim · model-written summary
CsCAD4 overexpression increased anthracnose susceptibility in tea leaves.
- Taxon
- teae0
- Organ / stage
- leavese0
- Treatment
- CsCAD4e0
- Interactor
- Colletotrichum gloeosporioidesColletotrichum gloeosporioidest0pathogen
- Outcome
- susceptibility to anthracnosee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Functional analyses indicated that overexpression of CsMYB82 or CsCAD4 in tobacco and tea leaves was associated with increased susceptibility to anthracnose”
t0“Colletotrichum gloeosporioides”
Claim · model-written summary
Silencing CsMYB82 reduced disease symptoms in tea leaves.
- Taxon
- teae0
- Treatment
- transient silencing of CsMYB82e0
- Interactor
- Colletotrichum gloeosporioidesColletotrichum gloeosporioidest0pathogen
- Outcome
- disease symptomse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“whereas transient silencing of CsMYB82 or CsCAD4 via virus-induced gene silencing (VIGS) in tea leaves resulted in reduced disease symptoms accompanied by elevated lignin accumulation.”
t0“Colletotrichum gloeosporioides”
Claim · model-written summary
Silencing CsCAD4 reduced disease symptoms in tea leaves.
- Taxon
- teae0
- Treatment
- CsCAD4e0
- Interactor
- Colletotrichum gloeosporioidesColletotrichum gloeosporioidest0pathogen
- Outcome
- disease symptomse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“whereas transient silencing of CsMYB82 or CsCAD4 via virus-induced gene silencing (VIGS) in tea leaves resulted in reduced disease symptoms accompanied by elevated lignin accumulation.”
t0“Colletotrichum gloeosporioides”
Claim · model-written summary
Silencing CsMYB82 elevated lignin accumulation.
Not reportedComparator
Verbatim
e0“whereas transient silencing of CsMYB82 or CsCAD4 via virus-induced gene silencing (VIGS) in tea leaves resulted in reduced disease symptoms accompanied by elevated lignin accumulation.”
Claim · model-written summary
Silencing CsCAD4 elevated lignin accumulation.
Not reportedComparator
Verbatim
e0“whereas transient silencing of CsMYB82 or CsCAD4 via virus-induced gene silencing (VIGS) in tea leaves resulted in reduced disease symptoms accompanied by elevated lignin accumulation.”
Claim · model-written summary
CsMYB1 functional analysis showed the opposite phenotype.
- Taxon
- Camellia sinensisCamellia sinensist0
- Gene
- CsMYB1e0
- Outcome
- opposite phenotypee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The functional analysis of CsMYB1 showed the opposite phenotype.”
t0“Camellia sinensis”
Claim · model-written summary
The CsMYB82 regulatory module modulates lignin biosynthesis and anthracnose responses.
Verbatim
e0“Collectively, these results suggest that CsMYB82 participates in a transcriptional regulatory module involving CsMYB1, CsbHLH48, and CsCAD4, which modulates lignin biosynthesis and influences anthracnose responses in tea plants.”
The same questions in other papers
loss of function · Colletotrichum gloeosporioides · Camellia sinensis (black tea)Positive and negative results
1 in the opposite direction
- negative
loss of function · molecular: metabolites · Camellia sinensis (black tea)Positive and negative results
3 papers in the same direction as this paper · 9 in the opposite direction
- negative
- negative
- negative
9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.