Abstract
The promotion of vegetative branching in plants after topping has been observed without a clear understanding of the underlying mechanism. This study aimed to investigate the role of isopentenyl transferase ( IPT ) and cytokinins in the regulation of vegetative branching in cotton ( Gossypium hirsutum L.), hypothesizing that they play key roles in this process. Plant topping was implemented at peak flowering stage in field-grown cotton, and seedcotton yield, yield components, vegetative branching, fruiting, and cytokinin levels were examined over two consecutive years. The results showed that plant topping significantly enhanced the growth and development of vegetative branches, as evidenced by increased biomass, leaf area, and fruiting of vegetative branches, along with an accumulation of cytokinin accumulation at the tips of vegetative branches. This led to a 10.3 % rise in boll density and an 11.4 % increase in seedcotton yield compared with the non-topped control. The analysis of RNA-seq and qRT-PCR data revealed significant differences in the expression patterns of IPT genes, suggesting their importance in regulating the growth and development of vegetative branches. Furthermore, examing the IPT gene families in diploid and tetraploid cotton species identified a total of 8, 8, 16, and 16 IPT genes, categorized into 6 groups. Prediction of cis-acting elements of GhIPT gene family promoters and analysis of their expression profiles demonstrated the involvement of GhIPT genes in multiple plant growth pathways with specific spatio-temporal expression. The findings underline that plant topping enhances cytokinins accumulation at vegetative branch tips by up-regulating IPT genes, leading to increased vegetative branching, fruiting, and ultimately, seedcotton yield. This study provides valuable insights into enhancing cotton yield formation through the regulation of growth and development of vegetative branches by GhIPTs and offers a crucial reference for further investigations into the functions of IPT genes. • Plant topping at peak flowering boost vegetative branch growth in cotton. • Topping increases expression of isopentenyl transferase genes in branches. • Topping enhances cytokinin accumulation through gene expression. • Raised cytokinin accumulation led to enhanced vegetative branching. • Topping enhances seedcotton yield through enhanced vegetative branching.
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Claims 7
Claim · model-written summary
Topping increased vegetative branch biomass, leaf area and fruiting.
- Taxon
- cotton
Gossypium hirsutumt0 - Treatment
- plant toppinge0
- Outcome
- biomass, leaf area, and fruiting of vegetative branchese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plant topping significantly enhanced the growth and development of vegetative branches, as evidenced by increased biomass, leaf area, and fruiting of vegetative branches”
t0“cotton”
Claim · model-written summary
Topping increased cytokinin accumulation at vegetative branch tips.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- tips of vegetative branchese0
- Treatment
- toppingt0
- Outcome
- cytokinin accumulatione0
- Direction
- accumulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“along with an accumulation of cytokinin accumulation at the tips of vegetative branches”
t0“Genome-wide identification of isopentenyl transferase genes in cotton and their roles in regulating vegetative branching after topping”
Claim · model-written summary
Topping increased cotton boll density by 10.3% versus non-topped control.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- toppingt0
- Outcome
- boll densitye0
- Direction
- risee0
- Value
- 10.3 %e0
- Comparator
- non-topped controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This led to a 10.3 % rise in boll density and an 11.4 % increase in seedcotton yield compared with the non-topped control”
t0“topping”
Claim · model-written summary
Topping increased seedcotton yield by 11.4% versus non-topped control.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- toppingt0
- Outcome
- seedcotton yielde0
- Direction
- increasee0
- Value
- 11.4 %e0
- Comparator
- non-topped controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This led to a 10.3 % rise in boll density and an 11.4 % increase in seedcotton yield compared with the non-topped control”
t0“topping”
Claim · model-written summary
IPT genes showed significantly different expression patterns.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- IPT genese0
- Outcome
- expression patterns of IPT genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“significant differences in the expression patterns of IPT genes”
t0“cotton”
Claim · model-written summary
Diploid and tetraploid cotton species had 8, 8, 16 and 16 IPT genes across six groups.
- Taxon
- cottonGossypium hirsutume0
- Gene
- IPT genese0
- Outcome
- IPT genese0
- Value
- 8, 8, 16, and 16e0
- Qualifier
- 6 groupse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“diploid and tetraploid cotton species identified a total of 8, 8, 16, and 16 IPT genes, categorized into 6 groups”
Claim · model-written summary
GhIPT expression profiles and promoter elements implicated the genes in multiple growth pathways.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- GhIPT genese0
- Outcome
- multiple plant growth pathwayse0
- Qualifier
- specific spatio-temporal expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Prediction of cis-acting elements of GhIPT gene family promoters and analysis of their expression profiles demonstrated the involvement of GhIPT genes in multiple plant growth pathways with specific spatio-temporal expression”
t0“cotton”
The same questions in other papers
pruning · yield · Gossypium hirsutum (cotton)Results differ
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- positive
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See all 76 papers →pruning · growth · Gossypium hirsutum (cotton)Positive and negative results
20 papers in the same direction as this paper · 6 in the opposite direction
- negative
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See all 27 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.