Abstract
Gamma irradiation-based mutant creation is one of the most important methods for rice plant mutagenesis breeding and molecular biology research. Although median lethal dose irradiation severely damages rice seedlings, applying brassinolide (BR) can increase the survival rate of irradiated seedlings. In this study, we investigated the effects of soaking seeds in solutions containing different BR concentrations (0.001, 0.01, 0.1, 1.0, and 5.0 μmol/L) and then spraying the resulting seedlings twice with 0.1 μmol/L BR. The combined BR treatments markedly decreased the superoxide anion (O2•−), hydrogen peroxide (H2O2), and malondialdehyde contents but increased the chlorophyll content. An appropriate BR treatment of gamma-irradiated samples substantially increased the activities of the antioxidant enzymes superoxide dismutase, peroxidase, and ascorbate peroxidase as well as the proline, ascorbic acid, and glutathione contents in rice seedling shoots. The BR treatment also promoted the growth of seedlings derived from irradiated seeds and increased the shoot and root fresh and dry weights. Most notably, soaking seeds in 0.01 or 0.1 μmol/L BR solutions and then spraying seedlings twice with 0.1 μmol/L BR significantly increased the final seedling survival rate and decreased mutant loss. The study results suggest that exogenous BR treatments can protect rice seedlings from gamma irradiation stress by enhancing antioxidant metabolism.
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Claims 9
Claim · model-written summary
The combined BR treatments markedly decreased the superoxide anion (O2•−), hydrogen peroxide (H2O2), and malondialdehyde contents
- Taxon
- rice
Oryza sativat0 - Treatment
- combined BR treatmentse0
- Outcome
- superoxide anion (O2•−), hydrogen peroxide (H2O2), and malondialdehyde contentse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The combined BR treatments markedly decreased the superoxide anion (O2•−), hydrogen peroxide (H2O2), and malondialdehyde contents”
t0“Rice Seedlings”
Claim · model-written summary
increased the chlorophyll content
- Taxon
- riceOryza sativat0
- Treatment
- BR treatmentse1
- Outcome
- chlorophyll contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased the chlorophyll content”
t0“Rice Seedlings”
e1“combined BR treatments”
Claim · model-written summary
increased the activities of the antioxidant enzymes superoxide dismutase, peroxidase, and ascorbate peroxidase
- Taxon
- riceOryza sativae1
- Treatment
- BR treatmente2
- Outcome
- activities of the antioxidant enzymes superoxide dismutase, peroxidase, and ascorbate peroxidasee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased the activities of the antioxidant enzymes superoxide dismutase, peroxidase, and ascorbate peroxidase”
e1“rice seedling shoots”
e2“An appropriate BR treatment of gamma-irradiated samples”
Claim · model-written summary
proline, ascorbic acid, and glutathione contents in rice seedling shoots
- Taxon
- riceOryza sativae0
- Treatment
- BR treatmente1
- Outcome
- proline, ascorbic acid, and glutathione contentse0
- Direction
- increasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“proline, ascorbic acid, and glutathione contents in rice seedling shoots”
e1“An appropriate BR treatment of gamma-irradiated samples”
e2“increased the activities of the antioxidant enzymes”
Claim · model-written summary
The BR treatment also promoted the growth of seedlings derived from irradiated seeds
- Taxon
- riceOryza sativat0
- Treatment
- BR treatmente0
- Outcome
- growth of seedlings derived from irradiated seedse0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The BR treatment also promoted the growth of seedlings derived from irradiated seeds”
t0“Rice Seedlings”
Claim · model-written summary
increased the shoot and root fresh and dry weights
- Taxon
- riceOryza sativat0
- Treatment
- BR treatmente1
- Outcome
- shoot and root fresh and dry weightse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased the shoot and root fresh and dry weights”
t0“Rice Seedlings”
e1“The BR treatment also promoted”
Claim · model-written summary
soaking seeds in 0.01 or 0.1 μmol/L BR solutions and then spraying seedlings twice with 0.1 μmol/L BR significantly increased the final seedling survival rate
- Taxon
- riceOryza sativat0
- Treatment
- soaking seeds in 0.01 or 0.1 μmol/L BR solutions and then spraying seedlings twice with 0.1 μmol/L BRe0
- Outcome
- final seedling survival ratee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soaking seeds in 0.01 or 0.1 μmol/L BR solutions and then spraying seedlings twice with 0.1 μmol/L BR significantly increased the final seedling survival rate”
t0“Rice Seedlings”
Claim · model-written summary
soaking seeds in 0.01 or 0.1 μmol/L BR solutions and then spraying seedlings twice with 0.1 μmol/L BR significantly increased the final seedling survival rate and decreased mutant loss
- Taxon
- riceOryza sativat0
- Treatment
- soaking seeds in 0.01 or 0.1 μmol/L BR solutions and then spraying seedlings twice with 0.1 μmol/L BRe0
- Outcome
- mutant losse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soaking seeds in 0.01 or 0.1 μmol/L BR solutions and then spraying seedlings twice with 0.1 μmol/L BR significantly increased the final seedling survival rate and decreased mutant loss”
t0“Rice Seedlings”
Claim · model-written summary
exogenous BR treatments can protect rice seedlings from gamma irradiation stress by enhancing antioxidant metabolism
- Taxon
- riceOryza sativae0
- Treatment
- exogenous BR treatmentse0
- Outcome
- protect rice seedlings from gamma irradiation stresse0
- Qualifier
- by enhancing antioxidant metabolisme0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous BR treatments can protect rice seedlings from gamma irradiation stress by enhancing antioxidant metabolism”
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