Abstract
Background: Bouquet is a crucial characteristic indicative of wine quality that develops during the aging stage. The cytochrome P450 VvCYP76F14 multi-functionally catalyzes linalool into (E)-8-hydroxylinalool, (E)-8-oxolinalool, and (E)-8-carboxylinalool, which are direct precursors for wine bouquet. Wine bouquet was closely related to VvCYP76F14 activities. Method: The VvCYP76F14 genes were cloned from five wine grape varieties using a homologous cloning method. The variation in residues of VvCYP76F14s were assessed by multiple alignment of amino acid sequences. Functional studies were implemented by in vitro enzyme activity and transient over-expression systems. Results: D299T variation was observed in VvCYP76F14s of ‘Yantai 2-2-08’, ‘Yantai 2-2-19’, and ‘Yantai 2-3-37’ offspring lines, which was correlated with the decreased content of wine bouquet precursors of (E)-8-hydroxylinalool, (E)-8-oxolinalool, and (E)-8-carboxylinalool, respectively. Notably, the key amino acid residue D299 was located at the phase 0 intron positions of VvCYP76F14 genes isolated from distinct wine grape varieties or offspring lines, respectively. Notably, VvCYP76F14s of the ‘Yantai2-2-08’, ‘Yantai 2-2-19’, and ‘Yantai 2-3-37’ mutant lines exhibited lower in vitro enzymatic activities than those of ‘L35’ and ‘Merlot’. In addition, the transient expression of VvCYP76F14 cloned from ‘L35’ and ‘Merlot’ restored the levels of wine bouquet precursors in berries of three D299T mutant lines, respectively, whereas VvCYP76F14 cloned from D299T mutant lines failed. Conclusions: D299T variation was observed in three offspring lines and D299T mutation in VvCYP76F14 led to the decrease in wine bouquet precursor contents. VvCYP76F14 was implicated in the regulation of wine bouquet precursors in wine grapes.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
D299T variation in VvCYP76F14 of three Yantai offspring lines was correlated with decreased content of the wine bouquet precursors (E)-8-hydroxylinalool, (E)-8-oxolinalool and (E)-8-carboxylinalool.
- Taxon
- Wine Grape
Vitis viniferat0 - Material
- Yantai 2-2-19e0
- Gene
- VvCYP76F14e0
- Outcome
- content of wine bouquet precursorse0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“D299T variation was observed in VvCYP76F14s of ‘Yantai 2-2-08’, ‘Yantai 2-2-19’, and ‘Yantai 2-3-37’ offspring lines, which was correlated with the decreased content of wine bouquet precursors”
t0“Wine Grape”
Claim · model-written summary
The key amino acid residue D299 was located at the phase 0 intron positions of VvCYP76F14 genes from distinct wine grape varieties or offspring lines.
- Taxon
- Wine GrapeVitis viniferat0
- Gene
- VvCYP76F14e0
- Outcome
- located at the phase 0 intron positionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the key amino acid residue D299 was located at the phase 0 intron positions of VvCYP76F14 genes”
t0“Wine Grape”
Claim · model-written summary
VvCYP76F14 of the Yantai mutant lines showed lower in vitro enzymatic activities than that of L35 and Merlot.
- Taxon
- Wine GrapeVitis viniferat0
- Material
- Yantai 2-2-19e0
- Gene
- VvCYP76F14e0
- Outcome
- in vitro enzymatic activitiese0
- Direction
- lowere0
- Comparator
- ‘L35’ and ‘Merlot’e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VvCYP76F14s of the ‘Yantai2-2-08’, ‘Yantai 2-2-19’, and ‘Yantai 2-3-37’ mutant lines exhibited lower in vitro enzymatic activities than those of ‘L35’ and ‘Merlot’”
t0“Wine Grape”
Claim · model-written summary
Transient expression of VvCYP76F14 cloned from L35 and Merlot restored the levels of wine bouquet precursors in berries of the three D299T mutant lines, whereas VvCYP76F14 cloned from D299T mutant lines failed.
- Taxon
- Wine GrapeVitis viniferat0
- Organ / stage
- berriese0
- Treatment
- transient expression of VvCYP76F14 cloned from ‘L35’ and ‘Merlot’e0
- Gene
- VvCYP76F14e0
- Outcome
- levels of wine bouquet precursorse0
- Direction
- restorede0
- Comparator
- VvCYP76F14 cloned from D299T mutant linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transient expression of VvCYP76F14 cloned from ‘L35’ and ‘Merlot’ restored the levels of wine bouquet precursors in berries of three D299T mutant lines, respectively, whereas VvCYP76F14 cloned from D299T mutant lines failed”
t0“Wine Grape”
Claim · model-written summary
The D299T mutation in VvCYP76F14 led to a decrease in wine bouquet precursor contents.
- Taxon
- Wine GrapeVitis viniferat0
- Gene
- VvCYP76F14e0
- Outcome
- wine bouquet precursor contentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“D299T mutation in VvCYP76F14 led to the decrease in wine bouquet precursor contents”
t0“Wine Grape”
Claim · model-written summary
VvCYP76F14 was implicated in the regulation of wine bouquet precursors in wine grapes.
- Taxon
- wine grapesVitis viniferae0
- Gene
- VvCYP76F14e0
- Outcome
- wine bouquet precursorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VvCYP76F14 was implicated in the regulation of wine bouquet precursors in wine grapes”