Paper · 2024 · Pertanika journal of tropical agricultural science

Growth Response and Gene Expression Analysis of Chili Pepper (Capsicum annuum L.) Plant Dehydrin Against Salt Stress and Drought In vitro

Elly Syafriani; Widhi Dyah Sawitri; Ersa Nur Syafia

3
claims
4
verbatim quotes
0
citations
2
research areas
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Open access · cc-by-nc-nd

Abstract

The need for plants resistant to abiotic stress now and in the future is predicted to be very high. It is related to extreme climate change and converting agricultural land into residential and industrial land. As Indonesia’s national strategic commodity, chili peppers require special attention when assembling chili peppers resistant to salinity and drought stress. New varieties of chili pepper plants resistant to saline and drought can be obtained through unconventional breeding (overexpression of the dehydrin gene). As a first step in assembling saline and drought-resistant chili plants, growth response and dehydrin gene expression tests were carried out from explants of chili pepper plants of the TM999 variety in vitro on salt and drought treatment media. This study aims to obtain information on the initial response to the growth and expression of the dehydrin gene from chili pepper plants of the TM999 variety before further research is carried out to increase the expression of the dehydrin gene through a molecular approach. The method used in this study is a complete randomized design with two treatments: Sodium chloride (NaCl) and polyethylene glycol (PEG-6000). The results obtained in this study showed that chili pepper varieties TM999 were more tolerant of drought stress than salinity based on several growth response data in both treatments. The analysis of dehydrin gene expression in both treatments showed that the gene expression was strongly influenced by the two strokes given. NaCl and PEG-6000 treatments increased the dehydrin gene expression of chili pepper plants grown in vitro.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 3

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Chili pepper TM999 was more tolerant of drought stress than of salinity, based on growth response data

Taxon
chili pepperCapsicume1
Material
TM999e1
Treatment
drought stresse1
Outcome
growth response datae1
Direction
more tolerante1
Comparator
salinitye1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum
Capsicum · Taxon · picture of the genus (one of its species)Public domain · Wikimedia Commons ↗

Verbatim

e1

“chili pepper varieties TM999 were more tolerant of drought stress than salinity based on several growth response data in both treatments”

✓ located verbatim in the source · abstract, characters 1233–1369 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

The two stress treatments strongly influenced dehydrin gene expression in chili pepper

Taxon
chili pepperCapsicume0
Treatment
two strokes givene2
Gene
dehydrine2
Outcome
gene expressione2

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum
Capsicum · Taxon · picture of the genus (one of its species)Public domain · Wikimedia Commons ↗

Verbatim

e0

“growth response and dehydrin gene expression tests were carried out from explants of chili pepper plants of the TM999 variety in vitro on salt and drought treatment media”

✓ located verbatim in the source · abstract, characters 597–767 · show in text ↑
e2

“The analysis of dehydrin gene expression in both treatments showed that the gene expression was strongly influenced by the two strokes given”

✓ located verbatim in the source · abstract, characters 1371–1511 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

NaCl and PEG-6000 treatments increased dehydrin gene expression in chili pepper grown in vitro

Taxon
chili pepper plantsCapsicume3
Treatment
NaCl and PEG-6000e3
Gene
dehydrine3
Outcome
dehydrin gene expressione3
Direction
increasede3
Setting
in vitroe3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum
Capsicum · Taxon · picture of the genus (one of its species)Public domain · Wikimedia Commons ↗

Verbatim

e3

“NaCl and PEG-6000 treatments increased the dehydrin gene expression of chili pepper plants grown in vitro”

✓ located verbatim in the source · abstract, characters 1513–1618 · show in text ↑

drought · molecular: expression · Capsicum (peppers)Positive and negative results

3 papers: 2 positive, 1 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
See all 3 papers →

drought · growth · Capsicum (peppers)Positive and negative results

9 papers: 3 positive, 6 negative or no effect

2 papers in the same direction as this paper · 6 in the opposite direction

  1. no effect
  2. negative
  3. negative

5 papers more in this group

See all 9 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.