Abstract
Plants require adequate water for growth, development, and reproduction. Peptides play a key role in plant growth and development and act in a similar manner to plant hormones. However, only a few peptides have been identified to play a role in abiotic stress tolerance in potato. In this study, we identified fourteen members of the epidermal patterning factor (EPF) family in potato, which were designated as StEPF1-14 according to their chromosomal locations. We also conducted a comprehensive analysis of their chromosomal distribution, gene structures, physicochemical properties, phylogenetic relationships, and tissue-specific expression patterns. RT-qPCR analysis revealed that the StEPF4 gene is significantly induced by drought stress, suggesting its potential role as a negative regulator in the plant’s response to drought. Furthermore, multiple cis-regulatory elements associated with drought-responsive regulation were identified within the promoter region of the StEPF genes. Here, we isolated an EPF secreted Cys-rich small peptide StEPF4 from ‘Atlantic’ and explored its mechanism in plant response to drought stress. We found that StEPF4 was greatly induced by dehydration treatment in potato. To investigate its potential biological functions, StEPF4 was knocked down in potato. The StEPF4 knocked down lines (KdStEPF4) significantly decreased stomatal density, resulting in a decrease in the transpiration rate. KdStEPF4 lines maintained a higher photosynthetic rate and lowered the water loss rate of leaves compared with the control, resulting in increased drought resistance. Taken together, this study provides detailed information about StEPFs, and our findings also show that StEPF4 plays an essential role in regulating drought resistance by reducing stomatal density in potato.
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Claims 11
Claim · model-written summary
Fourteen EPF family members were identified in potato.
- Taxon
- potato
Solanum tuberosume1 - Outcome
- members of the epidermal patterning factor (EPF) familye1
- Value
- fourteene1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gene Family in Potato”
e1“we identified fourteen members of the epidermal patterning factor (EPF) family in potato”
Claim · model-written summary
StEPF4 is significantly induced by drought stress in potato.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- drought stresse1
- Gene
- StEPF4e1
- Outcome
- StEPF4 genee1
- Direction
- inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gene Family in Potato”
e1“RT-qPCR analysis revealed that the StEPF4 gene is significantly induced by drought stress”
Claim · model-written summary
StEPF4 may act as a negative regulator of the drought response in potato.
- Taxon
- PotatoSolanum tuberosumt0
- Gene
- StEPF4e1
- Outcome
- induced by drought stresse1
- Qualifier
- negative regulatore1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gene Family in Potato”
e1“RT-qPCR analysis revealed that the StEPF4 gene is significantly induced by drought stress, suggesting its potential role as a negative regulator”
Claim · model-written summary
Multiple cis-regulatory elements associated with drought regulation were identified in the StEPF promoter regions.
- Taxon
- PotatoSolanum tuberosumt0
- Gene
- StEPF genese1
- Outcome
- cis-regulatory elementse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gene Family in Potato”
e1“multiple cis-regulatory elements associated with drought-responsive regulation were identified within the promoter region of the StEPF genes”
Claim · model-written summary
StEPF4 is greatly induced by dehydration treatment in potato.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- dehydration treatmente1
- Gene
- StEPF4e1
- Outcome
- StEPF4e1
- Direction
- greatly inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We found that StEPF4 was greatly induced by dehydration treatment in potato”
Claim · model-written summary
StEPF4 knockdown significantly decreased stomatal density in potato.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- StEPF4 knocked down linese1
- Outcome
- stomatal densitye1
- Direction
- decreasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gene Family in Potato”
e1“The StEPF4 knocked down lines (KdStEPF4) significantly decreased stomatal density, resulting in a decrease in the transpiration rate”
Claim · model-written summary
StEPF4 knockdown decreased transpiration rate in potato.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- StEPF4 knocked down linese1
- Outcome
- transpiration ratee1
- Direction
- decreasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gene Family in Potato”
e1“The StEPF4 knocked down lines (KdStEPF4) significantly decreased stomatal density, resulting in a decrease in the transpiration rate”
Claim · model-written summary
StEPF4 knockdown lines maintained a higher photosynthetic rate than the control.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- KdStEPF4 linese1
- Outcome
- photosynthetic ratee1
- Direction
- highere1
- Comparator
- controle1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gene Family in Potato”
e1“KdStEPF4 lines maintained a higher photosynthetic rate and lowered the water loss rate of leaves compared with the control, resulting in increased drought resistance”
Claim · model-written summary
StEPF4 knockdown lines lowered leaf water loss rate compared with the control.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- KdStEPF4 linese1
- Outcome
- water loss ratee1
- Direction
- lowerede1
- Comparator
- controle1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gene Family in Potato”
e1“KdStEPF4 lines maintained a higher photosynthetic rate and lowered the water loss rate of leaves compared with the control, resulting in increased drought resistance”
Claim · model-written summary
StEPF4 knockdown lines showed increased drought resistance compared with the control.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- KdStEPF4 linese1
- Outcome
- drought resistancee1
- Direction
- increasede1
- Comparator
- controle1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gene Family in Potato”
e1“KdStEPF4 lines maintained a higher photosynthetic rate and lowered the water loss rate of leaves compared with the control, resulting in increased drought resistance”
Claim · model-written summary
The authors conclude that StEPF4 regulates drought resistance in potato by reducing stomatal density.
- Taxon
- PotatoSolanum tuberosumt0
- Gene
- StEPF4e1
- Outcome
- drought resistancee1
- Qualifier
- by reducing stomatal densitye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gene Family in Potato”
e1“StEPF4 plays an essential role in regulating drought resistance by reducing stomatal density in potato”