Abstract
Virus-derived small interfering RNAs (vsiRNAs) play an important role in viral infection by regulating the expression of host genes. At present, research on the regulation of plant primary metabolic pathways by vsiRNAs is very limited. TvsiRNA24 derived from tobacco curly shoot virus (TbCSV) was amplified by reverse transcription polymerase chain reaction, and its target gene NbTPI (triosephosphate isomerase) was verified using reverse transcription quantitative polymerase chain reaction and GFP fluorescence observation. The effect of the interaction between TvsiRNA24 and NbTPI on TbCSV infection was analyzed by virus mediated, genetic transformation, western blotting, and quantitative detection. The expression of TvsiRNA24 retards the growth of Nicotiana benthamiana and enhances TbCSV accumulation within N. benthamiana. The overexpression of NbTPI attenuates the accumulation of TbCSV, and the silencing of NbTPI leads to the growth retardation of N. benthamiana and intensifies symptoms post-TbCSV infection. Moreover, the expression of some genes related to photosynthesis, primary metabolism and immune response is regulated by NbTPI. Our results unveil the specific role of TvsiRNA24-NbTPI in the pathogenicity of TbCSV, resulting in hindrance to plant growth and facilitation of viral infection. The identification of this regulatory pathway provides valuable insights that can be utilized to devise novel antiviral approaches targeting the reduction of viral pathogenicity.
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Claims 7
Claim · model-written summary
NbTPI was verified as the target of virus-derived TvsiRNA24.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianat0 - Interactor
- tobacco curly shoot virus (TbCSV)Tobacco curly shoot viruse0pathogen
- Gene
- NbTPIe0
- Outcome
- target gene NbTPIe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TvsiRNA24 derived from tobacco curly shoot virus (TbCSV) was amplified by reverse transcription polymerase chain reaction, and its target gene NbTPI (triosephosphate isomerase) was verified using reverse transcription quantitative polymerase chain reaction and GFP fluorescence observation.”
t0“Nicotiana benthamiana”
Claim · model-written summary
TvsiRNA24 expression retarded N. benthamiana growth.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Treatment
- expression of TvsiRNA24e0
- Interactor
- TbCSVe0pathogen
- Gene
- TvsiRNA24e0
- Outcome
- growth of Nicotiana benthamianae0
- Direction
- retardse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of TvsiRNA24 retards the growth of Nicotiana benthamiana and enhances TbCSV accumulation within N. benthamiana.”
Claim · model-written summary
TvsiRNA24 expression increased TbCSV accumulation in N. benthamiana.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Treatment
- expression of TvsiRNA24e0
- Interactor
- TbCSVe0pathogen
- Gene
- TvsiRNA24e0
- Outcome
- TbCSV accumulatione0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of TvsiRNA24 retards the growth of Nicotiana benthamiana and enhances TbCSV accumulation within N. benthamiana.”
Claim · model-written summary
NbTPI overexpression reduced TbCSV accumulation.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- overexpression of NbTPIe0
- Interactor
- TbCSVe0pathogen
- Gene
- NbTPIe0
- Outcome
- accumulation of TbCSVe0
- Direction
- attenuatese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The overexpression of NbTPI attenuates the accumulation of TbCSV, and the silencing of NbTPI leads to the growth retardation of N. benthamiana and intensifies symptoms post-TbCSV infection.”
Claim · model-written summary
NbTPI silencing retarded N. benthamiana growth.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- silencing of NbTPIe0
- Interactor
- TbCSVe0pathogen
- Gene
- NbTPIe0
- Outcome
- growth retardation of N. benthamianae0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The overexpression of NbTPI attenuates the accumulation of TbCSV, and the silencing of NbTPI leads to the growth retardation of N. benthamiana and intensifies symptoms post-TbCSV infection.”
Claim · model-written summary
NbTPI silencing intensified symptoms after TbCSV infection.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- silencing of NbTPIe0
- Interactor
- TbCSVe0pathogen
- Gene
- NbTPIe0
- Outcome
- symptoms post-TbCSV infectione0
- Direction
- intensifiese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The overexpression of NbTPI attenuates the accumulation of TbCSV, and the silencing of NbTPI leads to the growth retardation of N. benthamiana and intensifies symptoms post-TbCSV infection.”
Claim · model-written summary
NbTPI regulated expression of genes related to photosynthesis, primary metabolism, and immunity.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- NbTPIe0
- Outcome
- expression of some genes related to photosynthesis, primary metabolism and immune responsee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Moreover, the expression of some genes related to photosynthesis, primary metabolism and immune response is regulated by NbTPI.”
t0“Nicotiana benthamiana”
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