Abstract
Here, we conducted physiological, global DNA methylation, and transcriptome assays on alfalfa ( Medicago sativa L.) exposed to recycle drought stress to investigate stress memory and the response of subsequent drought stress and recovery. Our findings revealed a distinct memory response characterized by significant alterations in DNA cytosine methylation patterns across the genome. Notably, seedlings that underwent drought stress training exhibited enhanced growth and vitality, displaying greener and healthier traits than the control group after rewatering. Jasmonic acid and cytokinin levels were improved in drought stress-trained alfalfa, emphasizing these two hormones play a key role in drought memory responses. Furthermore, the upregulation of RuBisCO, peroxidase, and superoxide dismutase in drought stress-trained alfalfa reinforces the enhanced stress tolerance acquired through drought stress training. Differential DNA methylation during drought training mainly facilitates the selective expression of energy metabolism-related genes. A transcriptomic analysis revealed a notable promotion of energy metabolism, photosynthesis, and nitrogen metabolism in alfalfa enabling individuals to endure subsequent drought stress following recurrent drought training. Post-rewatering, alfalfa underwent a metabolic shift from energy metabolism to lipid catabolism to generate energy to cope with drought stress and promote growth. This study offers valuable understanding into the mechanisms governing the formation of stress memory in alfalfa exposed to drought, presenting potential strategies for improving plant plasticity and productivity under water scarcity induced by climate change. • Stress memory confirmed in alfalfa under recurrent drought. • Drought training-induced DNA methylation enhances selective expression of energy metabolism genes. • Drought trained alfalfa shows enhanced tolerance and recovery abilities. • Trained alfalfa upregulates energy, nitrogen, and photosynthesis metabolism. • Upon re-watering, the energy supply shifted to lipid catabolism, promoting growth.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
Drought stress-trained seedlings showed enhanced growth and vitality compared with the control group after rewatering.
- Taxon
- alfalfa Medicago sativa L.
Medicago sativat0 - Treatment
- drought stress traininge1
- Outcome
- growth and vitalitye1
- Direction
- enhancede1
- Qualifier
- greener and healthier traitse1
- Comparator
- control groupe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“alfalfa Medicago sativa L.”
e1“seedlings that underwent drought stress training exhibited enhanced growth and vitality, displaying greener and healthier traits than the control group after rewatering”
Claim · model-written summary
Jasmonic acid and cytokinin levels were improved in drought stress-trained alfalfa.
- Taxon
- alfalfa Medicago sativa L.Medicago sativat0
- Treatment
- drought stress-trainede2
- Outcome
- Jasmonic acid and cytokinin levelse2
- Direction
- improvede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“alfalfa Medicago sativa L.”
e2“Jasmonic acid and cytokinin levels were improved in drought stress-trained alfalfa”
Claim · model-written summary
Jasmonic acid and cytokinin may play a key role in drought memory responses of alfalfa.
- Taxon
- alfalfa Medicago sativa L.Medicago sativat0
- Compound
- Jasmonic acid and cytokinine2
- Outcome
- drought memory responsese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“alfalfa Medicago sativa L.”
e2“Jasmonic acid and cytokinin levels were improved in drought stress-trained alfalfa”
e3“emphasizing these two hormones play a key role in drought memory responses”
Claim · model-written summary
Drought stress-trained alfalfa showed upregulation of RuBisCO, peroxidase and superoxide dismutase.
- Taxon
- alfalfa Medicago sativa L.Medicago sativat0
- Treatment
- drought stress-trainede4
- Outcome
- RuBisCO, peroxidase, and superoxide dismutasee4
- Direction
- upregulatione4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“alfalfa Medicago sativa L.”
e4“the upregulation of RuBisCO, peroxidase, and superoxide dismutase in drought stress-trained alfalfa”
Claim · model-written summary
Differential DNA methylation during drought training mainly facilitates selective expression of energy metabolism-related genes in alfalfa.
- Taxon
- alfalfa Medicago sativa L.Medicago sativat0
- Gene
- energy metabolism-related genese5
- Outcome
- selective expressione5
- Qualifier
- Differential DNA methylation during drought traininge5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“alfalfa Medicago sativa L.”
e5“Differential DNA methylation during drought training mainly facilitates the selective expression of energy metabolism-related genes”
Claim · model-written summary
Drought stress memory caused significant alterations in DNA cytosine methylation patterns across the alfalfa genome.
- Taxon
- alfalfa Medicago sativa L.Medicago sativat0
- Outcome
- DNA cytosine methylation patternse6
- Qualifier
- across the genomee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“alfalfa Medicago sativa L.”
e6“significant alterations in DNA cytosine methylation patterns across the genome”
Claim · model-written summary
Recurrent drought training promoted energy metabolism, photosynthesis and nitrogen metabolism in alfalfa.
- Taxon
- alfalfa Medicago sativa L.Medicago sativat0
- Treatment
- recurrent drought traininge7
- Outcome
- energy metabolism, photosynthesis, and nitrogen metabolisme7
- Direction
- promotione7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“alfalfa Medicago sativa L.”
e7“A transcriptomic analysis revealed a notable promotion of energy metabolism, photosynthesis, and nitrogen metabolism in alfalfa enabling individuals to endure subsequent drought stress following recurrent drought training”
Claim · model-written summary
After rewatering, alfalfa shifted metabolism from energy metabolism to lipid catabolism.
- Taxon
- alfalfa Medicago sativa L.Medicago sativat0
- Outcome
- metabolic shift from energy metabolism to lipid catabolisme1
- Qualifier
- Post-rewateringe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“alfalfa Medicago sativa L.”
e1“Post-rewatering, alfalfa underwent a metabolic shift from energy metabolism to lipid catabolism”
The same questions in other papers
drought · growth · Medicago sativa (alfalfa)Results differ
19 papers in the same direction as this paper · 52 in the opposite direction
- negative
- negative
- negative
68 papers more in this group
See all 72 papers →drought · physiology: hormones · Medicago sativa (alfalfa)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
drought · physiology: photosynthesis · Medicago sativa (alfalfa)Positive and negative results
2 papers in the same direction as this paper · 12 in the opposite direction
- negative
- negative
- no effect
11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.