Abstract
Suberin is a hydrophobic biopolymer that acts as an internal and external diffusion and transpiration barrier in plants. It is involved in two phases of wound healing, i.e. initial closing layer formation and subsequent wound periderm development. Transcriptomic and metabolomic analyses of wounded potato leaf tissue revealed preferential induction of cell wall modifying processes during closing layer formation, accompanied by a highly active defense response. To address the importance of suberin in this process, we generated loss of function mutants by CRISPR-Cas9 editing the suberin transporter gene StABCG1. Both wound-induced StABCG1 transcript levels and suberin formation around wounded leaf tissue were reduced in CRISPR-lines. Moreover, wound-induced tissue damage was characterized by browning of wound-adjacent areas. Transcriptome analyses of these areas revealed up-regulation of genes encoding defense proteins and enzymes of the phenylpropanoid pathway. Levels of hydroxycinnamic acid amides, acting in defense and in cell wall reinforcement, were drastically enhanced in CRISPR compared to control plants. These results suggest that the reduction in suberin formation around wounded tissue leads to a loss of barrier function, resulting in tissue browning due to enhanced exposure to oxygen.
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Claims 5
Claim · model-written summary
CRISPR editing of StABCG1 reduced wound-induced StABCG1 transcript levels and suberin formation around wounded potato leaf tissue.
- Taxon
- potato
Solanum tuberosume0 - Material
- CRISPR-linese1
- Organ / stage
- wounded leaf tissuee1
- Treatment
- CRISPR-Cas9 editinge2
- Gene
- StABCG1e1
- Outcome
- StABCG1 transcript levels and suberin formatione1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“wounded potato leaf tissue”
e1“Both wound-induced StABCG1 transcript levels and suberin formation around wounded leaf tissue were reduced in CRISPR-lines”
e2“we generated loss of function mutants by CRISPR-Cas9 editing the suberin transporter gene StABCG1”
Claim · model-written summary
Wound-induced tissue damage was characterized by browning of wound-adjacent potato leaf areas.
- Taxon
- potatoSolanum tuberosume0
- Organ / stage
- wound-adjacent arease3
- Outcome
- browning of wound-adjacent arease3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“wounded potato leaf tissue”
e3“wound-induced tissue damage was characterized by browning of wound-adjacent areas”
Claim · model-written summary
Defense protein genes and phenylpropanoid pathway enzyme genes were up-regulated in browning areas of wounded potato leaves.
- Taxon
- potatoSolanum tuberosume0
- Organ / stage
- these arease4
- Outcome
- genes encoding defense proteins and enzymes of the phenylpropanoid pathwaye4
- Direction
- up-regulatione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“wounded potato leaf tissue”
e4“Transcriptome analyses of these areas revealed up-regulation of genes encoding defense proteins and enzymes of the phenylpropanoid pathway”
Claim · model-written summary
Hydroxycinnamic acid amide levels were drastically enhanced in CRISPR potato lines compared with control plants.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- CRISPRe5
- Outcome
- hydroxycinnamic acid amidese5
- Direction
- drastically enhancede5
- Comparator
- control plantse5
Not reportedCompound
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“wounded potato leaf tissue”
e5“Levels of hydroxycinnamic acid amides, acting in defense and in cell wall reinforcement, were drastically enhanced in CRISPR compared to control plants”
Claim · model-written summary
Reduced suberin formation around wounded tissue is suggested to cause loss of barrier function and tissue browning in potato.
- Taxon
- potatoSolanum tuberosume0
- Outcome
- tissue browninge6
- Qualifier
- loss of barrier functione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“wounded potato leaf tissue”
e6“the reduction in suberin formation around wounded tissue leads to a loss of barrier function, resulting in tissue browning”
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See all 22 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.