Abstract
Drought can promote soluble sugar accumulation in fruits by increasing the fruit sink strength. Cell wall invertase (CwINV) plays a pivotal role in determining sink strength by regulating sucrose partitioning into the extracellular matrix. Research has demonstrated that drought stress significantly increases the transcript level of citrus CwINV6, but the transcriptional mechanisms governing its regulation under drought conditions remain elusive. In this study, we characterised the MYB transcription factor gene CsMYB1 from the citrus genome. CsMYB1 is localised in the cell nucleus, and CwINV6 is localised in the cell wall. Furthermore, the transcript levels of both CsMYB1 and CwINV6 significantly increased in 'Nanfeng' tangerine fruits (Citrus reticulata) in response to drought or ABA treatment. Transient overexpression of CsMYB1 or CwINV6 promoted the accumulation of glucose and fructose in 'Nanfeng' fruits. Conversely, transient VIGS of CsMYB1 or CwINV6 resulted in the opposite trend. Additionally, stable overexpression of CsMYB1 or CwINV6 significantly increased the soluble sugar content in the fruits of the 'Micro-Tom' tomato lines. Y1H and luciferase assays confirmed that CsMYB1 can bind to the CwINV6 promoter and positively regulate its expression. Taken together, our findings reveal that drought promotes soluble sugar distribution in citrus fruits by increasing sink strength via the CsMYB1-CwINV6 module.
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Claims 11
Claim · model-written summary
CsMYB1 localized to the nucleus.
CitrusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CsMYB1 is localised in the cell nucleus”
t0“Citrus Fruits”
Claim · model-written summary
CwINV6 localized to the cell wall.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CwINV6 is localised in the cell wall”
t0“Citrus Fruits”
Claim · model-written summary
Drought increased CsMYB1 and CwINV6 transcript levels in Nanfeng tangerine fruit.
- Taxon
- Citrus reticulata
.jpg?width=60)
Citrus reticulatae0 - Material
- 'Nanfeng'e0
- Organ / stage
- fruitse0
- Treatment
- droughte0
- Gene
- CsMYB1 and CwINV6e0
- Outcome
- transcript levelse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“the transcript levels of both CsMYB1 and CwINV6 significantly increased in 'Nanfeng' tangerine fruits (Citrus reticulata) in response to drought or ABA treatment”
Claim · model-written summary
ABA increased CsMYB1 and CwINV6 transcript levels in Nanfeng tangerine fruit.
- Taxon
- Citrus reticulataCitrus reticulatae0
- Material
- 'Nanfeng'e0
- Treatment
- ABA treatmente0
- Gene
- CsMYB1 and CwINV6e0
- Outcome
- transcript levelse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transcript levels of both CsMYB1 and CwINV6 significantly increased in 'Nanfeng' tangerine fruits (Citrus reticulata) in response to drought or ABA treatment”
Claim · model-written summary
CsMYB1 overexpression promoted glucose and fructose accumulation in Nanfeng fruit.
- Material
- 'Nanfeng'e0
- Treatment
- Transient overexpression of CsMYB1e0
- Gene
- CsMYB1e0
- Outcome
- accumulation of glucose and fructosee0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient overexpression of CsMYB1 or CwINV6 promoted the accumulation of glucose and fructose in 'Nanfeng' fruits”
t0“Citrus Fruits”
Claim · model-written summary
CwINV6 overexpression promoted glucose and fructose accumulation in Nanfeng fruit.
- Treatment
- overexpression of CsMYB1 or CwINV6e0
- Gene
- CwINV6e0
- Outcome
- accumulation of glucose and fructosee0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient overexpression of CsMYB1 or CwINV6 promoted the accumulation of glucose and fructose in 'Nanfeng' fruits”
t0“Citrus Fruits”
Claim · model-written summary
Silencing CsMYB1 or CwINV6 produced the opposite sugar accumulation trend.
- Treatment
- transient VIGS of CsMYB1 or CwINV6e0
- Gene
- CsMYB1 or CwINV6e0
- Outcome
- opposite trende0
- Direction
- opposite trende0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient VIGS of CsMYB1 or CwINV6 resulted in the opposite trend”
t0“Citrus Fruits”
Claim · model-written summary
CsMYB1 overexpression increased soluble sugar content in Micro-Tom tomato fruit.
- Taxon
- tomato
Solanum lycopersicume0 - Material
- 'Micro-Tom'e0
- Treatment
- stable overexpression of CsMYB1 or CwINV6e0
- Gene
- CsMYB1e0
- Outcome
- soluble sugar contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stable overexpression of CsMYB1 or CwINV6 significantly increased the soluble sugar content in the fruits of the 'Micro-Tom' tomato lines”
Claim · model-written summary
CwINV6 overexpression increased soluble sugar content in Micro-Tom tomato fruit.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- stable overexpression of CsMYB1 or CwINV6e0
- Gene
- CwINV6e0
- Outcome
- soluble sugar contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stable overexpression of CsMYB1 or CwINV6 significantly increased the soluble sugar content in the fruits of the 'Micro-Tom' tomato lines”
Claim · model-written summary
CsMYB1 bound the CwINV6 promoter and positively regulated expression.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CsMYB1 can bind to the CwINV6 promoter and positively regulate its expression”
t0“Citrus Fruits”
Claim · model-written summary
The authors concluded that drought promotes citrus fruit sugar distribution through sink strength via CsMYB1-CwINV6.
- Treatment
- droughte0
- Gene
- CsMYB1-CwINV6e0
- Outcome
- soluble sugar distributione0
- Direction
- promotese0
- Qualifier
- increasing sink strengthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“drought promotes soluble sugar distribution in citrus fruits by increasing sink strength via the CsMYB1-CwINV6 module”
The same questions in other papers
drought · molecular: expression · Citrus reticulata (tangerine)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.