Abstract
Abstract—In two winter garlic (Allium sativum L.) cultivars, the expression of genes encoding key enzymes of the L-galactose pathway of ascorbate (AsA) biosynthesis (VTC2, GPP, GalDH, GalLDH) and genes encoding AsA recycling from oxidized forms (MDHAR1, MDHAR4, MDHAR5) was determined in the dynamics (0, 2, 4, 6, and 24 h) of short-term (24 h) cold (2°C) stress. It was found that in the leaves of both cultivars (Sarmat and Dubkovsky), the level of VTC2 transcripts steadily increased during stress. The cultivars were also similar in the GalLDH and MDHAR1 expression response to stress, but differed in the reaction of the GPP, GalDH, MDHAR4, and MDHAR5. In the leaves of both cultivars, the content of AsA, carotenoids (total) and chlorophylls (a and b) was determined in the dynamics of cold stress. It was shown that the amount of all analyzed metabolites is initially higher in the cv. Sarmat than in the cv. Dubkovsky, with changes in the stress dynamics in a cultivar-specific manner. A positive correlation was shown between the amount of AsA and the level of expression of the GalDH gene for both cultivars.
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Claims 7
Claim · model-written summary
In leaves of both garlic cultivars, VTC2 transcript level steadily increased during cold stress.
- Taxon
- Allium sativum L.
Allium sativumt0 - Treatment
- cold (2°C) stresse0
- Gene
- VTC2e1
- Outcome
- level of VTC2 transcriptse1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Allium sativum L.”
e0“short-term (24 h) cold (2°C) stress”
e1“in the leaves of both cultivars (Sarmat and Dubkovsky), the level of VTC2 transcripts steadily increased during stress”
Claim · model-written summary
The two cultivars were similar in the GalLDH and MDHAR1 expression response to cold stress.
- Taxon
- Allium sativum L.Allium sativumt0
- Treatment
- cold (2°C) stresse0
- Gene
- GalLDH and MDHAR1e1
- Outcome
- expression response to stresse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Allium sativum L.”
e0“short-term (24 h) cold (2°C) stress”
e1“The cultivars were also similar in the GalLDH and MDHAR1 expression response to stress”
Claim · model-written summary
The two cultivars differed in the stress reaction of GPP and GalDH expression.
- Taxon
- Allium sativum L.Allium sativumt0
- Treatment
- cold (2°C) stresse0
- Gene
- GPP, GalDHe1
- Outcome
- differed in the reactione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Allium sativum L.”
e0“short-term (24 h) cold (2°C) stress”
e1“differed in the reaction of the GPP, GalDH, MDHAR4, and MDHAR5”
Claim · model-written summary
The two cultivars differed in the stress reaction of MDHAR4 and MDHAR5 expression.
- Taxon
- Allium sativum L.Allium sativumt0
- Treatment
- cold (2°C) stresse0
- Gene
- MDHAR4, and MDHAR5e1
- Outcome
- differed in the reactione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Allium sativum L.”
e0“short-term (24 h) cold (2°C) stress”
e1“differed in the reaction of the GPP, GalDH, MDHAR4, and MDHAR5”
Claim · model-written summary
The amounts of all analyzed metabolites (AsA, carotenoids, chlorophylls) were initially higher in cv. Sarmat than in cv. Dubkovsky.
- Taxon
- Allium sativum L.Allium sativumt0
- Material
- cv. Sarmate1
- Outcome
- amount of all analyzed metabolitese1
- Direction
- highere1
- Comparator
- cv. Dubkovskye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Allium sativum L.”
e1“the amount of all analyzed metabolites is initially higher in the cv. Sarmat than in the cv. Dubkovsky”
Claim · model-written summary
Metabolite amounts changed during cold stress dynamics in a cultivar-specific manner.
- Taxon
- Allium sativum L.Allium sativumt0
- Outcome
- changes in the stress dynamicse1
- Qualifier
- cultivar-specific mannere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Allium sativum L.”
e1“with changes in the stress dynamics in a cultivar-specific manner”
Claim · model-written summary
AsA amount was positively correlated with GalDH gene expression level in both cultivars.
- Taxon
- Allium sativum L.Allium sativumt0
- Gene
- GalDH genee1
- Outcome
- amount of AsAe1
- Direction
- positive correlatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Allium sativum L.”
e1“A positive correlation was shown between the amount of AsA and the level of expression of the GalDH gene for both cultivars”
The same questions in other papers
cold · molecular: expression · Allium sativum (garlic)Positive and negative results
1 in the opposite direction
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.