Abstract
Abstract Effects of waterlogging (WL) stress and its mitigation by nitrate (NO 3 – ) were investigated in a commercial cucumber hybrid line. WL resulted in a shift toward anaerobic metabolism in roots and development of adventitious roots (ARs). In WL stressed roots exodermal, extracellular H 2 O 2 accumulation and protein nitration were detected. Shoot growth was retarded with signs of oxidative stress. Nitrate content decreased in both root and shoot. In the root WL upregulated several genes implicated in hypoxic response, including transcripts of CsRAP2.3 , CsRBOH s and the CsHem3 phytoglobin genes. Nitrate supplementation restored shoot growth and mitigated oxidative stress. Fermentative metabolism was reduced in the roots. Nitrate levels increased substantially, expression of NRT1 genes was induced, supporting improved NO 3 − uptake and delivery. According to chemical analysis and fluorescent microscopy NO accumulated in the vascular cylinder of roots. NO depletion by cPTIO confirmed a role for NO in stress mitigation. cPTIO treatment decreased AR formation and foliar nitrate levels. It decreased protein nitration and strongly downregulated nitrate transporters’ gene expression in roots. Overall, our work revealed nitrate triggered, NO mediated alleviation of WL stress in cucumber. Resolution of WL induced energy crisis in the roots by nitrate treatment was attributed to parallel effects of anaerobic respiration and AR formation. Shoot growth repression was probably alleviated by improved root functions, including elevated nitrate transport. Expression of NRT1 nitrate transporter genes was induced by nitrate, and this induction was found NO dependent in WL stressed cucumber.
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Claims 19
Claim · model-written summary
Waterlogging upregulated CsRAP2.3 transcripts in cucumber roots.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nitric oxide mediates nitrate induced alleviation of waterlogging stress in cucumber”
e0“investigated in a commercial cucumber hybrid line”
e1“In the root WL upregulated several genes implicated in hypoxic response, including transcripts of CsRAP2.3 , CsRBOH s and the CsHem3 phytoglobin genes”
Claim · model-written summary
Waterlogging upregulated CsRBOHs transcripts in cucumber roots.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nitric oxide mediates nitrate induced alleviation of waterlogging stress in cucumber”
e0“investigated in a commercial cucumber hybrid line”
e1“In the root WL upregulated several genes implicated in hypoxic response, including transcripts of CsRAP2.3 , CsRBOH s and the CsHem3 phytoglobin genes”
Claim · model-written summary
Waterlogging upregulated the CsHem3 phytoglobin gene in cucumber roots.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nitric oxide mediates nitrate induced alleviation of waterlogging stress in cucumber”
e0“investigated in a commercial cucumber hybrid line”
e1“In the root WL upregulated several genes implicated in hypoxic response, including transcripts of CsRAP2.3 , CsRBOH s and the CsHem3 phytoglobin genes”
Claim · model-written summary
Waterlogging shifted cucumber root metabolism toward anaerobic metabolism.
- Taxon
- cucumber
Cucumis sativuse0 - Organ / stage
- rootse1
- Treatment
- WLe1
- Outcome
- anaerobic metabolisme1
- Direction
- shift towarde1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nitric oxide mediates nitrate induced alleviation of waterlogging stress in cucumber”
e0“investigated in a commercial cucumber hybrid line”
e1“WL resulted in a shift toward anaerobic metabolism in roots and development of adventitious roots (ARs)”
Claim · model-written summary
Waterlogging led to development of adventitious roots in cucumber.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- WLe1
- Outcome
- development of adventitious roots (ARs)e1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nitric oxide mediates nitrate induced alleviation of waterlogging stress in cucumber”
e0“investigated in a commercial cucumber hybrid line”
e1“WL resulted in a shift toward anaerobic metabolism in roots and development of adventitious roots (ARs)”
Claim · model-written summary
Waterlogged cucumber roots showed extracellular H2O2 accumulation and protein nitration in the exodermis.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- roots exodermale1
- Treatment
- WLe1
- Outcome
- extracellular H 2 O 2 accumulation and protein nitratione1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nitric oxide mediates nitrate induced alleviation of waterlogging stress in cucumber”
e0“investigated in a commercial cucumber hybrid line”
e1“In WL stressed roots exodermal, extracellular H 2 O 2 accumulation and protein nitration were detected”
Claim · model-written summary
Waterlogging retarded cucumber shoot growth with signs of oxidative stress.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- WLe1
- Outcome
- Shoot growthe2
- Direction
- retardede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nitric oxide mediates nitrate induced alleviation of waterlogging stress in cucumber”
e0“investigated in a commercial cucumber hybrid line”
e1“In WL stressed roots exodermal, extracellular H 2 O 2 accumulation and protein nitration were detected”
e2“Shoot growth was retarded with signs of oxidative stress”
Claim · model-written summary
Waterlogging decreased nitrate content in cucumber root and shoot.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- root and shoote2
- Treatment
- WLe1
- Outcome
- Nitrate contente2
- Direction
- decreasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nitric oxide mediates nitrate induced alleviation of waterlogging stress in cucumber”
e0“investigated in a commercial cucumber hybrid line”
e1“In WL stressed roots exodermal, extracellular H 2 O 2 accumulation and protein nitration were detected”
e2“Nitrate content decreased in both root and shoot”
Claim · model-written summary
Nitrate supplementation restored shoot growth in waterlogged cucumber.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- Nitrate supplementatione1
- Outcome
- shoot growthe1
- Direction
- restorede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nitric oxide mediates nitrate induced alleviation of waterlogging stress in cucumber”
e0“investigated in a commercial cucumber hybrid line”
e1“Nitrate supplementation restored shoot growth and mitigated oxidative stress”
Claim · model-written summary
Nitrate supplementation mitigated oxidative stress in waterlogged cucumber.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- Nitrate supplementatione1
- Outcome
- oxidative stresse1
- Direction
- mitigatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nitric oxide mediates nitrate induced alleviation of waterlogging stress in cucumber”
e0“investigated in a commercial cucumber hybrid line”
e1“Nitrate supplementation restored shoot growth and mitigated oxidative stress”
Claim · model-written summary
Fermentative metabolism was reduced in cucumber roots.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- rootse1
- Outcome
- Fermentative metabolisme1
- Direction
- reducede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nitric oxide mediates nitrate induced alleviation of waterlogging stress in cucumber”
e0“investigated in a commercial cucumber hybrid line”
e1“Fermentative metabolism was reduced in the roots”
Claim · model-written summary
Nitrate levels increased substantially in cucumber under the nitrate treatment.
- Taxon
- cucumberCucumis sativuse0
- Outcome
- Nitrate levelse1
- Direction
- increased substantiallye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nitric oxide mediates nitrate induced alleviation of waterlogging stress in cucumber”
e0“investigated in a commercial cucumber hybrid line”
e1“Nitrate levels increased substantially”
Claim · model-written summary
Expression of NRT1 genes was induced in cucumber under waterlogging with nitrate.
- Taxon
- cucumberCucumis sativuse0
- Gene
- NRT1e1
- Outcome
- expression of NRT1 genese1
- Direction
- inducede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nitric oxide mediates nitrate induced alleviation of waterlogging stress in cucumber”
e0“investigated in a commercial cucumber hybrid line”
e1“expression of NRT1 genes was induced”
Claim · model-written summary
NO accumulated in the vascular cylinder of cucumber roots.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- vascular cylinder of rootse1
- Outcome
- NOe1
- Direction
- accumulatede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nitric oxide mediates nitrate induced alleviation of waterlogging stress in cucumber”
e0“investigated in a commercial cucumber hybrid line”
e1“NO accumulated in the vascular cylinder of roots”
Claim · model-written summary
NO depletion by cPTIO supports a role for NO in stress mitigation in cucumber.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- NO depletion by cPTIOe1
- Compound
- NOe1
- Outcome
- stress mitigatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nitric oxide mediates nitrate induced alleviation of waterlogging stress in cucumber”
e0“investigated in a commercial cucumber hybrid line”
e1“NO depletion by cPTIO confirmed a role for NO in stress mitigation”
Claim · model-written summary
cPTIO treatment decreased adventitious root formation and foliar nitrate levels in cucumber.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- cPTIO treatmente1
- Outcome
- AR formation and foliar nitrate levelse1
- Direction
- decreasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nitric oxide mediates nitrate induced alleviation of waterlogging stress in cucumber”
e0“investigated in a commercial cucumber hybrid line”
e1“cPTIO treatment decreased AR formation and foliar nitrate levels”
Claim · model-written summary
cPTIO decreased protein nitration in cucumber.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- cPTIOe1
- Outcome
- protein nitratione2
- Direction
- decreasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nitric oxide mediates nitrate induced alleviation of waterlogging stress in cucumber”
e0“investigated in a commercial cucumber hybrid line”
e1“NO depletion by cPTIO confirmed a role for NO in stress mitigation”
e2“It decreased protein nitration”
Claim · model-written summary
cPTIO strongly downregulated nitrate transporter genes in cucumber roots.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- cPTIOe1
- Outcome
- nitrate transporterse2
- Direction
- downregulatede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nitric oxide mediates nitrate induced alleviation of waterlogging stress in cucumber”
e0“investigated in a commercial cucumber hybrid line”
e1“NO depletion by cPTIO confirmed a role for NO in stress mitigation”
e2“strongly downregulated nitrate transporters”
Claim · model-written summary
The induction of NRT1 by nitrate in waterlogged cucumber was NO dependent.
- Taxon
- cucumberCucumis sativuse0
- Compound
- NOe1
- Outcome
- inductione1
- Qualifier
- NO dependente1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nitric oxide mediates nitrate induced alleviation of waterlogging stress in cucumber”
e0“investigated in a commercial cucumber hybrid line”
e1“this induction was found NO dependent in WL stressed cucumber”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.