Abstract
Drought ranks among the key abiotic stresses that limit the growth and yield of grapevines (Vitis vinifera L.). Flavonols, a class of antioxidants commonly found in grapevines, play a crucial role in combating drought stress. In this study, we characterized the function and regulatory mechanism of the grapevine VviMYC4 in mediating flavonol biosynthesis in response to drought stress. VviMYC4 encodes a protein of 468 amino acids with conserved bHLH-MYC_N and bHLH domains. Phylogenetic analysis confirmed its homology with the grapevine VviMYC2 and similarity in function. The expression of VviMYC4 in ‘Cabernet Sauvignon’ grapevine seedling leaves increased initially and then decreased during prolonged drought stress. The homologous and heterologous transformation of VviMYC4 in grape suspension cells, Arabidopsis plants, tobacco leaves, and grapevine leaves demonstrated its ability to positively regulate flavonol biosynthesis and accumulation by promoting the expression of flavonol-related genes, thereby enhancing the drought tolerance of transgenic plants. Furthermore, VviMYC4 could bind to specific E-box sites on the promoters of VviF3H and VviFLS to improve their activities. This study highlights VviMYC4 as a pivotal positive regulator of drought tolerance in grapevines and proposes that VviMYC4 enhances the antioxidant and reactive oxygen species (ROS) scavenging abilities of grapevines in challenging environments and improves their stress resilience by mediating flavonol biosynthesis. Our findings offer crucial candidate genes and valuable insights for the molecular breeding of grapevine drought resistance.
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Claims 7
Claim · model-written summary
VviMYC4 encodes a 468 amino acid protein with conserved bHLH domains.
Not reportedTaxon
Verbatim
e0“VviMYC4 encodes a protein of 468 amino acids with conserved bHLH-MYC_N and bHLH domains.”
Claim · model-written summary
Phylogenetic analysis found VviMYC4 homologous to grapevine VviMYC2 with similar function.
VitisNot reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phylogenetic analysis confirmed its homology with the grapevine VviMYC2 and similarity in function.”
Claim · model-written summary
VviMYC4 expression in Cabernet Sauvignon leaves initially increased and then decreased during prolonged drought.
- Material
- Cabernet Sauvignone0
- Organ / stage
- seedling leavese0
- Treatment
- prolonged drought stresse0
- Gene
- VviMYC4e0
- Outcome
- expressione0
- Direction
- increased initially and then decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of VviMYC4 in ‘Cabernet Sauvignon’ grapevine seedling leaves increased initially and then decreased during prolonged drought stress.”
Claim · model-written summary
VviMYC4 transformation increased flavonol biosynthesis and accumulation in the tested plant systems.
- Treatment
- transformation of VviMYC4e0
- Gene
- VviMYC4e0
- Outcome
- flavonol biosynthesis and accumulatione0
- Direction
- positively regulatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The homologous and heterologous transformation of VviMYC4 in grape suspension cells, Arabidopsis plants, tobacco leaves, and grapevine leaves demonstrated its ability to positively regulate flavonol biosynthesis and accumulation by promoting the expression of flavonol-related genes, thereby enhancing the drought tolerance of transgenic plants.”
Claim · model-written summary
VviMYC4 transformation promoted expression of flavonol-related genes.
- Treatment
- transformation of VviMYC4e0
- Gene
- VviMYC4e0
- Outcome
- expression of flavonol-related genese0
- Direction
- promotinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The homologous and heterologous transformation of VviMYC4 in grape suspension cells, Arabidopsis plants, tobacco leaves, and grapevine leaves demonstrated its ability to positively regulate flavonol biosynthesis and accumulation by promoting the expression of flavonol-related genes, thereby enhancing the drought tolerance of transgenic plants.”
Claim · model-written summary
VviMYC4 transformation enhanced drought tolerance of transgenic plants.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The homologous and heterologous transformation of VviMYC4 in grape suspension cells, Arabidopsis plants, tobacco leaves, and grapevine leaves demonstrated its ability to positively regulate flavonol biosynthesis and accumulation by promoting the expression of flavonol-related genes, thereby enhancing the drought tolerance of transgenic plants.”
Claim · model-written summary
VviMYC4 bound E-box promoter sites of VviF3H and VviFLS and improved promoter activity.
- Gene
- VviMYC4e0
- Outcome
- activitiese0
- Direction
- improvee0
- Qualifier
- specific E-box sites on the promoters of VviF3H and VviFLSe0
Not reportedTaxon
Verbatim
e0“VviMYC4 could bind to specific E-box sites on the promoters of VviF3H and VviFLS to improve their activities.”
The same questions in other papers
transgenic · molecular: expression · VitisPositive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.