Abstract
Summary Responsive to desiccation 21 (RD21) is a papain‐like cysteine protease (PLCP). PLCPs are involved in plant growth and development, as well as stress and plant immune responses. Currently, few studies have reported on the involvement of PLCPs in defence against viral infections. Here, we show that RD21 of strawberry ( Fragaria vesca ) interacts with the strawberry vein banding virus (SVBV) P6 protein. SVBV infection induces FvRD21 expression. Subcellular localization revealed that FvRD21 and P6 colocalize in the perinuclear and cytoplasmic regions. Further analysis showed that FvRD21 overexpression induces the autophagy pathway that degrades the P6 protein, destroys the function of P6 as a viral suppressor of RNA silencing (VSR), inhibits the pathogenicity of P6, and confers resistance to SVBV in strawberry plants. Moreover, mutant FvRD21 H300A lost its ability to interact with SVBV P6 and did not affect P6 accumulation and VSR function. Additionally, abscisic acid (ABA) pretreatment of strawberry plants promoted FvRD21 upregulation and inhibited SVBV infection, and FvRD21 overexpression activated the ABA pathway. In summary, FvRD21 inhibits SVBV infection by degrading SVBV P6 through the autophagy pathway. Hence, this study provides a novel report on the defence strategy of strawberry plants against SVBV infections.
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Claims 13
Claim · model-written summary
Strawberry RD21 interacts with the strawberry vein banding virus P6 protein.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassae0 - Interactor
- strawberry vein banding virus (SVBV)
Strawberry vein banding viruse0pathogen - Gene
- RD21e0
- Outcome
- interacts with the strawberry vein banding virus (SVBV) P6 proteine0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“RD21 of strawberry ( Fragaria vesca ) interacts with the strawberry vein banding virus (SVBV) P6 protein”
Claim · model-written summary
SVBV infection induced FvRD21 expression.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- SVBV infectione1
- Interactor
- SVBVStrawberry vein banding viruse1pathogen
- Outcome
- FvRD21 expressione1
- Direction
- inducese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RD21 of strawberry ( Fragaria vesca )”
e1“SVBV infection induces FvRD21 expression”
Claim · model-written summary
FvRD21 and P6 colocalized in the perinuclear and cytoplasmic regions.
- Taxon
- strawberryFragaria ×ananassae0
- Gene
- FvRD21e1
- Outcome
- colocalizee1
- Qualifier
- perinuclear and cytoplasmic regionse1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RD21 of strawberry ( Fragaria vesca )”
e1“FvRD21 and P6 colocalize in the perinuclear and cytoplasmic regions”
Claim · model-written summary
FvRD21 overexpression induced the autophagy pathway that degrades the viral P6 protein.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- FvRD21 overexpressione0
- Outcome
- autophagy pathway that degrades the P6 proteine0
- Direction
- inducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FvRD21 overexpression induces the autophagy pathway that degrades the P6 protein, destroys the function of P6 as a viral suppressor of RNA silencing (VSR), inhibits the pathogenicity of P6, and confers resistance to SVBV in strawberry plants”
Claim · model-written summary
FvRD21 overexpression destroyed the function of P6 as a viral suppressor of RNA silencing.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- FvRD21 overexpressione0
- Outcome
- function of P6 as a viral suppressor of RNA silencing (VSR)e0
- Direction
- destroyse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FvRD21 overexpression induces the autophagy pathway that degrades the P6 protein, destroys the function of P6 as a viral suppressor of RNA silencing (VSR), inhibits the pathogenicity of P6, and confers resistance to SVBV in strawberry plants”
Claim · model-written summary
FvRD21 overexpression inhibited the pathogenicity of P6.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- FvRD21 overexpressione0
- Outcome
- pathogenicity of P6e0
- Direction
- inhibitse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FvRD21 overexpression induces the autophagy pathway that degrades the P6 protein, destroys the function of P6 as a viral suppressor of RNA silencing (VSR), inhibits the pathogenicity of P6, and confers resistance to SVBV in strawberry plants”
Claim · model-written summary
FvRD21 overexpression conferred resistance to SVBV in strawberry plants.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- FvRD21 overexpressione0
- Interactor
- SVBVStrawberry vein banding viruse0pathogen
- Outcome
- resistance to SVBVe0
- Direction
- conferse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FvRD21 overexpression induces the autophagy pathway that degrades the P6 protein, destroys the function of P6 as a viral suppressor of RNA silencing (VSR), inhibits the pathogenicity of P6, and confers resistance to SVBV in strawberry plants”
Claim · model-written summary
Mutant FvRD21 H300A lost its ability to interact with SVBV P6.
- Taxon
- strawberryFragaria ×ananassae0
- Material
- FvRD21 H300Ae1
- Outcome
- lost its ability to interact with SVBV P6e1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RD21 of strawberry ( Fragaria vesca )”
e1“mutant FvRD21 H300A lost its ability to interact with SVBV P6”
Claim · model-written summary
Mutant FvRD21 H300A did not affect P6 accumulation or VSR function.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- mutant FvRD21 H300Ae1
- Outcome
- P6 accumulation and VSR functione1
- Direction
- did not affecte1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RD21 of strawberry ( Fragaria vesca )”
e1“mutant FvRD21 H300A lost its ability to interact with SVBV P6 and did not affect P6 accumulation and VSR function”
Claim · model-written summary
Abscisic acid (ABA) pretreatment of strawberry plants promoted FvRD21 upregulation.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- abscisic acid (ABA) pretreatmente0
- Outcome
- FvRD21 upregulatione0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“abscisic acid (ABA) pretreatment of strawberry plants promoted FvRD21 upregulation and inhibited SVBV infection”
Claim · model-written summary
Abscisic acid (ABA) pretreatment of strawberry plants inhibited SVBV infection.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- abscisic acid (ABA) pretreatmente0
- Interactor
- SVBVStrawberry vein banding viruse0pathogen
- Outcome
- SVBV infectione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“abscisic acid (ABA) pretreatment of strawberry plants promoted FvRD21 upregulation and inhibited SVBV infection”
Claim · model-written summary
FvRD21 overexpression activated the ABA pathway.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- FvRD21 overexpressione1
- Outcome
- ABA pathwaye1
- Direction
- activatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RD21 of strawberry ( Fragaria vesca )”
e1“FvRD21 overexpression activated the ABA pathway”
Claim · model-written summary
The authors conclude that FvRD21 inhibits SVBV infection by degrading SVBV P6 through the autophagy pathway.
- Taxon
- strawberryFragaria ×ananassae0
- Interactor
- SVBVStrawberry vein banding viruse1pathogen
- Gene
- FvRD21e1
- Outcome
- SVBV infectione1
- Qualifier
- degrading SVBV P6 through the autophagy pathwaye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RD21 of strawberry ( Fragaria vesca )”
e1“FvRD21 inhibits SVBV infection by degrading SVBV P6 through the autophagy pathway”
The same questions in other papers
(+)-abscisic acid · molecular: expression · Fragaria x ananassa (strawberry)Positive and negative results
7 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- no effect
- positive
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.