Paper · 2025 · Seed Research

Enhancing Seedling Growth and Budding Potential in Ber (Zizyphus mauritiana) Using Gibberellic Acid and Bacterial Inoculants

GULAB PANDOVE; NAVJOT GUPTA

7
claims
24
verbatim quotes
0
citations
3
research areas
✓
Open access

Abstract

Indian jujube (Zizyphus mauritiana Lamk.), commonly known as ber, is a commercially significant fruit in arid and semi-arid regions of India due to its high returns, low input costs, and drought tolerance. However, delayed budding in ber rootstock seedlings often results in unbuddable plants, leading to resource wastage. This study aimed to enhance vegetative growth in ber rootstock under nursery conditions using gibberellic acid (GA3) and liquid bacterial inoculants (LBIs) of Sphingobacterium sp. and Azotobacter sp. A field experiment was conducted at PAU, Regional Research Station, Bathinda, using a randomized complete block design with five treatments. Seedlings treated with the combined LBIs showed significant improvements in shoot number (27.45%), shoot length (21.04%), collar diameter (29.63%), root length (17.65%), and biomass accumulation compared to control.Enhanced growth is attributed to increased nutrient availability and phytohormone production by inoculated bacteria. Although microbial counts in rhizospheric soil increased numerically in treated plants, the changes were statistically non-significant. The findings suggest that LBIs can effectively improve rootstock growth, making seedlings suitablefor timely budding and reducing wastage. Future studies should assess budding success and conduct biosafety evaluations of the microbial strains, alongside soil biofertilizer applications to improve long-term soil health and productivity

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Claims 7

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Combined liquid bacterial inoculants significantly improve shoot number of ber seedlings compared with control (27.45%).

Taxon
Zizyphus mauritianaZiziphus mauritianae0
Treatment
combined LBIse1
Interactor
Sphingobacterium sp.Sphingobacteriume2symbiont
Second interactor
Azotobacter sp.Azotobactere2symbiont
Outcome
shoot numbere1
Direction
improvementse1
Value
27.45%e1
Comparator
controle1
Setting
PAU, Regional Research Station, Bathindae3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ziziphus mauritiana
Ziziphus mauritiana · TaxonEthel Aardvark · CC BY 3.0 · Wikimedia Commons ↗
Sphingobacterium
Sphingobacterium · Interactor · picture from its Wikimedia Commons categoryAjay Kumar Chaurasiya · CC BY-SA 4.0 · Wikimedia Commons ↗
Azotobacter
Azotobacter · Second interactor · picture of the genus (one of its species)DAN H. JONES · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Indian jujube (Zizyphus mauritiana Lamk.), commonly known as ber”

✓ located verbatim in the source · abstract, characters 0–64 · show in text ↑
e1

“Seedlings treated with the combined LBIs showed significant improvements in shoot number (27.45%), shoot length (21.04%), collar diameter (29.63%), root length (17.65%), and biomass accumulation compared to control”

✓ located verbatim in the source · abstract, characters 664–878 · show in text ↑
e2

“liquid bacterial inoculants (LBIs) of Sphingobacterium sp. and Azotobacter sp.”

✓ located verbatim in the source · abstract, characters 444–522 · show in text ↑
e3

“A field experiment was conducted at PAU, Regional Research Station, Bathinda”

✓ located verbatim in the source · abstract, characters 523–599 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Combined liquid bacterial inoculants significantly improve shoot length of ber seedlings compared with control (21.04%).

Taxon
Zizyphus mauritianaZiziphus mauritianae0
Treatment
combined LBIse1
Interactor
Sphingobacterium sp.Sphingobacteriume2symbiont
Second interactor
Azotobacter sp.Azotobactere2symbiont
Outcome
shoot lengthe1
Direction
improvementse1
Value
21.04%e1
Comparator
controle1
Setting
PAU, Regional Research Station, Bathindae3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ziziphus mauritiana
Ziziphus mauritiana · TaxonEthel Aardvark · CC BY 3.0 · Wikimedia Commons ↗
Sphingobacterium
Sphingobacterium · Interactor · picture from its Wikimedia Commons categoryAjay Kumar Chaurasiya · CC BY-SA 4.0 · Wikimedia Commons ↗
Azotobacter
Azotobacter · Second interactor · picture of the genus (one of its species)DAN H. JONES · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Indian jujube (Zizyphus mauritiana Lamk.), commonly known as ber”

✓ located verbatim in the source · abstract, characters 0–64 · show in text ↑
e1

“Seedlings treated with the combined LBIs showed significant improvements in shoot number (27.45%), shoot length (21.04%), collar diameter (29.63%), root length (17.65%), and biomass accumulation compared to control”

✓ located verbatim in the source · abstract, characters 664–878 · show in text ↑
e2

“liquid bacterial inoculants (LBIs) of Sphingobacterium sp. and Azotobacter sp.”

✓ located verbatim in the source · abstract, characters 444–522 · show in text ↑
e3

“A field experiment was conducted at PAU, Regional Research Station, Bathinda”

✓ located verbatim in the source · abstract, characters 523–599 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Combined liquid bacterial inoculants significantly improve collar diameter of ber seedlings compared with control (29.63%).

Taxon
Zizyphus mauritianaZiziphus mauritianae0
Treatment
combined LBIse1
Interactor
Sphingobacterium sp.Sphingobacteriume2symbiont
Second interactor
Azotobacter sp.Azotobactere2symbiont
Outcome
collar diametere1
Direction
improvementse1
Value
29.63%e1
Comparator
controle1
Setting
PAU, Regional Research Station, Bathindae3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ziziphus mauritiana
Ziziphus mauritiana · TaxonEthel Aardvark · CC BY 3.0 · Wikimedia Commons ↗
Sphingobacterium
Sphingobacterium · Interactor · picture from its Wikimedia Commons categoryAjay Kumar Chaurasiya · CC BY-SA 4.0 · Wikimedia Commons ↗
Azotobacter
Azotobacter · Second interactor · picture of the genus (one of its species)DAN H. JONES · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Indian jujube (Zizyphus mauritiana Lamk.), commonly known as ber”

✓ located verbatim in the source · abstract, characters 0–64 · show in text ↑
e1

“Seedlings treated with the combined LBIs showed significant improvements in shoot number (27.45%), shoot length (21.04%), collar diameter (29.63%), root length (17.65%), and biomass accumulation compared to control”

✓ located verbatim in the source · abstract, characters 664–878 · show in text ↑
e2

“liquid bacterial inoculants (LBIs) of Sphingobacterium sp. and Azotobacter sp.”

✓ located verbatim in the source · abstract, characters 444–522 · show in text ↑
e3

“A field experiment was conducted at PAU, Regional Research Station, Bathinda”

✓ located verbatim in the source · abstract, characters 523–599 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Combined liquid bacterial inoculants significantly improve root length of ber seedlings compared with control (17.65%).

Taxon
Zizyphus mauritianaZiziphus mauritianae0
Treatment
combined LBIse1
Interactor
Sphingobacterium sp.Sphingobacteriume2symbiont
Second interactor
Azotobacter sp.Azotobactere2symbiont
Outcome
root lengthe1
Direction
improvementse1
Value
17.65%e1
Comparator
controle1
Setting
PAU, Regional Research Station, Bathindae3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ziziphus mauritiana
Ziziphus mauritiana · TaxonEthel Aardvark · CC BY 3.0 · Wikimedia Commons ↗
Sphingobacterium
Sphingobacterium · Interactor · picture from its Wikimedia Commons categoryAjay Kumar Chaurasiya · CC BY-SA 4.0 · Wikimedia Commons ↗
Azotobacter
Azotobacter · Second interactor · picture of the genus (one of its species)DAN H. JONES · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Indian jujube (Zizyphus mauritiana Lamk.), commonly known as ber”

✓ located verbatim in the source · abstract, characters 0–64 · show in text ↑
e1

“Seedlings treated with the combined LBIs showed significant improvements in shoot number (27.45%), shoot length (21.04%), collar diameter (29.63%), root length (17.65%), and biomass accumulation compared to control”

✓ located verbatim in the source · abstract, characters 664–878 · show in text ↑
e2

“liquid bacterial inoculants (LBIs) of Sphingobacterium sp. and Azotobacter sp.”

✓ located verbatim in the source · abstract, characters 444–522 · show in text ↑
e3

“A field experiment was conducted at PAU, Regional Research Station, Bathinda”

✓ located verbatim in the source · abstract, characters 523–599 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Combined liquid bacterial inoculants significantly improve biomass accumulation of ber seedlings compared with control.

Taxon
Zizyphus mauritianaZiziphus mauritianae0
Treatment
combined LBIse1
Interactor
Sphingobacterium sp.Sphingobacteriume2symbiont
Second interactor
Azotobacter sp.Azotobactere2symbiont
Outcome
biomass accumulatione1
Direction
improvementse1
Comparator
controle1
Setting
PAU, Regional Research Station, Bathindae3

Not reportedValue

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ziziphus mauritiana
Ziziphus mauritiana · TaxonEthel Aardvark · CC BY 3.0 · Wikimedia Commons ↗
Sphingobacterium
Sphingobacterium · Interactor · picture from its Wikimedia Commons categoryAjay Kumar Chaurasiya · CC BY-SA 4.0 · Wikimedia Commons ↗
Azotobacter
Azotobacter · Second interactor · picture of the genus (one of its species)DAN H. JONES · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Indian jujube (Zizyphus mauritiana Lamk.), commonly known as ber”

✓ located verbatim in the source · abstract, characters 0–64 · show in text ↑
e1

“Seedlings treated with the combined LBIs showed significant improvements in shoot number (27.45%), shoot length (21.04%), collar diameter (29.63%), root length (17.65%), and biomass accumulation compared to control”

✓ located verbatim in the source · abstract, characters 664–878 · show in text ↑
e2

“liquid bacterial inoculants (LBIs) of Sphingobacterium sp. and Azotobacter sp.”

✓ located verbatim in the source · abstract, characters 444–522 · show in text ↑
e3

“A field experiment was conducted at PAU, Regional Research Station, Bathinda”

✓ located verbatim in the source · abstract, characters 523–599 · show in text ↑
Mechanismauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

Enhanced ber seedling growth is attributed to increased nutrient availability and phytohormone production by the inoculated bacteria.

Taxon
Zizyphus mauritianaZiziphus mauritianae0
Outcome
Enhanced growthe4
Qualifier
increased nutrient availability and phytohormone productione4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ziziphus mauritiana
Ziziphus mauritiana · TaxonEthel Aardvark · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Indian jujube (Zizyphus mauritiana Lamk.), commonly known as ber”

✓ located verbatim in the source · abstract, characters 0–64 · show in text ↑
e4

“Enhanced growth is attributed to increased nutrient availability and phytohormone production by inoculated bacteria”

✓ located verbatim in the source · abstract, characters 879–994 · show in text ↑
Observationobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Microbial counts in rhizospheric soil of treated ber plants increased numerically but the change was not statistically significant.

Taxon
Zizyphus mauritianaZiziphus mauritianae0
Treatment
treated plantse5
Outcome
microbial counts in rhizospheric soile5
Direction
increased numericallye5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ziziphus mauritiana
Ziziphus mauritiana · TaxonEthel Aardvark · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Indian jujube (Zizyphus mauritiana Lamk.), commonly known as ber”

✓ located verbatim in the source · abstract, characters 0–64 · show in text ↑
e5

“Although microbial counts in rhizospheric soil increased numerically in treated plants, the changes were statistically non-significant”

✓ located verbatim in the source · abstract, characters 996–1130 · show in text ↑