Paper · 2025 · Plants

Intercropping Lettuce with Alfalfa Under Variable Nitrate Supply: Effects on Growth Performance and Nutrient Dynamics in a Vertical Hydroponic System

Luis D-Andrade; Nivia Escalante-Garcia; Ernesto Olvera-Gonzalez; et al.

5
claims
10
verbatim quotes
4
citations
2
research areas
✓
Open access · cc-by

Abstract

Vertical farming systems offer an efficient solution for sustainable food production in urban areas. However, managing nitrate (NO3−) levels remains a significant challenge for improving crop yield, quality, and safety. This study evaluated the effects of nitrate availability on growth performance, nutrient uptake, and water use efficiency in a vertical hydroponic system that intercropped lettuce (Lactuca sativa) with alfalfa (Medicago sativa). The experiment was conducted in a controlled vertical hydroponic system using Nutrient Film Technique (NFT) channels, with nitrogen levels set at 0, 33, 66, 100, and 133% of the standard concentration. The results indicated that the intercropping treatment with 66% nitrate (IC-N66%) improved water use efficiency by 38% and slightly increased leaf area compared to the other intercropping treatments. However, the control group, which consisted of a monoculture with full nitrate supply, achieved the highest overall biomass. Ion concentrations, including nitrate, calcium, magnesium, and micronutrients, were moderately affected by the intercropping strategy and nitrate levels. These findings suggest that moderate nitrate input, combined with nitrogen-fixing legumes, can enhance resource efficiency in hydroponic systems without significantly compromising yield. These findings offer a promising framework for incorporating legumes into hydroponic systems, minimizing the need for synthetic inputs while maintaining yield. These results support the use of agroecological intensification strategies in highly efficient soilless systems.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 5

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Intercropping at 66% nitrate improved water use efficiency by 38% versus other intercropping treatments.

Taxon
LettuceLactuca sativat0
Treatment
intercropping treatment with 66% nitrate (IC-N66%)e0
Outcome
water use efficiencye0
Direction
improvede0
Value
38%e0
Comparator
other intercropping treatmentse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“the intercropping treatment with 66% nitrate (IC-N66%) improved water use efficiency by 38% and slightly increased leaf area compared to the other intercropping treatments.”

✓ located verbatim in the source · abstract, characters 678–850 · show in text ↑
t0

“Lettuce with Alfalfa”

✓ located verbatim in the source · title, characters 14–34 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Intercropping at 66% nitrate slightly increased leaf area.

Taxon
LettuceLactuca sativat0
Treatment
intercropping treatment with 66% nitrate (IC-N66%)e0
Outcome
leaf areae0
Direction
increasede0
Qualifier
slightlye0
Comparator
other intercropping treatmentse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“the intercropping treatment with 66% nitrate (IC-N66%) improved water use efficiency by 38% and slightly increased leaf area compared to the other intercropping treatments.”

✓ located verbatim in the source · abstract, characters 678–850 · show in text ↑
t0

“Lettuce with Alfalfa”

✓ located verbatim in the source · title, characters 14–34 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Full nitrate monoculture had highest overall biomass.

Taxon
LettuceLactuca sativat0
Treatment
monoculture with full nitrate supplye0
Outcome
overall biomasse0
Direction
higheste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“the control group, which consisted of a monoculture with full nitrate supply, achieved the highest overall biomass.”

✓ located verbatim in the source · abstract, characters 860–975 · show in text ↑
t0

“Lettuce with Alfalfa”

✓ located verbatim in the source · title, characters 14–34 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Intercropping and nitrate levels moderately affected ion concentrations.

Taxon
LettuceLactuca sativat0
Treatment
intercropping strategy and nitrate levelse0
Outcome
Ion concentrationse0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“Ion concentrations, including nitrate, calcium, magnesium, and micronutrients, were moderately affected by the intercropping strategy and nitrate levels.”

✓ located verbatim in the source · abstract, characters 976–1129 · show in text ↑
t0

“Lettuce with Alfalfa”

✓ located verbatim in the source · title, characters 14–34 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors suggest moderate nitrate input with legumes can improve resource efficiency without significantly compromising yield.

Taxon
LettuceLactuca sativat0
Outcome
resource efficiency in hydroponic systemse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“moderate nitrate input, combined with nitrogen-fixing legumes, can enhance resource efficiency in hydroponic systems without significantly compromising yield.”

✓ located verbatim in the source · abstract, characters 1158–1316 · show in text ↑
t0

“Lettuce with Alfalfa”

✓ located verbatim in the source · title, characters 14–34 · show in text ↑

nitrogen · growth · Lactuca sativa (garden lettuce)Results differ

85 papers: 60 positive, 25 negative or no effect

59 papers in the same direction as this paper · 22 in the opposite direction · 3 reporting no difference from an alternative

  1. no effect
  2. negative
  3. no effect

81 papers more in this group

See all 85 papers →

intercropping · growth · Lactuca sativa (garden lettuce)Positive and negative results

5 papers: 2 positive, 3 negative or no effect

1 paper in the same direction as this paper · 3 in the opposite direction

  1. negative
  2. negative
  3. no effect

1 paper more in this group

See all 5 papers →

rotation · growth · Lactuca sativa (garden lettuce)Positive and negative results

6 papers: 3 positive, 3 negative or no effect

2 papers in the same direction as this paper · 2 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. no effect
  3. no effect

2 papers more in this group

See all 6 papers →

nitrogen · physiology: water · Lactuca sativa (garden lettuce)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.