Abstract
Fusarium crown rot (FCR) is one of the most serious soil-borne diseases in common wheat and has caused major wheat yield losses worldwide. Here, we identified an 18.6 kDa heat shock protein gene ( TaHSP18.6 ) through combining a transcriptome analysis and a genome-wide association study. We verified the positive role of TaHSP18.6 in regulating wheat FCR resistance using ethyl methanesulfonate (EMS) mutants and genetic transformation. Next, we screened a lysine deacetylase sirtuin-like (TaSRT1) to determine its potential interaction with TaHSP18.6. We demonstrated that TaSRT1 deacetylated TaHSP18.6 and thereby inhibited TaHSP18.6 protein accumulation. Haplotype analysis revealed that the K171M substitution of TaHSP18.6 generated a susceptible haplotype TaHSP18.6 M171 in wheat, and mass spectrometry results implied that K171 is a key lysine acetylation site. We confirmed the differential acetylation level between TaHSP18.6 K171 and TaHSP18.6 M171 by TaSRT1. Analysis of overexpression lines and EMS mutants showed that TaSRT1 negatively regulated wheat FCR resistance. Meanwhile, we identified that TaHSP18.6 interacted with an auxin-responsive protein IAA1 (TaIAA1) that negatively regulated FCR resistance. TaHSP18.6 overexpression and TaSRT1 mutation significantly increased the auxin content. Exogenous application of auxin substantially enhanced wheat FCR resistance. Taken together, we proposed a TaSRT1–TaHSP18.6 model regulating FCR resistance possibly through mediating TaIAA1 to change the endogenous auxin content in wheat plants.
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Claims 15
Claim · model-written summary
Transcriptome and association analyses identified TaHSP18.6.
- Taxon
- common wheat
Triticum aestivumt0 - Gene
- TaHSP18.6e0
- Outcome
- identified an 18.6 kDa heat shock protein genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified an 18.6 kDa heat shock protein gene ( TaHSP18.6 ) through combining a transcriptome analysis and a genome-wide association study”
t0“common wheat”
Claim · model-written summary
TaHSP18.6 positively regulated wheat Fusarium crown rot resistance.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaHSP18.6e0
- Outcome
- FCR resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the positive role of TaHSP18.6 in regulating wheat FCR resistance”
t0“common wheat”
Claim · model-written summary
TaSRT1 interacted with TaHSP18.6.
- Taxon
- common wheatTriticum aestivumt0
- Gene
- TaSRT1e1
- Outcome
- interaction with TaHSP18.6e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its potential interaction with TaHSP18.6”
e1“a lysine deacetylase sirtuin-like (TaSRT1)”
t0“common wheat”
Claim · model-written summary
TaSRT1 deacetylated TaHSP18.6.
- Taxon
- common wheatTriticum aestivumt0
- Gene
- TaSRT1e0
- Outcome
- deacetylated TaHSP18.6e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaSRT1 deacetylated TaHSP18.6”
t0“common wheat”
Claim · model-written summary
TaSRT1 inhibited TaHSP18.6 protein accumulation.
- Taxon
- common wheatTriticum aestivumt0
- Gene
- TaSRT1e0
- Outcome
- TaHSP18.6 protein accumulatione0
- Direction
- inhibitede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaSRT1 deacetylated TaHSP18.6 and thereby inhibited TaHSP18.6 protein accumulation”
t0“common wheat”
Claim · model-written summary
The K171M substitution generated the susceptible TaHSP18.6 M171 haplotype.
- Taxon
- wheatTriticum aestivume0
- Material
- TaHSP18.6 M171e0
- Gene
- TaHSP18.6e0
- Outcome
- susceptible haplotypee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the K171M substitution of TaHSP18.6 generated a susceptible haplotype TaHSP18.6 M171 in wheat”
Claim · model-written summary
Mass spectrometry implied K171 is a key lysine acetylation site.
- Taxon
- common wheatTriticum aestivumt0
- Gene
- K171e0
- Outcome
- key lysine acetylation sitee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mass spectrometry results implied that K171 is a key lysine acetylation site”
t0“common wheat”
Claim · model-written summary
TaSRT1 acetylated TaHSP18.6 K171 and M171 differentially.
- Taxon
- common wheatTriticum aestivumt0
- Gene
- TaSRT1e0
- Outcome
- differential acetylation level between TaHSP18.6 K171 and TaHSP18.6 M171e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“differential acetylation level between TaHSP18.6 K171 and TaHSP18.6 M171 by TaSRT1”
t0“common wheat”
Claim · model-written summary
TaSRT1 negatively regulated wheat Fusarium crown rot resistance.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaSRT1e0
- Outcome
- FCR resistancee0
- Direction
- negativelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaSRT1 negatively regulated wheat FCR resistance”
Claim · model-written summary
TaHSP18.6 interacted with TaIAA1.
- Taxon
- common wheatTriticum aestivumt0
- Gene
- TaHSP18.6e0
- Outcome
- interacted with an auxin-responsive protein IAA1 (TaIAA1)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaHSP18.6 interacted with an auxin-responsive protein IAA1 (TaIAA1)”
t0“common wheat”
Claim · model-written summary
TaIAA1 negatively regulated Fusarium crown rot resistance.
- Taxon
- common wheatTriticum aestivumt0
- Gene
- TaIAA1e0
- Outcome
- FCR resistancee0
- Direction
- negativelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaIAA1) that negatively regulated FCR resistance”
t0“common wheat”
Claim · model-written summary
TaHSP18.6 overexpression significantly increased auxin content.
- Taxon
- common wheatTriticum aestivumt0
- Treatment
- TaHSP18.6 overexpressione0
- Outcome
- auxin contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaHSP18.6 overexpression and TaSRT1 mutation significantly increased the auxin content”
t0“common wheat”
Claim · model-written summary
TaSRT1 mutation significantly increased auxin content.
- Taxon
- common wheatTriticum aestivumt0
- Treatment
- TaSRT1 mutatione0
- Outcome
- auxin contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaHSP18.6 overexpression and TaSRT1 mutation significantly increased the auxin content”
t0“common wheat”
Claim · model-written summary
Applied auxin substantially enhanced wheat Fusarium crown rot resistance.
- Taxon
- wheatTriticum aestivume0
- Treatment
- Exogenous application of auxine0
- Outcome
- FCR resistancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous application of auxin substantially enhanced wheat FCR resistance”
Claim · model-written summary
The authors propose TaSRT1–TaHSP18.6 regulates resistance through TaIAA1-mediated changes in endogenous auxin.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaSRT1–TaHSP18.6e0
- Outcome
- FCR resistancee0
- Qualifier
- possibly through mediating TaIAA1 to change the endogenous auxin contente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a TaSRT1–TaHSP18.6 model regulating FCR resistance possibly through mediating TaIAA1 to change the endogenous auxin content in wheat plants”
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