Abstract
Sclerotinia sclerotiorum, a devastating necrotrophic fungus causing stem rot in oilseed rape, results in significant global yield losses. Deciphering plant-S. sclerotiorum interactions is crucial for disease control. Here, we report that the transcription factor BnaWRKY75 positively regulates resistance to this pathogen. BnaWRKY75 was the most highly induced WRKY gene upon S. sclerotiorum infection. Overexpressing BnaA10.WRKY75 in both Arabidopsis and oilseed rape enhanced resistance to S. sclerotiorum, whereas BnaWRKY75 knockout plants exhibited reduced resistance. RNA sequencing and DNA affinity purification sequencing showed that the isochorismate synthase gene BnaICS1, a key gene required for salicylic acid (SA) biosynthesis, is a potential target of BnaA10.WRKY75. Yeast one-hybrid, dual-luciferase, and electrophoresis mobility shift assays indicated that BnaA10.WRKY75 directly binds to the BnaCnn.ICS1 promoter to activate its transcription, thereby promoting SA biosynthesis and activating SA-dependent defenses. Genetic analysis in Arabidopsis confirmed ICS1 functions downstream of BnaA10.WRKY75. SA treatment significantly enhanced resistance to S. sclerotiorum in oilseed rape, further highlighting the importance of SA in defense against this pathogen. Finally, we observed differences in transcriptional regulation among BnaICS1 homologous genes in allotetraploid oilseed rape. Our findings suggest that the BnaWRKY75-BnaICS1 module regulates SA-dependent resistance to S. sclerotiorum, expanding our understanding of plant immune responses to necrotrophic pathogens.
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Claims 11
Claim · model-written summary
BnaWRKY75 positively regulates resistance to Sclerotinia sclerotiorum.
- Taxon
- oilseed rape
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napuse1 - Interactor
- Sclerotinia sclerotiorum
Sclerotinia sclerotiorume1pathogen - Gene
- BnaWRKY75e0
- Outcome
- resistancee0
- Direction
- positivelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“the transcription factor BnaWRKY75 positively regulates resistance to this pathogen”
e1“Sclerotinia sclerotiorum, a devastating necrotrophic fungus causing stem rot in oilseed rape”
Claim · model-written summary
Sclerotinia infection induced BnaWRKY75 more strongly than other WRKY genes.
- Interactor
- S. sclerotiorumSclerotinia sclerotiorume0pathogen
- Gene
- BnaWRKY75e0
- Outcome
- WRKY genee0
- Direction
- most highly inducede0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BnaWRKY75 was the most highly induced WRKY gene upon S. sclerotiorum infection”
Claim · model-written summary
BnaA10.WRKY75 overexpression enhanced Arabidopsis resistance to Sclerotinia.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- Overexpressing BnaA10.WRKY75e0
- Interactor
- S. sclerotiorumSclerotinia sclerotiorume0pathogen
- Gene
- BnaA10.WRKY75e0
- Outcome
- resistancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpressing BnaA10.WRKY75 in both Arabidopsis and oilseed rape enhanced resistance to S. sclerotiorum”
Claim · model-written summary
BnaA10.WRKY75 overexpression enhanced oilseed rape resistance to Sclerotinia.
- Taxon
- oilseed rapeBrassica napuse0
- Treatment
- Overexpressing BnaA10.WRKY75e0
- Interactor
- S. sclerotiorumSclerotinia sclerotiorume0pathogen
- Gene
- BnaA10.WRKY75e0
- Outcome
- resistancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpressing BnaA10.WRKY75 in both Arabidopsis and oilseed rape enhanced resistance to S. sclerotiorum”
Claim · model-written summary
BnaWRKY75 knockout reduced resistance.
Not reportedTaxonComparator
Verbatim
e0“BnaWRKY75 knockout plants exhibited reduced resistance”
Claim · model-written summary
Sequencing implicated BnaICS1 as a possible BnaA10.WRKY75 target.
Not reportedTaxonDirection
Verbatim
e0“RNA sequencing and DNA affinity purification sequencing showed that the isochorismate synthase gene BnaICS1, a key gene required for salicylic acid (SA) biosynthesis, is a potential target of BnaA10.WRKY75”
Claim · model-written summary
BnaA10.WRKY75 binds the BnaCnn.ICS1 promoter and activates its transcription.
Not reportedTaxon
Verbatim
e0“BnaA10.WRKY75 directly binds to the BnaCnn.ICS1 promoter to activate its transcription”
Claim · model-written summary
BnaA10.WRKY75 promoted SA biosynthesis and SA-dependent defense.
Not reportedTaxon
Verbatim
e0“thereby promoting SA biosynthesis and activating SA-dependent defenses”
Claim · model-written summary
Arabidopsis genetic analysis placed ICS1 downstream of BnaA10.WRKY75.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- ICS1e0
- Outcome
- functions downstream of BnaA10.WRKY75e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Genetic analysis in Arabidopsis confirmed ICS1 functions downstream of BnaA10.WRKY75”
Claim · model-written summary
Salicylic acid treatment significantly enhanced oilseed rape resistance to Sclerotinia.
- Taxon
- oilseed rapeBrassica napuse0
- Treatment
- SA treatmente0
- Interactor
- S. sclerotiorumSclerotinia sclerotiorume0pathogen
- Outcome
- resistancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SA treatment significantly enhanced resistance to S. sclerotiorum in oilseed rape”
Claim · model-written summary
Oilseed rape BnaICS1 homologues differed in transcriptional regulation.
- Taxon
- oilseed rapeBrassica napuse0
- Gene
- BnaICS1 homologous genese0
- Outcome
- transcriptional regulatione0
- Direction
- differencese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“differences in transcriptional regulation among BnaICS1 homologous genes in allotetraploid oilseed rape”