Abstract
Micropropagation is an efficient method for rapid asexual propagation, production of disease-free plants, and access to propagating materials throughout the year for olive plants. In this study, with the aim of investigation different methods of olive micropropagation, explants of Kroneiki cultivar from olive trees were cultured on different basal culture media, MS (Murashige and Skoog), OM (Olive Medium), DKW (Driver and Kuniyki), and WPM (Woody Plant Medium) without plant growth regulators. Then, the explants were cultured on basal culture media enriched with auxins NAA (Naphthalene acetic acid, IAA (Indole acetic acid) and IBA (Indole butyric acid)) and cytokinins BA (Benzyl adenine), KIN (Kinetin), 2ip (Isopentenyl adenine), TDZ (Thidiazuron), and ZEA (Zeatin) at concentrations of 0.05 to 7.38 μM/L. The superiority of DKW basal culture medium and ZEA growth regulator for shoot induction and longitudinal growth of induced shoot was proven. With increasing concentration of growth regulators, especially BA, the callus reaction was dominant over other reactions. Different concentrations of growth regulators NAA, IAA and IBA (0.05 to 8 μM/L) were used for rooting which was often unsuccessful. Finally, the rooting stage of the regenerated shoots was achieved by soaking the shoots (the basal part of the shoots) in NAA solution (37.5 μM/L for 5 minutes) and transferring them directly to the soil.
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Claims 5
Claim · model-written summary
DKW medium was superior for olive shoot induction and longitudinal growth.
- Taxon
- Olive
Olea europaeat0 - Treatment
- DKW basal culture mediume0
- Outcome
- shoot induction and longitudinal growth of induced shoote0
- Direction
- superioritye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The superiority of DKW basal culture medium and ZEA growth regulator for shoot induction and longitudinal growth of induced shoot was proven.”
t0“Olive”
Claim · model-written summary
ZEA was superior for olive shoot induction and longitudinal growth.
- Taxon
- OliveOlea europaeat0
- Treatment
- ZEA growth regulatore0
- Outcome
- shoot induction and longitudinal growth of induced shoote0
- Direction
- superioritye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The superiority of DKW basal culture medium and ZEA growth regulator for shoot induction and longitudinal growth of induced shoot was proven.”
t0“Olive”
Claim · model-written summary
Increasing growth regulator concentration, especially BA, favored callus formation over other responses.
- Taxon
- OliveOlea europaeat0
- Treatment
- increasing concentration of growth regulators, especially BAe0
- Outcome
- callus reactione0
- Direction
- dominante0
- Comparator
- other reactionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“With increasing concentration of growth regulators, especially BA, the callus reaction was dominant over other reactions.”
t0“Olive”
Claim · model-written summary
Rooting with tested concentrations of NAA, IAA and IBA was often unsuccessful.
- Taxon
- OliveOlea europaeat0
- Treatment
- NAA, IAA and IBA (0.05 to 8 μM/L)e0
- Outcome
- rootinge0
- Direction
- often unsuccessfule0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Different concentrations of growth regulators NAA, IAA and IBA (0.05 to 8 μM/L) were used for rooting which was often unsuccessful.”
t0“Olive”
Claim · model-written summary
Soaking regenerated olive shoots in NAA before soil transfer achieved rooting.
- Taxon
- OliveOlea europaeat0
- Treatment
- soaking the shoots (the basal part of the shoots) in NAA solution (37.5 μM/L for 5 minutes) and transferring them directly to the soile0
- Outcome
- rooting stagee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the rooting stage of the regenerated shoots was achieved by soaking the shoots (the basal part of the shoots) in NAA solution (37.5 μM/L for 5 minutes) and transferring them directly to the soil.”
t0“Olive”
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