Abstract
Considerable effort is being directed at the recovery of superior olive cultivars through conventional breeding. However, the development of biotechnological tools could speed up the development of new cultivars with superior traits, e.g., enhanced disease resistance. In this investigation, attempts have been made to develop a protocol that would allow genetic transformation of juvenile material from the cultivar 'Picual'. Initially, embryogenic cultures were established from mature seeds using radicle segments as explants. The effects of two different mineral formulations OMc vs. OMe on growth and maintenance of embryogenic cultures were evaluated. Results showed that although higher growth rate could be obtained with the OMc mineral formulation, a more organized growth occurred with the formulation with lower macroelements content (OMe). Moreover, culturing the embryogenic cultures for 3 weeks in liquid OMe medium followed by subculture of the fine fraction derived from the resulting suspension on a medium without growth regulators but supplemented with activated charcoal, allowed the development of white-opaque embryos, some of which could be converted into plants. For transformation of embryogenic cultures via particle bombardment, 3 different plasmids containing the GUS marker gene under the control of different promoters were used: pGUSINT (including the 35S promoter), pJGUSS (35S promoter with an enhancer) and pCGUΔ1 (sunflower ubiquitin promoter). The highest number of blue spots was obtained with the ubiquitin promoter. Hence, it has been selected for transformation of this cultivar. Experiments are now in progress to establish the most adequate conditions for selection of fully transformed somatic embryos.
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Claims 4
Claim · model-written summary
OMc mineral formulation produced a higher growth rate of olive embryogenic cultures.
- Taxon
- OLIVE
Olea europaeat0 - Material
- Picualt1
- Treatment
- OMc mineral formulatione0
- Outcome
- growth ratee0
- Direction
- highere0
- Comparator
- OMet1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher growth rate could be obtained with the OMc mineral formulation”
t0“OLIVE”
t1“cultivar 'Picual'. Initially, embryogenic cultures were established from mature seeds using radicle segments as explants. The effects of two different mineral formulations OMc vs. OMe”
Claim · model-written summary
OMe produced more organized growth of olive embryogenic cultures.
- Taxon
- OLIVEOlea europaeat0
- Treatment
- OMee0
- Outcome
- organized growthe0
- Direction
- moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a more organized growth occurred with the formulation with lower macroelements content (OMe)”
t0“OLIVE”
Claim · model-written summary
Liquid OMe culture followed by subculture produced white-opaque embryos, some convertible into plants.
- Taxon
- OLIVEOlea europaeat0
- Treatment
- culturing the embryogenic cultures for 3 weeks in liquid OMe medium followed by subculture of the fine fraction derived from the resulting suspension on a medium without growth regulators but supplemented with activated charcoale0
- Outcome
- development of white-opaque embryose0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“culturing the embryogenic cultures for 3 weeks in liquid OMe medium followed by subculture of the fine fraction derived from the resulting suspension on a medium without growth regulators but supplemented with activated charcoal, allowed the development of white-opaque embryos, some of which could be converted into plants”
t0“OLIVE”
Claim · model-written summary
The ubiquitin promoter produced the most blue spots in olive embryogenic cultures.
- Taxon
- OLIVEOlea europaeat0
- Gene
- ubiquitin promotere0
- Outcome
- number of blue spotse0
- Direction
- higheste0
- Comparator
- ubiquitin promotere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest number of blue spots was obtained with the ubiquitin promoter”
t0“OLIVE”
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